Literature DB >> 3442819

Molecular cloning of a ribosomal protein gene from the fission yeast Schizosaccharomyces pombe.

R Nischt1, E Thüroff, N F Küfer.   

Abstract

Using the structural gene for the ribosomal protein L3 from Saccharomyces cerevisiae as a probe, we isolated a homologous fragment from genomic DNA of Schizosaccharomyces pombe. Analysis of the plasmid carrying this fragment by hybridization selection and 2D-electrophoresis revealed a 31 kDa ribosomal protein. Transformation of the vector pDB248x containing this fragment into Schizosaccharomyces pombe leads to an increased level of mRNA suggesting that we have cloned the entire and actively transcribed gene.

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Year:  1986        PMID： 3442819     DOI： 10.1007/BF00418408

Source DB:  PubMed          Journal:  Curr Genet        ISSN： 0172-8083            Impact factor:   3.886


  18 in total

1.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

2.  The ribosomal proteins of Saccharomyces cerevisiae.

Authors:  J R Warner; C Gorenstein
Journal:  Methods Cell Biol       Date:  1978       Impact factor: 1.441

3.  The use of R-looping for structural gene identification and mRNA purification.

Authors:  J L Woolford; M Rosbash
Journal:  Nucleic Acids Res       Date:  1979-06-11       Impact factor: 16.971

4.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

5.  The structure of the gene coding for the phosphorylated ribosomal protein S10 in yeast.

Authors:  R J Leer; M M van Raamsdonk-Duin; C M Molenaar; L H Cohen; W H Mager; R J Planta
Journal:  Nucleic Acids Res       Date:  1982-10-11       Impact factor: 16.971

6.  Collection of published 5S and 5.8S ribosomal RNA sequences.

Authors:  V A Erdmann; E Huysmans; A Vandenberghe; R De Wachter
Journal:  Nucleic Acids Res       Date:  1983-01-11       Impact factor: 16.971

7.  Characterisation of the ribosomal proteins from Schizosaccharomyces pombe by two-dimensional polyacrylamide gel electrophoresis: demonstration that a cycloheximide resistant strain, cyh1, has an altered 60S ribosomal protein.

Authors:  A Coddington; R Fluri
Journal:  Mol Gen Genet       Date:  1977-12-14

8.  The gene family encoding the mouse ribosomal protein L32 contains a uniquely expressed intron-containing gene and an unmutated processed gene.

Authors:  K P Dudov; R P Perry
Journal:  Cell       Date:  1984-06       Impact factor: 41.582

9.  Yeast ribosomal proteins: VII. Cytoplasmic ribosomal proteins from Schizosaccharomyces pombe.

Authors:  E Otaka; K Higo; T Itoh
Journal:  Mol Gen Genet       Date:  1983

10.  Nucleotide sequence of the tcml gene (ribosomal protein L3) of Saccharomyces cerevisiae.

Authors:  L D Schultz; J D Friesen
Journal:  J Bacteriol       Date:  1983-07       Impact factor: 3.490
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  19 in total

1.  The protein sequence and some intron positions are conserved between the switching gene swi10 of Schizosaccharomyces pombe and the human excision repair gene ERCC1.

Authors:  C Rödel; S Kirchhoff; H Schmidt
Journal:  Nucleic Acids Res       Date:  1992-12-11       Impact factor: 16.971

2.  prp4 from Schizosaccharomyces pombe, a mutant deficient in pre-mRNA splicing isolated using genes containing artificial introns.

Authors:  G H Rosenberg; S K Alahari; N F Käufer
Journal:  Mol Gen Genet       Date:  1991-04

3.  spp42, identified as a classical suppressor of prp4-73, which encodes a kinase involved in pre-mRNA splicing in fission yeast, is a homologue of the splicing factor Prp8p.

Authors:  H Schmidt; K Richert; R A Drakas; N F Käufer
Journal:  Genetics       Date:  1999-11       Impact factor: 4.562

4.  Immunological homologies between yeast ribosomal protein L2 and rat liver ribosomal proteins L4 and L24.

Authors:  C Kreutzfeldt; M Potthast
Journal:  Curr Genet       Date:  1988-03       Impact factor: 3.886

5.  Primary structure of the ribosomal protein gene S6 from Schizosaccharomyces pombe.

Authors:  T Gross; R Nischt; K Gatermann; U Swida; N F Käufer
Journal:  Curr Genet       Date:  1988       Impact factor: 3.886

6.  Photoactive mitochondria: in vivo transfer of a light-driven proton pump into the inner mitochondrial membrane of Schizosaccharomyces pombe.

Authors:  A Hoffmann; V Hildebrandt; J Heberle; G Büldt
Journal:  Proc Natl Acad Sci U S A       Date:  1994-09-27       Impact factor: 11.205

7.  Sequence and regulatory responses of a ribosomal protein gene from the fission yeast Schizosaccharomyces pombe.

Authors:  R Nischt; T Gross; K Gatermann; U Swida; N Käufer
Journal:  Nucleic Acids Res       Date:  1987-02-25       Impact factor: 16.971

8.  Characterisation of Saccharomyces cerevisiae genes encoding ribosomal protein YL6.

Authors:  J Moore; H T Jacobs; K Kaiser
Journal:  Mol Gen Genet       Date:  1995-04-20

9.  Introduction of functional artificial introns into the naturally intronless ura4 gene of Schizosaccharomyces pombe.

Authors:  K B Gatermann; A Hoffmann; G H Rosenberg; N F Käufer
Journal:  Mol Cell Biol       Date:  1989-04       Impact factor: 4.272

10.  Schizosaccharomyces U6 genes have a sequence within their introns that matches the B box consensus of tRNA internal promoters.

Authors:  D Frendewey; I Barta; M Gillespie; J Potashkin
Journal:  Nucleic Acids Res       Date:  1990-04-25       Impact factor: 16.971
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