Literature DB >> 28608550

A new staining protocol for detection of murine antibody-secreting plasma cell subsets by flow cytometry.

Katharina Pracht1, Julia Meinzinger1, Patrick Daum1, Sebastian R Schulz1, Dorothea Reimer1, Manuela Hauke1, Edith Roth1, Dirk Mielenz1, Claudia Berek2, Joana Côrte-Real1, Hans-Martin Jäck1, Wolfgang Schuh1.   

Abstract

We provide a robust four-color fluorescence-based flow cytometry protocol that distinguishes viable dividing plasmablasts from nondividing plasma cells and, based on CD19 surface abundance, identifies two mature plasma cell populations in the spleen and the bone marrow of mice.
© 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Entities:  

Keywords:  B220; Blimp1; CD138; CD19; Flow cytometry; Long-lived plasma cell; Plasmablast; TACI

Mesh：

Substances：

Year:  2017        PMID： 28608550     DOI： 10.1002/eji.201747019

Source DB:  PubMed          Journal:  Eur J Immunol        ISSN： 0014-2980            Impact factor:   5.532


  44 in total

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9.  IgM, IgG, and IgA Influenza-Specific Plasma Cells Express Divergent Transcriptomes.

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Journal:  J Immunol       Date:  2019-09-09       Impact factor: 5.422

10.  TFG is required for autophagy flux and to prevent endoplasmic reticulum stress in CH12 B lymphoma cells.

Authors:  Tobit D Steinmetz; Ursula Schlötzer-Schrehardt; Abigail Hearne; Wolfgang Schuh; Jens Wittner; Sebastian R Schulz; Thomas H Winkler; Hans-Martin Jäck; Dirk Mielenz
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