Literature DB >> 379820

The use of R-looping for structural gene identification and mRNA purification.

J L Woolford, M Rosbash.   

Abstract

A method is presented for the purification of mRNAs and the identification of structural gene sequences in recombinant DNA molecules. RNA is hybridized to double-stranded linear DNA such that R-loops are formed between most DNAs and their complementary RNA sequences. These R-loops are purified from unhybridized RNAs by gel filtration chromatography in the presence of a high concentration of salt. The complementary RNAs are released from the R-loops by heating, and are assayed by gel electrophoresis or cell free translation to determine their purity and to identify the proteins for which they code. We have demonstrated that recombinant DNAs containing sequences for abundant or moderately abundant mRNAs of Saccharomyces cerevisiae can be identified by this means.

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Year:  1979        PMID： 379820      PMCID： PMC327867          DOI： 10.1093/nar/6.7.2483

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  24 in total

1.  Rates of formation and thermal stabilities of RNA:DNA and DNA:DNA duplexes at high concentrations of formamide.

Authors:  J Casey; N Davidson
Journal:  Nucleic Acids Res       Date:  1977       Impact factor: 16.971

2.  SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA.

Authors:  F W STUDIER
Journal:  J Mol Biol       Date:  1965-02       Impact factor: 5.469

3.  Analysis of mRNA populations by cDNA.mRNA hybrid-mediated inhibition of cell-free protein synthesis.

Authors:  N D Hastie; W A Held
Journal:  Proc Natl Acad Sci U S A       Date:  1978-03       Impact factor: 11.205

4.  Isolation and analysis of recombinant DNA molecules containing yeast DNA.

Authors:  T D Petes; J R Broach; P C Wensink; L M Hereford; G R Fink; D Botstein
Journal:  Gene       Date:  1978-09       Impact factor: 3.688

5.  Regulation of a set of abundant mRNA sequences.

Authors:  L M Hereford; M Rosbash
Journal:  Cell       Date:  1977-03       Impact factor: 41.582

6.  Positions of sea urchin (Strongylocentrotus purpuratus) histone genes relative to restriction endonuclease sites on the chimeric plasmids pSp2 and pSp17.

Authors:  D S Holmes; R H Cohn; L H Kedes; N Davidson
Journal:  Biochemistry       Date:  1977-04-05       Impact factor: 3.162

7.  Messenger RNA in heat-shocked Drosophila cells.

Authors:  A Spradling; M L Pardue; S Penman
Journal:  J Mol Biol       Date:  1977-02-05       Impact factor: 5.469

8.  Functional expression of cloned yeast DNA in Escherichia coli.

Authors:  B Ratzkin; J Carbon
Journal:  Proc Natl Acad Sci U S A       Date:  1977-02       Impact factor: 11.205

9.  R loop mapping of the 18S and 28S sequences in the long and short repeating units of Drosophila melanogaster rDNA.

Authors:  R L White; D S Hogness
Journal:  Cell       Date:  1977-02       Impact factor: 41.582

10.  Spliced segments at the 5' terminus of adenovirus 2 late mRNA.

Authors:  S M Berget; C Moore; P A Sharp
Journal:  Proc Natl Acad Sci U S A       Date:  1977-08       Impact factor: 11.205
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  30 in total

1.  Filter replicas and permanent collections of recombinant DNA plasmids.

Authors:  J P Gergen; R H Stern; P C Wensink
Journal:  Nucleic Acids Res       Date:  1979-12-20       Impact factor: 16.971

2.  Cloned DNA sequences complementary to mRNAs encoding precursors to the small subunit of ribulose-1,5-bisphosphate carboxylase and a chlorophyll a/b binding polypeptide.

Authors:  R Broglie; G Bellemare; S G Bartlett; N H Chua; A R Cashmore
Journal:  Proc Natl Acad Sci U S A       Date:  1981-12       Impact factor: 11.205

3.  Identification and organization of carbon dioxide fixation genes in Xanthobacter flavus H4-14.

Authors:  W G Meijer; A C Arnberg; H G Enequist; P Terpstra; M E Lidstrom; L Dijkhuizen
Journal:  Mol Gen Genet       Date:  1991-02

4.  Characterization of the rDNA repeat units in the MitchellPetunia genome.

Authors:  J Waldron; P Dunsmuir; J Bedbrook
Journal:  Plant Mol Biol       Date:  1983-03       Impact factor: 4.076

5.  Localization of genes for coupling factor subunits on the spinach plastid chromosome.

Authors:  P Westhoff; N Nelson; H Bünemann; R G Herrmann
Journal:  Curr Genet       Date:  1981-11       Impact factor: 3.886

6.  Purification of the mRNA for chicken very low density lipoproteinII and molecular cloning of its full-length double-stranded cDNA.

Authors:  B Wieringa; W Roskam; A Arnberg; J van der Zwaag-Gerritsen; G Ab; M Gruber
Journal:  Nucleic Acids Res       Date:  1979-12-20       Impact factor: 16.971

7.  Kinetics of spontaneous displacement of RNA from heteroduplexes by DNA.

Authors:  R Landgraf; K S Ramamurthi; D S Sigman
Journal:  Nucleic Acids Res       Date:  1996-08-15       Impact factor: 16.971

8.  Anatomy of mitochondrial DNA from Paramecium aurelia.

Authors:  D J Cummings; R A Maki; P J Conlon; J Laping
Journal:  Mol Gen Genet       Date:  1980

9.  Sequence-specific scission of DNA by the chemical nuclease activity of 1,10-phenanthroline-copper(I) targeted by RNA.

Authors:  C B Chen; M B Gorin; D S Sigman
Journal:  Proc Natl Acad Sci U S A       Date:  1993-05-01       Impact factor: 11.205

10.  Hybridization-selected translation of Bombyx mori high-cysteine chorion proteins in Xenopus laevis oocytes.

Authors:  S C Bock; D C Tiemeier; K Mester; M Wu; M R Goldsmith
Journal:  Proc Natl Acad Sci U S A       Date:  1982-02       Impact factor: 11.205
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