Literature DB >> 1896445

Assembly of combinatorial antibody libraries on phage surfaces: the gene III site.

C F Barbas1, A S Kang, R A Lerner, S J Benkovic.   

Abstract

A phagemid system was developed for the monovalent display of combinatorial antibody Fab libraries on the surface of filamentous phage M13. Fab fragments were fused to the carboxyl-terminal domain of the gene III protein. Phage displaying Fab fragments on their surface, or Phabs, were enriched by 10(3)- to 10(5)-fold on antigen-coated surfaces over nonspecific phage. The method may replace current antibody cloning techniques.

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Year:  1991        PMID： 1896445      PMCID： PMC52428          DOI： 10.1073/pnas.88.18.7978

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  23 in total

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Authors:  A D Ellington; J W Szostak
Journal:  Nature       Date:  1990-08-30       Impact factor: 49.962

2.  Searching for peptide ligands with an epitope library.

Authors:  J K Scott; G P Smith
Journal:  Science       Date:  1990-07-27       Impact factor: 47.728

3.  Hormone phage: an enrichment method for variant proteins with altered binding properties.

Authors:  S Bass; R Greene; J A Wells
Journal:  Proteins       Date:  1990

4.  Identification of clones that encode chicken tropomyosin by direct immunological screening of a cDNA expression library.

Authors:  D M Helfman; J R Feramisco; J C Fiddes; G P Thomas; S H Hughes
Journal:  Proc Natl Acad Sci U S A       Date:  1983-01       Impact factor: 11.205

5.  Effects of bacteriophage f1 gene III protein on the host cell membrane.

Authors:  J D Boeke; P Model; N D Zinder
Journal:  Mol Gen Genet       Date:  1982

6.  Membrane biogenesis: cotranslational integration of the bacteriophage f1 coat protein into an Escherichia coli membrane fraction.

Authors:  C N Chang; P Model; G Blobel
Journal:  Proc Natl Acad Sci U S A       Date:  1979-03       Impact factor: 11.205

7.  Gene-III protein of filamentous phages: evidence for a carboxyl-terminal domain with a role in morphogenesis.

Authors:  J W Crissman; G P Smith
Journal:  Virology       Date:  1984-01-30       Impact factor: 3.616

8.  Studies on transformation of Escherichia coli with plasmids.

Authors:  D Hanahan
Journal:  J Mol Biol       Date:  1983-06-05       Impact factor: 5.469

9.  Generation of diverse high-affinity human monoclonal antibodies by repertoire cloning.

Authors:  M A Persson; R H Caothien; D R Burton
Journal:  Proc Natl Acad Sci U S A       Date:  1991-03-15       Impact factor: 11.205

Review 10.  Human and mouse monoclonal antibodies by repertoire cloning.

Authors:  D R Burton
Journal:  Trends Biotechnol       Date:  1991-05       Impact factor: 19.536
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  253 in total

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6.  Phage antibodies neutralize vaccinia virus.

Authors:  N V Tikunova; E F Belanov; V V Morozova; T A Batanova; N I Bormotov; L G Ovechkina; A A Ilyichev; L S Sandakhchiev
Journal:  Dokl Biochem Biophys       Date:  2002 Jan-Feb       Impact factor: 0.788

7.  Making artificial antibodies: a format for phage display of combinatorial heterodimeric arrays.

Authors:  C Gao; S Mao; C H Lo; P Wirsching; R A Lerner; K D Janda
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Authors:  H J Ditzel; U Garrigues; C B Andersen; M K Larsen; H J Garrigues; A Svejgaard; I Hellström; K E Hellström; J C Jensenius
Journal:  Proc Natl Acad Sci U S A       Date:  1997-07-22       Impact factor: 11.205

Review 9.  Yeast killer systems.

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10.  Anti-secretogranin III therapy of oxygen-induced retinopathy with optimal safety.

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