| Literature DB >> 34845703 |
Konstantina Katsarou1,2, Nikoleta Kryovrysanaki1,2, Kriton Kalantidis3,4.
Abstract
Viroids are considered the most minimalistic group of pathogens. Despite their presumed inability to encode for proteins, viroids induce several diseases in plants of primary economic importance. The production of viroid derived siRNAs (vd-siRNAs) of 21-24 nt, accompanies viroid infections in plants and results from the activation of the RNA silencing mechanism and specifically the function of Dicer endonucleases. A comprehensive set of experiments for the study and thorough analysis of viroid-infected plants has been developed. Here we present a detailed experimental plan including optimized protocols for plant infection by agroinfiltration, RNA extraction, and northern blot hybridization for the detection of both viroid genomic RNA and vd-siRNAs.Entities:
Keywords: Agroinfiltration; Northern blot; RNA extraction; Viroid; vd-siRNAs
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Year: 2022 PMID： 34845703 DOI： 10.1007/978-1-0716-1464-8_24
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745