| Literature DB >> 19892187 |
Helena Block1, Barbara Maertens, Anne Spriestersbach, Nicole Brinker, Jan Kubicek, Roland Fabis, Jörg Labahn, Frank Schäfer.
Abstract
This article reviews the development of immobilized-metal affinity chromatography (IMAC) and describes its most important applications. We provide an overview on the use of IMAC in protein fractionation and proteomics, in protein immobilization and detection, and on some special applications such as purification of immunoglobulins and the Chelex method. The most relevant application-purification of histidine-tagged recombinant proteins-will be reviewed in greater detail with focus of state-of-the-art materials, methods, and protocols, and the limitations of IMAC and recent advances to improve the technology and the methods will be described.Entities:
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Year: 2009 PMID： 19892187 DOI： 10.1016/S0076-6879(09)63027-5
Source DB: PubMed Journal: Methods Enzymol ISSN： 0076-6879 Impact factor: 1.600