Literature DB >> 27570798

Immunofluorescent Staining of Mouse Intestinal Stem Cells.

Kevin P O'Rourke1, Lukas E Dow2, Scott W Lowe3.   

Abstract

Immunofluorescent staining of organoids can be performed to visualize molecular markers of cell behavior. For example, cell proliferation marked by incorporation of nucleotide (EdU), or to observe markers of intestinal differentiation including paneth cells, goblet cells, or enterocytes (see Figure 1). In this protocol we detail a method to fix, permeabilize, stain and mount intestinal organoids for analysis by immunofluorescent confocal microscopy.

Entities:  

Year:  2016        PMID： 27570798      PMCID： PMC4996654          DOI： 10.21769/bioprotoc.1732

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  2 in total

1.  Apc Restoration Promotes Cellular Differentiation and Reestablishes Crypt Homeostasis in Colorectal Cancer.

Authors:  Lukas E Dow; Kevin P O'Rourke; Janelle Simon; Darjus F Tschaharganeh; Johan H van Es; Hans Clevers; Scott W Lowe
Journal:  Cell       Date:  2015-06-18       Impact factor: 41.582

2.  Isolation, Culture, and Maintenance of Mouse Intestinal Stem Cells.

Authors:  Kevin P O'Rourke; Sarah Ackerman; Lukas E Dow; Scott W Lowe
Journal:  Bio Protoc       Date:  2016-02-20
  2 in total
  12 in total

1.  Absence of neurotensin attenuates intestinal dysbiosis and inflammation by maintaining Mmp7/α-defensin axis in diet-induced obese mice.

Authors:  Jing Li; Xian Li; Jun Song; Baoxiang Yan; Stephanie A Rock; Jianhang Jia; Jinpeng Liu; Chi Wang; Todd Weiss; Heidi L Weiss; Tianyan Gao; Ashfaqul Alam; B Mark Evers
Journal:  FASEB J       Date:  2020-05-02       Impact factor: 5.191

2.  Distinct Colorectal Cancer-Associated APC Mutations Dictate Response to Tankyrase Inhibition.

Authors:  Emma M Schatoff; Sukanya Goswami; Maria Paz Zafra; Miguel Foronda; Michael Shusterman; Benjamin I Leach; Alyna Katti; Bianca J Diaz; Lukas E Dow
Journal:  Cancer Discov       Date:  2019-07-23       Impact factor: 39.397

3.  Dietary fructose improves intestinal cell survival and nutrient absorption.

Authors:  Samuel R Taylor; Shakti Ramsamooj; Roger J Liang; Alyna Katti; Rita Pozovskiy; Neil Vasan; Seo-Kyoung Hwang; Navid Nahiyaan; Nancy J Francoeur; Emma M Schatoff; Jared L Johnson; Manish A Shah; Andrew J Dannenberg; Robert P Sebra; Lukas E Dow; Lewis C Cantley; Kyu Y Rhee; Marcus D Goncalves
Journal:  Nature       Date:  2021-08-18       Impact factor: 69.504

4.  Senescent Tumor Cells in the Peritoneal Carcinomatosis Drive Immunosenescence in the Tumor Microenvironment.

Authors:  Heidi Braumüller; Bernhard Mauerer; Christopher Berlin; Dorothea Plundrich; Patrick Marbach; Pierre Cauchy; Claudia Laessle; Esther Biesel; Philipp Anton Holzner; Rebecca Kesselring
Journal:  Front Immunol       Date:  2022-06-30       Impact factor: 8.786

5.  Time-of-addition and Temperature-shift Assays to Determine Particular Step(s) in the Viral Life Cycle that is Blocked by Antiviral Substance(s).

Authors:  Chie Aoki-Utsubo; Ming Chen; Hak Hotta
Journal:  Bio Protoc       Date:  2018-05-05

6.  Media from macrophages co-incubated with Enterococcus faecalis induces epithelial cell monolayer reassembly and altered cell morphology.

Authors:  Natalia Belogortseva; Monika Krezalek; Kristina Guyton; Christine Labno; Valeriy Poroyko; Olga Zaborina; John C Alverdy
Journal:  PLoS One       Date:  2017-08-09       Impact factor: 3.240

7.  Krüppel-like Factor 5 Regulates Stemness, Lineage Specification, and Regeneration of Intestinal Epithelial Stem Cells.

Authors:  Chang-Kyung Kim; Madhurima Saxena; Kasmika Maharjan; Jane J Song; Kenneth R Shroyer; Agnieszka B Bialkowska; Ramesh A Shivdasani; Vincent W Yang
Journal:  Cell Mol Gastroenterol Hepatol       Date:  2019-11-25

8.  Gut Microbiota-Derived Propionate Regulates the Expression of Reg3 Mucosal Lectins and Ameliorates Experimental Colitis in Mice.

Authors:  Danica Bajic; Adrian Niemann; Anna-Katharina Hillmer; Raquel Mejias-Luque; Sena Bluemel; Melissa Docampo; Maja C Funk; Elena Tonin; Michael Boutros; Bernd Schnabl; Dirk H Busch; Tsuyoshi Miki; Roland M Schmid; Marcel R M van den Brink; Markus Gerhard; Christoph K Stein-Thoeringer
Journal:  J Crohns Colitis       Date:  2020-10-05       Impact factor: 9.071

9.  Lineage Reversion Drives WNT Independence in Intestinal Cancer.

Authors:  Teng Han; Sukanya Goswami; Yang Hu; Fanying Tang; Maria Paz Zafra; Charles Murphy; Zhen Cao; John T Poirier; Ekta Khurana; Olivier Elemento; Jaclyn F Hechtman; Karuna Ganesh; Rona Yaeger; Lukas E Dow
Journal:  Cancer Discov       Date:  2020-06-16       Impact factor: 38.272

10.  Rfx6 promotes the differentiation of peptide-secreting enteroendocrine cells while repressing genetic programs controlling serotonin production.

Authors:  Julie Piccand; Constance Vagne; Florence Blot; Aline Meunier; Anthony Beucher; Perrine Strasser; Mari L Lund; Sabitri Ghimire; Laure Nivlet; Céline Lapp; Natalia Petersen; Maja S Engelstoft; Christelle Thibault-Carpentier; Céline Keime; Sara Jimenez Correa; Valérie Schreiber; Nacho Molina; Thue W Schwartz; Adèle De Arcangelis; Gérard Gradwohl
Journal:  Mol Metab       Date:  2019-08-13       Impact factor: 7.422
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