A modulated template-matching approach to improve spike sorting of bursting neurons.

In extracellular neural electrophysiology, individual spikes have to be assigned to their cell of origin in a procedure called "spike sorting". Spike sorting is an unsupervised problem, since no ground-truth information is generally available. Here, we focus on improving spike sorting performance, particularly during periods of high synchronous activity or so-called "bursting". Bursting entails systematic changes in spike shapes and amplitudes and remains a challenge for current spike sorting schemes. We use realistic simulated bursting recordings of high-density micro-electrode arrays (HD-MEAs) and we present a fully automated algorithm based on template matching with a focus on recovering missed spikes during bursts. To compare and benchmark spike-sorting performance after applying our method, we used ground-truth information of simulated recordings. We show that our approach can be effective in improving spike sorting performance during bursting. Further validation with experimental recordings is necessary.