Literature DB >> 34541242

Biotinylated Micro-RNA Pull Down Assay for Identifying miRNA Targets.

Pornima Phatak1,2, James M Donahue1,2.   

Abstract

microRNA (miRNA) directly associates with its target transcripts (mRNA). This protocol describes a method for detection of direct interaction between miRNA and mRNA. The result of interaction helps screening the specific target mRNAs for a miRNA.
Copyright © 2017 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  Biotin labelling; Biotin-pulldown; Transfection; miRNA; miRNA-mRNA interaction

Year:  2017        PMID： 34541242      PMCID： PMC8410240          DOI： 10.21769/BioProtoc.2253

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  3 in total

1.  Isolation of microRNA targets using biotinylated synthetic microRNAs.

Authors:  Ulf Andersson Orom; Anders H Lund
Journal:  Methods       Date:  2007-10       Impact factor: 3.608

2.  MicroRNAs down-regulate homologous recombination in the G1 phase of cycling cells to maintain genomic stability.

Authors:  Young Eun Choi; Yunfeng Pan; Eunmi Park; Panagiotis Konstantinopoulos; Subhajyoti De; Alan D'Andrea; Dipanjan Chowdhury
Journal:  Elife       Date:  2014-04-30       Impact factor: 8.140

3.  Overexpression of miR-214-3p in esophageal squamous cancer cells enhances sensitivity to cisplatin by targeting survivin directly and indirectly through CUG-BP1.

Authors:  P Phatak; K A Byrnes; D Mansour; L Liu; S Cao; R Li; J N Rao; D J Turner; J-Y Wang; J M Donahue
Journal:  Oncogene       Date:  2015-08-03       Impact factor: 9.867
  3 in total
  8 in total

1.  miR-340-5p Alleviates Oxidative Stress Injury by Targeting MyD88 in Sepsis-Induced Cardiomyopathy.

Authors:  Cong Zhang; Lijin Zeng; Guoyi Cai; Yuanting Zhu; Yan Xiong; Hong Zhan; Zhen Yang
Journal:  Oxid Med Cell Longev       Date:  2022-05-04       Impact factor: 7.310

2.  A Circ-0007022/miR-338-3p/Neuropilin-1 Axis Reduces the Radiosensitivity of Esophageal Squamous Cell Carcinoma by Activating Epithelial-To-Mesenchymal Transition and PI3K/AKT Pathway.

Authors:  Junpeng Zhang; Yanyan Yu; Xiaoyang Yin; Lei Feng; Zhe Li; Xiaomeng Liu; Xinshuang Yu; Baosheng Li
Journal:  Front Genet       Date:  2022-04-29       Impact factor: 4.772

3.  Linc01133 contributes to gastric cancer growth by enhancing YES1-dependent YAP1 nuclear translocation via sponging miR-145-5p.

Authors:  Yujing Sun; Yuan Tian; Junyi He; Yaru Tian; Guohao Zhang; Ruinan Zhao; Wen-Jie Zhu; Peng Gao
Journal:  Cell Death Dis       Date:  2022-01-11       Impact factor: 8.469

4.  Circular RNA hsa_circ_0075542 acts as a sponge for microRNA-1197 to suppress malignant characteristics and promote apoptosis in prostate cancer cells.

Authors:  Yuefu Han; Xingqiao Wen; Xiaojuan Li; Dong Chen; Lian Peng; Bin Lai; Hongcai Huang
Journal:  Bioengineered       Date:  2021-12       Impact factor: 3.269

5.  Retracted Article: Long non-coding RNA XIST promotes proliferation, autophagy and inhibits apoptosis by regulating microRNA-30c/ATG5 axis in gastric cancer.

Authors:  Mingjian Liu; Hongbo Zhao; Min Sheng; Hui Li
Journal:  RSC Adv       Date:  2018-11-07       Impact factor: 4.036

6.  MiR-4739 inhibits the malignant behavior of esophageal squamous cell carcinoma cells via the homeobox C10/vascular endothelial growth factor A/phosphatidylinositol 3-kinase/AKT pathway.

Authors:  Kaiqin Wu; Zhenchuan Liu; Chenglai Dong; Shaorui Gu; Lei Li; Wenli Wang; Yongxin Zhou
Journal:  Bioengineered       Date:  2022-06       Impact factor: 6.832

7.  MicroRNA let-7 Suppresses Influenza A Virus Infection by Targeting RPS16 and Enhancing Type I Interferon Response.

Authors:  Wenjiao Wu; Chao Wang; Changliang Xia; Shuwen Liu; Qinghua Mei
Journal:  Front Cell Infect Microbiol       Date:  2022-07-07       Impact factor: 6.073

8.  miR-539-5p regulates Srebf1 transcription in the skeletal muscle of diabetic mice by targeting DNA methyltransferase 3b.

Authors:  Devesh Kesharwani; Amit Kumar; Ashima Rizvi; Malabika Datta
Journal:  Mol Ther Nucleic Acids       Date:  2022-08-13       Impact factor: 10.183
  8 in total

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