| Literature DB >> 34618227 |
A Debernardi1, W Jarassier2, C Soret1,2, C Mougin1,2, S Alizon3, I G Bravo3,4, D Guenat1,2, J L Prétet1,2, Q Lepiller5,6,7.
Abstract
Simple and standardized approaches for genome analysis of human papillomavirus (HPV) by next-generation sequencing are needed. The aim of the study was to develop a protocol for direct deep sequencing of high-risk (hr) HPV strains, based on the widely used commercial Hybrid Capture 2 (QIAGEN) test, without any additional probe design. This protocol was applied to 15 HPV-positive and two HPV-negative cervical samples or cell lines and validated at the genotype level by comparing the sequencing results to those obtained using a commercial genotyping kit. The performance of our protocol, presented in this proof-of-principle study, supports its use for accurate characterization of genetic variants of hrHPV.Entities:
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Year: 2021 PMID： 34618227 DOI： 10.1007/s00705-021-05259-9
Source DB: PubMed Journal: Arch Virol ISSN： 0304-8608 Impact factor: 2.574