Literature DB >> 34062386

Gold nanoparticles and tilt pairs to assess protein flexibility by cryo-electron microscopy.

Milind Jagota1, Raphael J L Townshend2, Lin-Woo Kang3, David A Bushnell4, Ron O Dror5, Roger D Kornberg4, Maia Azubel6.   

Abstract

A computational method was developed to recover the three-dimensional coordinates of gold nanoparticles specifically attached to a protein complex from tilt-pair images collected by electron microscopy. The program was tested on a simulated dataset and applied to a real dataset comprising tilt-pair images recorded by cryo electron microscopy of RNA polymerase II in a complex with four gold-labeled single-chain antibody fragments. The positions of the gold nanoparticles were determined, and comparison of the coordinates among the tetrameric particles revealed the range of motion within the protein complexes. Published by Elsevier B.V.

Entities:  

Keywords:  Cryo-electron microscopy; Gold nanoparticles; Least-squares method; Single-chain Fv antibody; Tilt-pairs

Mesh：

Substances：

Year:  2021        PMID： 34062386      PMCID： PMC8238918          DOI： 10.1016/j.ultramic.2021.113302

Source DB:  PubMed          Journal:  Ultramicroscopy        ISSN： 0304-3991            Impact factor:   2.994


  27 in total

1.  Optimal determination of particle orientation, absolute hand, and contrast loss in single-particle electron cryomicroscopy.

Authors:  Peter B Rosenthal; Richard Henderson
Journal:  J Mol Biol       Date:  2003-10-31       Impact factor: 5.469

2.  EMAN2: an extensible image processing suite for electron microscopy.

Authors:  Guang Tang; Liwei Peng; Philip R Baldwin; Deepinder S Mann; Wen Jiang; Ian Rees; Steven J Ludtke
Journal:  J Struct Biol       Date:  2006-06-08       Impact factor: 2.867

3.  Assembly of combinatorial antibody libraries on phage surfaces: the gene III site.

Authors:  C F Barbas; A S Kang; R A Lerner; S J Benkovic
Journal:  Proc Natl Acad Sci U S A       Date:  1991-09-15       Impact factor: 11.205

4.  Image formation modeling in cryo-electron microscopy.

Authors:  Miloš Vulović; Raimond B G Ravelli; Lucas J van Vliet; Abraham J Koster; Ivan Lazić; Uwe Lücken; Hans Rullgård; Ozan Öktem; Bernd Rieger
Journal:  J Struct Biol       Date:  2013-05-25       Impact factor: 2.867

Review 5.  Cryo-Electron Microscopy Methodology: Current Aspects and Future Directions.

Authors:  Radostin Danev; Haruaki Yanagisawa; Masahide Kikkawa
Journal:  Trends Biochem Sci       Date:  2019-05-08       Impact factor: 13.807

6.  Yeast surface display for screening combinatorial polypeptide libraries.

Authors:  E T Boder; K D Wittrup
Journal:  Nat Biotechnol       Date:  1997-06       Impact factor: 54.908

7.  Structure of a thiol monolayer-protected gold nanoparticle at 1.1 A resolution.

Authors:  Pablo D Jadzinsky; Guillermo Calero; Christopher J Ackerson; David A Bushnell; Roger D Kornberg
Journal:  Science       Date:  2007-10-19       Impact factor: 47.728

Review 8.  Single-particle cryo-EM-How did it get here and where will it go.

Authors:  Yifan Cheng
Journal:  Science       Date:  2018-08-31       Impact factor: 47.728

Review 9.  Unravelling biological macromolecules with cryo-electron microscopy.

Authors:  Rafael Fernandez-Leiro; Sjors H W Scheres
Journal:  Nature       Date:  2016-09-15       Impact factor: 49.962

10.  Pre-pro is a fast pre-processor for single-particle cryo-EM by enhancing 2D classification.

Authors:  Szu-Chi Chung; Hsin-Hung Lin; Po-Yao Niu; Shih-Hsin Huang; I-Ping Tu; Wei-Hau Chang
Journal:  Commun Biol       Date:  2020-09-11
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.