Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Structural basis of target DNA recognition by CRISPR-Cas12k for RNA-guided DNA transposition.

Literature DB >> 34450043

Structural basis of target DNA recognition by CRISPR-Cas12k for RNA-guided DNA transposition.

Renjian Xiao¹, Shukun Wang¹, Ruijie Han¹, Zhuang Li¹, Clinton Gabel¹, Indranil Arun Mukherjee¹, Leifu Chang².

Abstract

The type V-K CRISPR-Cas system, featured by Cas12k effector with a naturally inactivated RuvC domain and associated with Tn7-like transposon for RNA-guided DNA transposition, is a promising tool for precise DNA insertion. To reveal the mechanism underlying target DNA recognition, we determined a cryoelectron microscopy (cryo-EM) structure of Cas12k from cyanobacteria Scytonema hofmanni in complex with a single guide RNA (sgRNA) and a double-stranded target DNA. Coupled with mutagenesis and in vitro DNA transposition assay, our results revealed mechanisms for the recognition of the GGTT protospacer adjacent motif (PAM) sequence and the structural elements of Cas12k critical for RNA-guided DNA transposition. These structural and mechanistic insights should aid in the development of type V-K CRISPR-transposon systems as tools for genome editing.

Entities: Chemical

Keywords: CAST; CRISPR; Cas12k; cryo-EM; genome editing; transposon

Mesh：

Substances：
RNA, Guide
RNA
DNA

Year: 2021 PMID： 34450043 PMCID： PMC8571069 DOI： 10.1016/j.molcel.2021.07.043

Source DB: PubMed Journal: Mol Cell ISSN： 1097-2765 Impact factor: 17.970

62 in total

1. The sequence manipulation suite: JavaScript programs for analyzing and formatting protein and DNA sequences.

Authors: P Stothard
Journal: Biotechniques Date: 2000-06 Impact factor: 1.993

2. Functionally diverse type V CRISPR-Cas systems.

Authors: Winston X Yan; Pratyusha Hunnewell; Lauren E Alfonse; Jason M Carte; Elise Keston-Smith; Shanmugapriya Sothiselvam; Anthony J Garrity; Shaorong Chong; Kira S Makarova; Eugene V Koonin; David R Cheng; David A Scott
Journal: Science Date: 2018-12-06 Impact factor: 47.728

3. Structure of the miniature type V-F CRISPR-Cas effector enzyme.

Authors: Satoru N Takeda; Ryoya Nakagawa; Sae Okazaki; Hisato Hirano; Kan Kobayashi; Tsukasa Kusakizako; Tomohiro Nishizawa; Keitaro Yamashita; Hiroshi Nishimasu; Osamu Nureki
Journal: Mol Cell Date: 2020-12-16 Impact factor: 17.970

4. Structural Basis for Guide RNA Processing and Seed-Dependent DNA Targeting by CRISPR-Cas12a.

Authors: Daan C Swarts; John van der Oost; Martin Jinek
Journal: Mol Cell Date: 2017-04-20 Impact factor: 17.970

5. Comment on "RNA-guided DNA insertion with CRISPR-associated transposases".

Authors: Phoebe A Rice; Nancy L Craig; Fred Dyda
Journal: Science Date: 2020-06-05 Impact factor: 47.728

6. Structural Basis for the Altered PAM Recognition by Engineered CRISPR-Cpf1.

Authors: Hiroshi Nishimasu; Takashi Yamano; Linyi Gao; Feng Zhang; Ryuichiro Ishitani; Osamu Nureki
Journal: Mol Cell Date: 2017-06-06 Impact factor: 17.970

Review 7. An updated evolutionary classification of CRISPR-Cas systems.

Authors: Kira S Makarova; Yuri I Wolf; Omer S Alkhnbashi; Fabrizio Costa; Shiraz A Shah; Sita J Saunders; Rodolphe Barrangou; Stan J J Brouns; Emmanuelle Charpentier; Daniel H Haft; Philippe Horvath; Sylvain Moineau; Francisco J M Mojica; Rebecca M Terns; Michael P Terns; Malcolm F White; Alexander F Yakunin; Roger A Garrett; John van der Oost; Rolf Backofen; Eugene V Koonin
Journal: Nat Rev Microbiol Date: 2015-09-28 Impact factor: 60.633

Structural basis of target DNA recognition by CRISPR-Cas12k for RNA-guided DNA transposition.

1. The sequence manipulation suite: JavaScript programs for analyzing and formatting protein and DNA sequences.

2. Functionally diverse type V CRISPR-Cas systems.

3. Structure of the miniature type V-F CRISPR-Cas effector enzyme.

4. Structural Basis for Guide RNA Processing and Seed-Dependent DNA Targeting by CRISPR-Cas12a.

5. Comment on "RNA-guided DNA insertion with CRISPR-associated transposases".

6. Structural Basis for the Altered PAM Recognition by Engineered CRISPR-Cpf1.

Review 7. An updated evolutionary classification of CRISPR-Cas systems.

8. Mechanisms for target recognition and cleavage by the Cas12i RNA-guided endonuclease.

9. The μ transpososome structure sheds light on DDE recombinase evolution.

Review 1. Structural biology of CRISPR-Cas immunity and genome editing enzymes.

Review 2. CRISPR-based genome editing through the lens of DNA repair.

3. Mechanistic details of CRISPR-associated transposon recruitment and integration revealed by cryo-EM.

4. CRISPRtracrRNA: robust approach for CRISPR tracrRNA detection.

5. Structure of the TnsB transposase-DNA complex of type V-K CRISPR-associated transposon.