Literature DB >> 34057717

Immunophenotyping of the Medullary B Cell Compartment In Mouse Models.

Amélie Bonaud1, Karl Balabanian2, Marion Espéli3.   

Abstract

B cell development is a stepwise process encompassing several B cell precursor stages that can be phenotypically distinguished, and that is taking place in the bone marrow in adults. Interestingly, within the bone marrow B cell precursors coexist with the most differentiated actors of this lineage, the plasma cells. In this chapter, we describe a method to recover cells from the bone marrow and a flow cytometric approach to identify each subpopulation of the B cell lineage that resides within the bone marrow compartment. This protocol focuses on membrane markers to discriminate all the B cell subpopulations in order to preserve cell integrity during experimentation and for further analyses.

Entities:  

Keywords:  B cell lineage; Bone marrow; Flow cytometry; Immature B cell; Mature B cell; Plasma cell; Pre-B cell; Prepro-B cell; Pro-B cell

Year:  2021        PMID： 34057717     DOI： 10.1007/978-1-0716-1425-9_8

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  23 in total

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Authors:  Lynn M Corcoran; David M Tarlinton
Journal:  Curr Opin Immunol       Date:  2016-01-20       Impact factor: 7.486

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Journal:  Immunity       Date:  2007-06       Impact factor: 31.745

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Authors:  Ismail Dogan; Barbara Bertocci; Valérie Vilmont; Frédéric Delbos; Jérome Mégret; Sébastien Storck; Claude-Agnès Reynaud; Jean-Claude Weill
Journal:  Nat Immunol       Date:  2009-10-25       Impact factor: 25.606

4.  Early production of intracellular IgM by B-lymphocyte precursors in mouse.

Authors:  M C Raff; M Megson; J J Owen; M D Cooper
Journal:  Nature       Date:  1976-01-22       Impact factor: 49.962

5.  A new staining protocol for detection of murine antibody-secreting plasma cell subsets by flow cytometry.

Authors:  Katharina Pracht; Julia Meinzinger; Patrick Daum; Sebastian R Schulz; Dorothea Reimer; Manuela Hauke; Edith Roth; Dirk Mielenz; Claudia Berek; Joana Côrte-Real; Hans-Martin Jäck; Wolfgang Schuh
Journal:  Eur J Immunol       Date:  2017-07-13       Impact factor: 5.532

6.  Reporter gene insertions reveal a strictly B lymphoid-specific expression pattern of Pax5 in support of its B cell identity function.

Authors:  Martin Fuxa; Meinrad Busslinger
Journal:  J Immunol       Date:  2007-06-15       Impact factor: 5.422

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Authors:  R L Coffman; I L Weissman
Journal:  J Exp Med       Date:  1981-02-01       Impact factor: 14.307

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Journal:  J Exp Med       Date:  1987-02-01       Impact factor: 14.307

Review 9.  The Role of the Pre-B Cell Receptor in B Cell Development, Repertoire Selection, and Tolerance.

Authors:  Thomas H Winkler; Inga-Lill Mårtensson
Journal:  Front Immunol       Date:  2018-11-15       Impact factor: 7.561

Review 10.  In the Right Place, at the Right Time: Spatiotemporal Conditions Determining Plasma Cell Survival and Function.

Authors:  Randall L Lindquist; Raluca A Niesner; Anja E Hauser
Journal:  Front Immunol       Date:  2019-04-24       Impact factor: 7.561
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