Douglas Costa Gontijo1, Maria Fernanda Alves do Nascimento1, Hugo Vianna Silva Rody2, Rodrigo Andrade Magalhães3, Luciano Ferreira Margalho4, Geraldo Célio Brandão5, Alaíde Braga de Oliveira6. 1. Departamento de Produtos Farmacêuticos, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, Belo Horizonte, MG, 31270-901, Brazil. 2. Escola Superior de Agricultura "Luiz de Queiroz", Departamento de Genética, Universidade de São Paulo, Av. Pádua Dias, 11, Piracicaba, SP, 13418-900, Brazil. 3. Departamento de Botânica, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, Belo Horizonte, MG, 31270-901, Brazil. 4. Instituto Federal do Pará, Av. Arterial 5-B, s/n, Icuí-Guajará, Ananindeua, PA, 67140-000, Brazil. 5. Escola de Farmácia, Universidade Federal de Ouro Preto, Morro Do Cruzeiro, s/n, Ouro Prêto, MG, 35400-000, Brazil. 6. Departamento de Produtos Farmacêuticos, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, Belo Horizonte, MG, 31270-901, Brazil. alaidebragaoliveira@gmail.com.
Abstract
INTRODUCTION: A great variety of bioactive natural products has been reported for different Palicourea and Psychotria species (Rubiaceae). However, few of them as well as few of species of these botanical genera have been evaluated for antiplasmodial activity. OBJECTIVE: To assess the antiplasmodial activity of 24 extracts from Palicourea and Psychotria genera, along with the targeted LC-MS metabolite profiling, as well as identification of the main metabolites in the bioactive extracts. METHODS: Twenty four ethanol and acid-base extracts from Palicourea and Psychotria genera collected in the Amazonia and Atlantic Forest, Brazil, were evaluated against chloroquine-resistant Plasmodium falciparum W2 strain by PfLDH. The metabolite profiling and putative identification of metabolites from bioactive extracts were determined by LC-DAD-ESI-MS and LC-HRMS, respectively. RESULTS: The ethanol extracts disclosed low antiplasmodial activity (% GI < 50%). High antiplasmodial effect was observed for the acid-base extracts from Psychotria apoda and Psychotria colorata with 100% inhibition of parasite growth inhibition. Fragment ions related to pyrrolidinoindoline alkaloids were observed by LC-DAD-ESI-MS mainly in the most bioactive extracts. The results of the in vitro screening associated with the LC-DAD-ESI-MS and LC-HRMSn data allowed to predict, for the first time, the pyrrolidinoindoline alkaloids as possible antiplasmodial representing, then, new potential natural antimalarial hits. In addition, other metabolite classes such as flavanones, lignans and chalcones were also putatively identified in the bioactive extracts of Psychotria apoda, Psychotria capitata, and Psychotria poeppigiana. CONCLUSION: The present results point to Palicourea and Psychotria species as sources of new antimalarial hits.
INTRODUCTION: A great variety of bioactive natural products has been reported for different Palicourea and Psychotria species (Rubiaceae). However, few of them as well as few of species of these botanical genera have been evaluated for antiplasmodial activity. OBJECTIVE: To assess the antiplasmodial activity of 24 extracts from Palicourea and Psychotria genera, along with the targeted LC-MS metabolite profiling, as well as identification of the main metabolites in the bioactive extracts. METHODS: Twenty four ethanol and acid-base extracts from Palicourea and Psychotria genera collected in the Amazonia and Atlantic Forest, Brazil, were evaluated against chloroquine-resistant Plasmodium falciparum W2 strain by PfLDH. The metabolite profiling and putative identification of metabolites from bioactive extracts were determined by LC-DAD-ESI-MS and LC-HRMS, respectively. RESULTS: The ethanol extracts disclosed low antiplasmodial activity (% GI < 50%). High antiplasmodial effect was observed for the acid-base extracts from Psychotria apoda and Psychotria colorata with 100% inhibition of parasite growth inhibition. Fragment ions related to pyrrolidinoindoline alkaloids were observed by LC-DAD-ESI-MS mainly in the most bioactive extracts. The results of the in vitro screening associated with the LC-DAD-ESI-MS and LC-HRMSn data allowed to predict, for the first time, the pyrrolidinoindoline alkaloids as possible antiplasmodial representing, then, new potential natural antimalarial hits. In addition, other metabolite classes such as flavanones, lignans and chalcones were also putatively identified in the bioactive extracts of Psychotria apoda, Psychotria capitata, and Psychotria poeppigiana. CONCLUSION: The present results point to Palicourea and Psychotria species as sources of new antimalarial hits.
Authors: Douglas Costa Gontijo; Geraldo Célio Brandão; Pablo Costa Gontijo; Alaíde Braga de Oliveira; Marisa Alves Nogueira Diaz; Luciano Gomes Fietto; João Paulo Viana Leite Journal: Food Chem Date: 2017-03-18 Impact factor: 7.514
Authors: Andreas Berger; Maria Katharina Kostyan; Simon Immo Klose; Michael Gastegger; Eberhard Lorbeer; Lothar Brecker; Johann Schinnerl Journal: Phytochemistry Date: 2015-06-01 Impact factor: 4.072
Authors: Namki Cho; Ana Lisa Valenciano; Yongle Du; Jason Clement; Maria B Cassera; Michael Goetz; David G I Kingston Journal: Bioorg Med Chem Lett Date: 2018-09-04 Impact factor: 2.823
Authors: Andreas Berger; Elias Tanuhadi; Lothar Brecker; Johann Schinnerl; Karin Valant-Vetschera Journal: Phytochemistry Date: 2017-08-12 Impact factor: 4.072