Literature DB >> 3426222

Isolation of intact high-molecular-weight DNA by using guanidine isothiocyanate.

J A Lippke1, M N Strzempko, F F Raia, S L Simon, C K French.   

Abstract

A method for obtaining high-molecular-weight chromosomal DNA from Bacteroides intermedius and Bacteroides gingivalis is described. This technique is a modification of the guanidine isothiocyanate isolation procedure for RNA and should be useful for isolating intact DNA from organisms with high endogenous nuclease activity.

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Year:  1987        PMID： 3426222      PMCID： PMC204150          DOI： 10.1128/aem.53.10.2588-2589.1987

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  1 in total

1.  Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.

Authors:  J M Chirgwin; A E Przybyla; R J MacDonald; W J Rutter
Journal:  Biochemistry       Date:  1979-11-27       Impact factor: 3.162
  1 in total
  14 in total

1.  The Ti plasmid of Agrobacterium tumefaciens harbors an attM-paralogous gene, aiiB, also encoding N-Acyl homoserine lactonase activity.

Authors:  A Carlier; S Uroz; B Smadja; R Fray; X Latour; Y Dessaux; D Faure
Journal:  Appl Environ Microbiol       Date:  2003-08       Impact factor: 4.792

2.  Polymerase chain reaction identification of Vibrio vulnificus in artificially contaminated oysters.

Authors:  W E Hill; S P Keasler; M W Trucksess; P Feng; C A Kaysner; K A Lampel
Journal:  Appl Environ Microbiol       Date:  1991-03       Impact factor: 4.792

3.  DNA-DNA Solution Hybridization Studies of the Bacterial Symbionts of Hydrothermal Vent Tube Worms (Riftia pachyptila and Tevnia jerichonana).

Authors:  D B Edwards; D C Nelson
Journal:  Appl Environ Microbiol       Date:  1991-04       Impact factor: 4.792

4.  Cloning and sequencing of a form II ribulose-1,5-biphosphate carboxylase/oxygenase from the bacterial symbiont of the hydrothermal vent tubeworm Riftia pachyptila.

Authors:  J J Robinson; J L Stein; C M Cavanaugh
Journal:  J Bacteriol       Date:  1998-03       Impact factor: 3.490

5.  Nucleotide sequence and expression of a deep-sea ribulose-1,5-bisphosphate carboxylase gene cloned from a chemoautotrophic bacterial endosymbiont.

Authors:  J L Stein; M Haygood; H Felbeck
Journal:  Proc Natl Acad Sci U S A       Date:  1990-11       Impact factor: 11.205

6.  Identification of a streptogramin A acetyltransferase gene in the chromosome of Yersinia enterocolitica.

Authors:  A Seoane; J M García Lobo
Journal:  Antimicrob Agents Chemother       Date:  2000-04       Impact factor: 5.191

7.  Rapid viability loss on exposure to air in a superoxide dismutase-deficient mutant of Porphyromonas gingivalis.

Authors:  K Nakayama
Journal:  J Bacteriol       Date:  1994-04       Impact factor: 3.490

8.  An effective method to extract DNA from environmental samples for polymerase chain reaction amplification and DNA fingerprint analysis.

Authors:  L A Porteous; J L Armstrong; R J Seidler; L S Watrud
Journal:  Curr Microbiol       Date:  1994-11       Impact factor: 2.188

9.  Isolation and characterization of a regulatory gene affecting rhamnolipid biosurfactant synthesis in Pseudomonas aeruginosa.

Authors:  U A Ochsner; A K Koch; A Fiechter; J Reiser
Journal:  J Bacteriol       Date:  1994-04       Impact factor: 3.490

10.  Involvement of arginine-specific cysteine proteinase (Arg-gingipain) in fimbriation of Porphyromonas gingivalis.

Authors:  K Nakayama; F Yoshimura; T Kadowaki; K Yamamoto
Journal:  J Bacteriol       Date:  1996-05       Impact factor: 3.490
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