Fatma Kübra Tombulturk1,2, Tugba Soydas1,3, Elif Yaprak Sarac4, Matem Tuncdemir1, Ender Coskunpinar5, Erdal Polat6, Serhat Sirekbasan7, Gonul Kanigur-Sultuybek8. 1. Department of Medical Biology, Cerrahpasa Medical Faculty, Istanbul University-Cerrahpasa, Istanbul, Turkey. 2. Medical Laboratory Techniques, Vocational School of Health Services, Istinye University, Istanbul, Turkey. 3. Department of Medical Biology and Genetics, Istanbul Aydin University, Medical Faculty, Sefakoy-Kucukcekmece, 34295, Istanbul, Turkey. 4. Department of Histology and Embryology, Istanbul Medical Faculty, Istanbul University, Istanbul, Turkey. 5. Department of Medical Biology, Medical Faculty, Saglik Bilimleri University, Istanbul, Turkey. 6. Department of Medical Microbiology, Cerrahpasa Medical Faculty, Istanbul University-Cerrahpasa, Istanbul, Turkey. 7. Department of Biotherapy Research and Development Laboratory, Istanbul University, Istanbul, Turkey. 8. Department of Medical Biology and Genetics, Istanbul Aydin University, Medical Faculty, Sefakoy-Kucukcekmece, 34295, Istanbul, Turkey. kanigur952@gmail.com.
Abstract
AIMS: Lucilia sericata larvae have been successfully used on healing of wounds in the diabetics. However, the involvement of the extraction/secretion (ES) products of larvae in the treatment of diabetic wounds is still unknown. Activator protein-1 (AP-1) transcription, composed of c-jun and c-Fos proteins, has been shown to be the principal regulator of multiple MMP transcriptions under a variety of conditions, also in diabetic wounds. Specifically, MMP-2 and MMP-9's transcriptions are known to be modulated by AP-1. c-jun has been demonstrated to be a repressor of p53 in immortalized fibroblasts. The aim of the present study is to investigate the effects of L. sericata ES on the expression of AP-1 (c-jun), p53, MMP-2, and MMP-9 in wound biopsies dissected from streptozotocin induced diabetic rats. METHODS: The expression levels of MMP-2, MMP-9, c-jun and p53 in dermal tissues were determined at days 0, 3, 7 and 14 after wounding, using immunohistochemical analysis and quantitative real-time PCR. RESULTS: The treatment with ES significantly decreased through inflammation-based induction of MMP-2 and MMP-9 expression levels in the wounds of diabetic groups, compared to control groups at the third day of wound healing. At the 14th day, there were dramatic decreases in expression of c-jun, MMP-9, and p53 in ES-treated groups, compared to the diabetic group (P < 0.001, P < 0.05 and P < 0.01, respectively). CONCLUSION: ES products of L. sericata may enhance the process of wound healing in phases of inflammation, proliferation, and re-epithelization, essentially via regulating c-jun expression and modulating MMP-2 and MMP-9 expressions.
AIMS: Lucilia sericata larvae have been successfully used on healing of wounds in the diabetics. However, the involvement of the extraction/secretion (ES) products of larvae in the treatment of diabetic wounds is still unknown. Activator protein-1 (AP-1) transcription, composed of c-jun and c-Fos proteins, has been shown to be the principal regulator of multiple MMP transcriptions under a variety of conditions, also in diabetic wounds. Specifically, MMP-2 and MMP-9's transcriptions are known to be modulated by AP-1. c-jun has been demonstrated to be a repressor of p53 in immortalized fibroblasts. The aim of the present study is to investigate the effects of L. sericataES on the expression of AP-1 (c-jun), p53, MMP-2, and MMP-9 in wound biopsies dissected from streptozotocin induced diabeticrats. METHODS: The expression levels of MMP-2, MMP-9, c-jun and p53 in dermal tissues were determined at days 0, 3, 7 and 14 after wounding, using immunohistochemical analysis and quantitative real-time PCR. RESULTS: The treatment with ES significantly decreased through inflammation-based induction of MMP-2 and MMP-9 expression levels in the wounds of diabetic groups, compared to control groups at the third day of wound healing. At the 14th day, there were dramatic decreases in expression of c-jun, MMP-9, and p53 in ES-treated groups, compared to the diabetic group (P < 0.001, P < 0.05 and P < 0.01, respectively). CONCLUSION:ES products of L. sericata may enhance the process of wound healing in phases of inflammation, proliferation, and re-epithelization, essentially via regulating c-jun expression and modulating MMP-2 and MMP-9 expressions.
Authors: Cristian Loretelli; Moufida Ben Nasr; Giorgio Giatsidis; Roberto Bassi; Luca Lancerotto; Francesca D'Addio; Alessandro Valderrama-Vasquez; Saja Sandra Scherer; Luca Salvatore; Marta Madaghiele; Ahmed Abdelsalam; Elio Ippolito; Emma Assi; Vera Usuelli; Basset El Essawy; Alessandro Sannino; Giorgio Pietramaggiori; Gian Vincenzo Zuccotti; Dennis Paul Orgill; Paolo Fiorina Journal: Acta Diabetol Date: 2020-03-02 Impact factor: 4.280
Authors: Miho Takahashi; Yoshie Umehara; Hainan Yue; Juan Valentin Trujillo-Paez; Ge Peng; Hai Le Thanh Nguyen; Risa Ikutama; Ko Okumura; Hideoki Ogawa; Shigaku Ikeda; François Niyonsaba Journal: Front Immunol Date: 2021-09-14 Impact factor: 7.561