| Literature DB >> 3443615 |
A Sano1, S Matsutani, M Suzuki, S Takitani.
Abstract
The method described here is based on a separation of deoxynivalenol, nivalenol and fusarenon-X on a C18 column using aqueous acetonitrile, and successive post-column fluorescence derivatization involving an alkaline decomposition to form formaldehyde and modified Hantzsch reaction with methyl acetoacetate and ammonium acetate (lambda ex = 370 nm and lambda em = 460 nm). By this method, 5-10 ng of the standard trichothecenes could be determined. By employing a clean-up procedure with a florisil column and a Sep-Pak CN cartridge, 61.4-96.9% recoveries were obtained for deoxynivalenol and nivalenol added to corn, wheat and barley at concentration levels of 0.05-1 ppm.Entities:
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Year: 1987 PMID： 3443615 DOI： 10.1016/s0021-9673(00)90072-4
Source DB: PubMed Journal: J Chromatogr