Andina Setyawati1,2, Mae Sri Hartati Wahyuningsih3, Dwi Aris Agung Nugrahaningsih3, Christantie Effendy4, Firas Fneish5, Gerhard Fortwengel6. 1. Lecturer of Department of Surgical and Medical Nursing, Faculty of Nursing, Universitas Hasanuddin, Jl. Perintis Kemerdekaan km 10, Kampus Tamalanrea, Makassar 90245, Indonesia. 2. Student of Department of Medicine and Health Science Doctorate Program, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Jl. Senolowo, Sekip Utara, Depok, Sleman, Yogyakarta 55281, Indonesia. 3. Lecturer of Department of Pharmacology and Therapy, Centre for Herbal Medicine, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Jl. Senolowo, Sekip Utara, Depok, Sleman, Yogyakarta 55281, Indonesia. 4. Lecturer of Department of Surgical and Medical Nursing, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Jl. Senolowo, Sekip Utara, Depok, Sleman, Yogyakarta 55281, Indonesia. 5. Lecturer of Department of Biostatistics, Gottfried Wilhelm Leibniz Universität, Postfach 6009, 30060 Hannover, Germany. 6. Lecturer of Department of Clinical Research and Epidemiology, Hochschule Hannover University of Applied Sciences & Arts, Expo Plaza 12, 30539 Hannover, Germany.
Abstract
INTRODUCTION: Piper crocatum Ruiz & Pav (P. crocatum) has been reported to accelerate the diabetic wound healing process empirically. Some studies showed the benefits of P. crocatum in treating various diseases but its mechanisms in diabetic wound healing have never been reported. In the present study we investigated the diabetic wound healing activity of the active fraction of P. crocatum on wounded hyperglycemia fibroblasts (wHFs). METHODS: Bioassay-guided fractionation was performed to get the most active fraction. The selected active fraction was applied to wHFs within 72 h incubation. Mimicking a diabetic condition was done using basal glucose media containing an additional 17 mMol/L D-glucose. A wound was simulated via the scratch assay. The collagen deposition was measured using Picro-Sirius Red and wound closure was measured using scratch wound assay. Underlying mechanisms through p53, αSMA, SOD1 and E-cadherin were measured using western blotting. RESULTS: We reported that FIV is the most active fraction of P. crocatum. We confirmed that FIV \(7.81 µg/ml, 15.62 µg/ml, 31.25 µg/ml, 62.5 µg/ml, and 125 µg/ml) induced the collagen deposition and wound closure of wHFs. Furthermore, FIV treatment (7.81 µg/ml, 15.62 µg/ml, 31.25 µg/ml) down-regulated the protein expression level of p53 and up-regulated the protein expression levels of αSMA, E-cadherin, and SOD1. DISCUSSION/ CONCLUSIONS: Our findings suggest that ameliorating collagen deposition and wound closure through protein regulation of p53, αSMA, E-cadherin, and SOD1 are some of the mechanisms by which FIV of P. crocatum is involved in diabetic wound healing therapy.
INTRODUCTION: Piper crocatum Ruiz & Pav (P. crocatum) has been reported to accelerate the diabetic wound healing process empirically. Some studies showed the benefits of P. crocatum in treating various diseases but its mechanisms in diabetic wound healing have never been reported. In the present study we investigated the diabetic wound healing activity of the active fraction of P. crocatum on wounded hyperglycemia fibroblasts (wHFs). METHODS: Bioassay-guided fractionation was performed to get the most active fraction. The selected active fraction was applied to wHFs within 72 h incubation. Mimicking a diabetic condition was done using basal glucose media containing an additional 17 mMol/L D-glucose. A wound was simulated via the scratch assay. The collagen deposition was measured using Picro-Sirius Red and wound closure was measured using scratch wound assay. Underlying mechanisms through p53, αSMA, SOD1 and E-cadherin were measured using western blotting. RESULTS: We reported that FIV is the most active fraction of P. crocatum. We confirmed that FIV \(7.81 µg/ml, 15.62 µg/ml, 31.25 µg/ml, 62.5 µg/ml, and 125 µg/ml) induced the collagen deposition and wound closure of wHFs. Furthermore, FIV treatment (7.81 µg/ml, 15.62 µg/ml, 31.25 µg/ml) down-regulated the protein expression level of p53 and up-regulated the protein expression levels of αSMA, E-cadherin, and SOD1. DISCUSSION/ CONCLUSIONS: Our findings suggest that ameliorating collagen deposition and wound closure through protein regulation of p53, αSMA, E-cadherin, and SOD1 are some of the mechanisms by which FIV of P. crocatum is involved in diabetic wound healing therapy.
Authors: Xin Huang; Bin Meng; Javeed Iqbal; B Belinda Ding; Anamarija M Perry; Wenfeng Cao; Lynette M Smith; Chengfeng Bi; Chunsun Jiang; Timothy C Greiner; Dennis D Weisenburger; Lisa Rimsza; Andreas Rosenwald; German Ott; Jan Delabie; Elias Campo; Rita M Braziel; Randy D Gascoyne; James R Cook; Raymond R Tubbs; Elaine S Jaffe; James O Armitage; Julie M Vose; Louis M Staudt; Timothy W McKeithan; Wing C Chan; B Hilda Ye; Kai Fu Journal: J Clin Oncol Date: 2013-11-12 Impact factor: 44.544
Authors: Luz E Gasca-Lozano; Silvia Lucano-Landeros; Héctor Ruiz-Mercado; Adriana Salazar-Montes; Ana Sandoval-Rodríguez; Jesus Garcia-Bañuelos; Arturo Santos-Garcia; Judith R Davila-Rodriguez; José Navarro-Partida; Hiram Bojórquez-Sepúlveda; Juan Castañeda-Gomez; José Domínguez-Rosales; Myriam A Ruiz-Arcos; María Guadalupe Sánchez-Parada; Juan Armendariz-Borunda Journal: J Diabetes Res Date: 2017-12-31 Impact factor: 4.011