Literature DB >> 3478676

Cloning and expression of T4 DNA polymerase.

T C Lin1, J Rush, E K Spicer, W H Konigsberg.   

Abstract

The structural gene coding for bacteriophage T4 DNA polymerase (gene 43) has been cloned into inducible plasmid vectors, which provide a source for obtaining large amounts of this enzyme after induction. The T4 DNA polymerase produced in this fashion was purified by an innovative three-step procedure and was fully active.

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Year:  1987        PMID： 3478676      PMCID： PMC299216          DOI： 10.1073/pnas.84.20.7000

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  24 in total

1.  Molecular cloning of the DNA ligase gene from bacteriophage T4. I. Characterisation of the recombinants.

Authors:  G G Wilson; N E Murray
Journal:  J Mol Biol       Date:  1979-08-15       Impact factor: 5.469

2.  DNA replication by bacteriophage T4 proteins. The T4 43, 32, 44--62, And 45 proteins are required for strand displacement synthesis at nicks in duplex DNA.

Authors:  N G Nossal; B M Peterlin
Journal:  J Biol Chem       Date:  1979-07-10       Impact factor: 5.157

3.  Control of bacteriophage T4 DNA polymerase synthesis.

Authors:  M Russel
Journal:  J Mol Biol       Date:  1973-09-05       Impact factor: 5.469

4.  Enzymatic synthesis of deoxyribonucleic acid. 36. A proofreading function for the 3' leads to 5' exonuclease activity in deoxyribonucleic acid polymerases.

Authors:  D Brutlag; A Kornberg
Journal:  J Biol Chem       Date:  1972-01-10       Impact factor: 5.157

5.  Genetic mapping of the amino-terminal domain of bacteriophage T4 DNA polymerase.

Authors:  M B Hughes; A M Yee; M Dawson; J Karam
Journal:  Genetics       Date:  1987-03       Impact factor: 4.562

6.  A procedure for the rapid, large-scall purification of Escherichia coli DNA-dependent RNA polymerase involving Polymin P precipitation and DNA-cellulose chromatography.

Authors:  R R Burgess; J J Jendrisak
Journal:  Biochemistry       Date:  1975-10-21       Impact factor: 3.162

7.  Fidelity of DNA replication catalysed in vitro on a natural DNA template by the T4 bacteriophage multi-enzyme complex.

Authors:  U Hibner; B M Alberts
Journal:  Nature       Date:  1980-05-29       Impact factor: 49.962

8.  Efficient in vitro replication of double-stranded DNA templates by a purified T4 bacteriophage replication system.

Authors:  N K Sinha; C F Morris; B M Alberts
Journal:  J Biol Chem       Date:  1980-05-10       Impact factor: 5.157

9.  Regulation of the synthesis of the T4 DNA polymerase (gene 43).

Authors:  R C Miller; E T Young; R H Epstein; H M Krisch; T Mattson; A Bolle
Journal:  Virology       Date:  1981-04-15       Impact factor: 3.616

10.  The DNA enzymology of protein machines.

Authors:  B M Alberts
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1984
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  13 in total

1.  Purification and characterization of recombinant tissue kallikrein from Escherichia coli and yeast.

Authors:  J Wang; J Chao; L Chao
Journal:  Biochem J       Date:  1991-05-15       Impact factor: 3.857

Review 2.  Enzymatic labeling of nucleic acids.

Authors:  J Temsamani; S Agrawal
Journal:  Mol Biotechnol       Date:  1996-06       Impact factor: 2.695

3.  Identification of a transient excision intermediate at the crossroads between DNA polymerase extension and proofreading pathways.

Authors:  R P Baker; L J Reha-Krantz
Journal:  Proc Natl Acad Sci U S A       Date:  1998-03-31       Impact factor: 11.205

Review 4.  A new look at old mutants of T4 DNA polymerase.

Authors:  N G Nossal
Journal:  Genetics       Date:  1998-04       Impact factor: 4.562

Review 5.  Type II restriction endonucleases--a historical perspective and more.

Authors:  Alfred Pingoud; Geoffrey G Wilson; Wolfgang Wende
Journal:  Nucleic Acids Res       Date:  2014-05-30       Impact factor: 16.971

Review 6.  Bacteriophage T4 genome.

Authors:  Eric S Miller; Elizabeth Kutter; Gisela Mosig; Fumio Arisaka; Takashi Kunisawa; Wolfgang Rüger
Journal:  Microbiol Mol Biol Rev       Date:  2003-03       Impact factor: 11.056

7.  Re-engineering the polymerase domain of Klenow fragment and evaluation of overproduction and purification strategies.

Authors:  V Derbyshire; M Astatke; C M Joyce
Journal:  Nucleic Acids Res       Date:  1993-11-25       Impact factor: 16.971

8.  DNA polymerization in the absence of exonucleolytic proofreading: in vivo and in vitro studies.

Authors:  L J Reha-Krantz; S Stocki; R L Nonay; E Dimayuga; L D Goodrich; W H Konigsberg; E K Spicer
Journal:  Proc Natl Acad Sci U S A       Date:  1991-03-15       Impact factor: 11.205

9.  Construction and characterization of a bacteriophage T4 DNA polymerase deficient in 3'-->5' exonuclease activity.

Authors:  M W Frey; N G Nossal; T L Capson; S J Benkovic
Journal:  Proc Natl Acad Sci U S A       Date:  1993-04-01       Impact factor: 11.205

10.  Bacteriophage T4 DNA polymerase mutations that confer sensitivity to the PPi analog phosphonoacetic acid.

Authors:  L J Reha-Krantz; R L Nonay; S Stocki
Journal:  J Virol       Date:  1993-01       Impact factor: 5.103
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