Literature DB >> 17889804

Isolation of microRNA targets using biotinylated synthetic microRNAs.

Ulf Andersson Orom1, Anders H Lund.   

Abstract

MicroRNAs are small regulatory RNAs found in multicellular organisms where they post-transcriptionally regulate gene expression. In animals, microRNAs bind mRNAs via incomplete base pairings making the identification of microRNA targets inherently difficult. Here, we present a detailed method for experimental identification of microRNA targets based on affinity purification of tagged microRNAs associated with their targets.

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Year:  2007        PMID： 17889804     DOI： 10.1016/j.ymeth.2007.04.007

Source DB:  PubMed          Journal:  Methods        ISSN： 1046-2023            Impact factor:   3.608


  82 in total

1.  The potential role of microRNAs in regulating gonadal sex differentiation in the chicken embryo.

Authors:  Andrew D Cutting; Stephanie C Bannister; Tim J Doran; Andrew H Sinclair; Mark V L Tizard; Craig A Smith
Journal:  Chromosome Res       Date:  2012-01       Impact factor: 5.239

2.  Identification of microRNA target genes in vivo.

Authors:  Wei Zheng; Hua-Wei Zou; Yong-Gang Tan; Wei-Song Cai
Journal:  Mol Biotechnol       Date:  2011-03       Impact factor: 2.695

3.  Affinity purification of microRNA-133a with the cardiac transcription factor, Hand2.

Authors:  Ngan K Vo; Ryan P Dalton; Ning Liu; Eric N Olson; Richard H Goodman
Journal:  Proc Natl Acad Sci U S A       Date:  2010-10-25       Impact factor: 11.205

Review 4.  Desperately seeking microRNA targets.

Authors:  Marshall Thomas; Judy Lieberman; Ashish Lal
Journal:  Nat Struct Mol Biol       Date:  2010-10       Impact factor: 15.369

5.  Post-transcriptional regulation of ERBB2 by miR26a/b and HuR confers resistance to tamoxifen in estrogen receptor-positive breast cancer cells.

Authors:  Sheng Tan; Keshuo Ding; Qing-Yun Chong; Junsong Zhao; Yuan Liu; Yunying Shao; Yuanyuan Zhang; Qing Yu; Zirui Xiong; Weijie Zhang; Min Zhang; Gaopeng Li; Xiaoni Li; Xiangjun Kong; Akhlaq Ahmad; Zhengsheng Wu; Qiang Wu; Xiaodong Zhao; Peter E Lobie; Tao Zhu
Journal:  J Biol Chem       Date:  2017-06-21       Impact factor: 5.157

6.  Systemic Analysis of miRNAs in PD Stress Condition: miR-5701 Modulates Mitochondrial-Lysosomal Cross Talk to Regulate Neuronal Death.

Authors:  Paresh Prajapati; Lakshmi Sripada; Kritarth Singh; Milton Roy; Khyati Bhatelia; Pooja Dalwadi; Rajesh Singh
Journal:  Mol Neurobiol       Date:  2017-07-14       Impact factor: 5.590

Review 7.  Cytomegalovirus microRNAs.

Authors:  Lauren Hook; Meaghan Hancock; Igor Landais; Robert Grabski; William Britt; Jay A Nelson
Journal:  Curr Opin Virol       Date:  2014-04-23       Impact factor: 7.090

Review 8.  MicroRNAs in ovarian carcinomas.

Authors:  Neetu Dahiya; Patrice J Morin
Journal:  Endocr Relat Cancer       Date:  2010-01-29       Impact factor: 5.678

9.  GABAergic mechanisms regulated by miR-33 encode state-dependent fear.

Authors:  Vladimir Jovasevic; Kevin A Corcoran; Katherine Leaderbrand; Naoki Yamawaki; Anita L Guedea; Helen J Chen; Gordon M G Shepherd; Jelena Radulovic
Journal:  Nat Neurosci       Date:  2015-08-17       Impact factor: 24.884

10.  Tandem affinity purification of miRNA target mRNAs (TAP-Tar).

Authors:  Nora Nonne; Maya Ameyar-Zazoua; Mouloud Souidi; Annick Harel-Bellan
Journal:  Nucleic Acids Res       Date:  2009-12-02       Impact factor: 16.971
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