Literature DB >> 3407910

A dye release assay for determination of lysostaphin activity.

R Zhou1, S Chen, P Recsei.   

Abstract

We describe a method for determination of lysostaphin activity using Remazol Brilliant Blue R (RBB)-dyed staphylococcal cells or RBB-dyed staphylococcal peptidoglycan as substrate. The dyed substrates are easy to prepare and are stable for at least 6 months. Soluble hydrolytic products released by lysostaphin are measured spectrophotometrically at 595 nm after the insoluble substrate is removed by filtration or centrifugation. The dye release assay is more sensitive and more accurate than the previously described turbidimetric assay.

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Year:  1988        PMID： 3407910     DOI： 10.1016/0003-2697(88)90134-0

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  28 in total

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4.  Crystal Structures of the SpoIID Lytic Transglycosylases Essential for Bacterial Sporulation.

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Journal:  J Biol Chem       Date:  2016-05-18       Impact factor: 5.157

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Authors:  Truc Do; Julia E Page; Suzanne Walker
Journal:  J Biol Chem       Date:  2020-01-23       Impact factor: 5.157

6.  Spectrophotometric detection of bacteriolytic activity of diluted lysostaphin solutions.

Authors:  I Márová; J Kovár
Journal:  Folia Microbiol (Praha)       Date:  1993       Impact factor: 2.099

7.  The bacterial septal ring protein RlpA is a lytic transglycosylase that contributes to rod shape and daughter cell separation in Pseudomonas aeruginosa.

Authors:  Matthew A Jorgenson; Yan Chen; Atsushi Yahashiri; David L Popham; David S Weiss
Journal:  Mol Microbiol       Date:  2014-05-23       Impact factor: 3.501

8.  The Gonococcal NlpD Protein Facilitates Cell Separation by Activating Peptidoglycan Cleavage by AmiC.

Authors:  Elizabeth A Stohl; Jonathan D Lenz; Joseph P Dillard; H Steven Seifert
Journal:  J Bacteriol       Date:  2015-11-16       Impact factor: 3.490

9.  Assessing the influence of adsorbed-state conformation on the bioactivity of adsorbed enzyme layers.

Authors:  Kenan P Fears; Robert A Latour
Journal:  Langmuir       Date:  2009-12-15       Impact factor: 3.882

10.  Probing the conformation and orientation of adsorbed enzymes using side-chain modification.

Authors:  Kenan P Fears; Balakrishnan Sivaraman; Gary L Powell; Yonnie Wu; Robert A Latour
Journal:  Langmuir       Date:  2009-08-18       Impact factor: 3.882
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