Literature DB >> 34292547

Ni-NTA Affinity Chromatography to Characterize Protein-Protein Interactions During Fe-S Cluster Biogenesis.

Terrell D Carter1, F Wayne Outten2.   

Abstract

Affinity chromatography can be repurposed to provide useful information about the specific partner protein(s) to which a protein of interest may bind as well as the relative binding affinity of that partner protein for the protein of interest. Here, we provide a protocol for an Ni-NTA affinity chromatography assay that may be utilized to uncover insightful information about the nature of protein-protein interactions during iron-sulfur (Fe-S) cluster biogenesis reactions.
© 2021. Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Affinity chromatography; Anaerobic; IMAC; Iron-sulfur cluster

Mesh：

Substances：

Year:  2021        PMID： 34292547      PMCID： PMC8969215          DOI： 10.1007/978-1-0716-1605-5_7

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  9 in total

Review 1.  Iron-sulphur clusters and the problem with oxygen.

Authors:  James A Imlay
Journal:  Mol Microbiol       Date:  2006-02       Impact factor: 3.501

Review 2.  Immobilized-metal affinity chromatography (IMAC): a review.

Authors:  Helena Block; Barbara Maertens; Anne Spriestersbach; Nicole Brinker; Jan Kubicek; Roland Fabis; Jörg Labahn; Frank Schäfer
Journal:  Methods Enzymol       Date:  2009       Impact factor: 1.600

Review 3.  Fe-S cluster biogenesis by the bacterial Suf pathway.

Authors:  Matthew Blahut; Enis Sanchez; Claire E Fisher; F Wayne Outten
Journal:  Biochim Biophys Acta Mol Cell Res       Date:  2020-08-18       Impact factor: 4.739

Review 4.  Techniques to isolate O2-sensitive proteins: [4Fe-4S]-FNR as an example.

Authors:  Aixin Yan; Patricia J Kiley
Journal:  Methods Enzymol       Date:  2009       Impact factor: 1.600

5.  The SufBCD Fe-S scaffold complex interacts with SufA for Fe-S cluster transfer.

Authors:  Harsimranjit K Chahal; Yuyuan Dai; Avneesh Saini; Carla Ayala-Castro; F Wayne Outten
Journal:  Biochemistry       Date:  2009-11-10       Impact factor: 3.162

6.  Removal of dissolved oxygen from water: A comparison of four common techniques.

Authors:  I B Butler; M A Schoonen; D T Rickard
Journal:  Talanta       Date:  1994-02       Impact factor: 6.057

7.  Elevated Expression of a Functional Suf Pathway in Escherichia coli BL21(DE3) Enhances Recombinant Production of an Iron-Sulfur Cluster-Containing Protein.

Authors:  Elliot I Corless; Erin L Mettert; Patricia J Kiley; Edwin Antony
Journal:  J Bacteriol       Date:  2020-01-15       Impact factor: 3.490

Review 8.  Iron-sulfur cluster biosynthesis and trafficking - impact on human disease conditions.

Authors:  C Wachnowsky; I Fidai; J A Cowan
Journal:  Metallomics       Date:  2018-01-24       Impact factor: 4.526

9.  Biochemical Reconstitution and Spectroscopic Analysis of Iron-Sulfur Proteins.

Authors:  Sven-Andreas Freibert; Benjamin D Weiler; Eckhard Bill; Antonio J Pierik; Ulrich Mühlenhoff; Roland Lill
Journal:  Methods Enzymol       Date:  2018-01-10       Impact factor: 1.600
  9 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.