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Literature DB >> 34286039

Time-of-addition and Temperature-shift Assays to Determine Particular Step(s) in the Viral Life Cycle that is Blocked by Antiviral Substance(s).

Chie Aoki-Utsubo¹, Ming Chen², Hak Hotta^1,2,3.

Abstract

Viruses infect their host cells to produce progeny virus particles through the sequential steps of the viral life cycle, such as viral attachment, entry, penetration and post-entry events. This protocol describes time-of-addition and temperature-shift assays that are employed to explore which step(s) in the viral life cycle is blocked by an antiviral substance(s).

Entities: Chemical

Keywords: Attachment; Co-treatment; Penetration; Post-entry treatment; Pretreatment; Temperature-shift assay; Time-of-addition assay; Viral life cycle

Year: 2018 PMID： 34286039 PMCID： PMC8275244 DOI： 10.21769/BioProtoc.2830

Source DB: PubMed Journal: Bio Protoc ISSN： 2331-8325

10 in total

1. Guidelines for accurate EC50/IC50 estimation.

Authors: J L Sebaugh
Journal: Pharm Stat Date: 2011 Mar-Apr Impact factor: 1.894

Review 2. Understanding the hepatitis C virus life cycle paves the way for highly effective therapies.

Authors: Troels K H Scheel; Charles M Rice
Journal: Nat Med Date: 2013-07 Impact factor: 53.440

3. Secreted phospholipases A(2), a new class of HIV inhibitors that block virus entry into host cells.

Authors: D Fenard; G Lambeau; E Valentin; J C Lefebvre; M Lazdunski; A Doglio
Journal: J Clin Invest Date: 1999-09 Impact factor: 14.808

4. Antiviral activity of extracts from Morinda citrifolia leaves and chlorophyll catabolites, pheophorbide a and pyropheophorbide a, against hepatitis C virus.

Authors: Suratno Lulut Ratnoglik; Chie Aoki; Pratiwi Sudarmono; Mari Komoto; Lin Deng; Ikuo Shoji; Hiroyuki Fuchino; Nobuo Kawahara; Hak Hotta
Journal: Microbiol Immunol Date: 2014-03 Impact factor: 1.955

5. Anti-Hepatitis C Virus Activity of a Crude Extract from Longan (Dimocarpus longan Lour.) Leaves.

Authors: Dadan Ramadhan Apriyanto; Chie Aoki; Sri Hartati; Muhammad Hanafi; Leonardus Broto Sugeng Kardono; Ade Arsianti; Melva Louisa; Tjahjani Mirawati Sudiro; Beti Ernawati Dewi; Pratiwi Sudarmono; Amin Soebandrio; Hak Hotta
Journal: Jpn J Infect Dis Date: 2015-08-07 Impact factor: 1.362

6. Virocidal activity of Egyptian scorpion venoms against hepatitis C virus.

Authors: Alaa M H El-Bitar; Moustafa M H Sarhan; Chie Aoki; Yusuke Takahara; Mari Komoto; Lin Deng; Mohsen A Moustafa; Hak Hotta
Journal: Virol J Date: 2015-03-24 Impact factor: 4.099

7. Broad-spectrum antiviral agents: secreted phospholipase A₂ targets viral envelope lipid bilayers derived from the endoplasmic reticulum membrane.

Authors: Ming Chen; Chie Aoki-Utsubo; Masanori Kameoka; Lin Deng; Yutaka Terada; Wataru Kamitani; Kei Sato; Yoshio Koyanagi; Makoto Hijikata; Keiko Shindo; Takeshi Noda; Michinori Kohara; Hak Hotta
Journal: Sci Rep Date: 2017-11-21 Impact factor: 4.379

8. Anti-hepatitis C virus compounds obtained from Glycyrrhiza uralensis and other Glycyrrhiza species.

Authors: Myrna Adianti; Chie Aoki; Mari Komoto; Lin Deng; Ikuo Shoji; Tutik Sri Wahyuni; Maria Inge Lusida; Hiroyuki Fuchino; Nobuo Kawahara; Hak Hotta
Journal: Microbiol Immunol Date: 2014-03 Impact factor: 1.955

9. Immunofluorescent Staining of Mouse Intestinal Stem Cells.

Authors: Kevin P O'Rourke; Lukas E Dow; Scott W Lowe
Journal: Bio Protoc Date: 2016-02-20

10. Antiviral activities of Indonesian medicinal plants in the East Java region against hepatitis C virus.

Authors: Tutik Sri Wahyuni; Lydia Tumewu; Adita Ayu Permanasari; Evhy Apriani; Myrna Adianti; Abdul Rahman; Aty Widyawaruyanti; Maria Inge Lusida; Achmad Fuad; Hiroyuki Fuchino; Nobuo Kawahara; Ikuo Shoji; Lin Deng; Chie Aoki; Hak Hotta
Journal: Virol J Date: 2013-08-13 Impact factor: 4.099