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Literature DB >> 3402436

Vaccinia virus late transcripts generated in vitro have a poly(A) head.

Abstract

A cell free system mediating accurate transcription of vaccinia virus genes was established using lysates of cells in the late phase of infection. Vaccinia late genes are faithfully transcribed in this extract whereas cellular pol II and pol III promoters are not recognized. The late viral transcripts contain a poly(A) head of approximately 35 nt at the 5' end which is not co-linearly encoded in the externally added template. The transcripts obtained in vitro are indistinguishable from the mature in vivo RNAs. The poly(A) head is synthesized de novo and its formation appears to be directly coupled to the transcription of the gene. The synthesis of the poly(A) head transcripts in vitro is consistent with a proposed slippage model.

Entities: Chemical Disease Species

Mesh：

Substances：
Poly A

Year: 1988 PMID： 3402436 PMCID： PMC454454

Source DB: PubMed Journal: EMBO J ISSN： 0261-4189 Impact factor: 11.598

31 in total

1. Major transcript of the frameshifted coxII gene from trypanosome mitochondria contains four nucleotides that are not encoded in the DNA.

Authors: R Benne; J Van den Burg; J P Brakenhoff; P Sloof; J H Van Boom; M C Tromp
Journal: Cell Date: 1986-09-12 Impact factor: 41.582

2. 5'-Terminal structure and mRNA stability.

Authors: Y Furuichi; A LaFiandra; A J Shatkin
Journal: Nature Date: 1977-03-17 Impact factor: 49.962

3. Simple, efficient in vitro synthesis of capped RNA useful for direct expression of cloned eukaryotic genes.

Authors: R Contreras; H Cheroutre; W Degrave; W Fiers
Journal: Nucleic Acids Res Date: 1982-10-25 Impact factor: 16.971

4. Sequence relationships between the genome and the intracellular RNA species 1, 3, 6, and 7 of mouse hepatitis virus strain A59.

Authors: W J Spaan; P J Rottier; M C Horzinek; B A van der Zeijst
Journal: J Virol Date: 1982-05 Impact factor: 5.103

5. Purification and analysis of a factor which suppresses nick-induced transcription by RNA polymerase II and its identity with poly(ADP-ribose) polymerase.

Authors: E Slattery; J D Dignam; T Matsui; R G Roeder
Journal: J Biol Chem Date: 1983-05-10 Impact factor: 5.157

6. DNA-dependent transcription of adenovirus genes in a soluble whole-cell extract.

Authors: J L Manley; A Fire; A Cano; P A Sharp; M L Gefter
Journal: Proc Natl Acad Sci U S A Date: 1980-07 Impact factor: 11.205

7. A control region in the center of the 5S RNA gene directs specific initiation of transcription: I. The 5' border of the region.

Authors: S Sakonju; D F Bogenhagen; D D Brown
Journal: Cell Date: 1980-01 Impact factor: 41.582

8. Selected host cell capped RNA fragments prime influenza viral RNA transcription in vivo.

Authors: A R Beaton; R M Krug
Journal: Nucleic Acids Res Date: 1981-09-11 Impact factor: 16.971

9. Distinctive nucleotide sequences adjacent to multiple initiation and termination sites of an early vaccinia virus gene.

Authors: S Venkatesan; B M Baroudy; B Moss
Journal: Cell Date: 1981-09 Impact factor: 41.582

7. Identification of the vaccinia virus gene encoding an 18-kilodalton subunit of RNA polymerase and demonstration of a 5' poly(A) leader on its early transcript.

Authors: B Y Ahn; E V Jones; B Moss
Journal: J Virol Date: 1990-06 Impact factor: 5.103

Vaccinia virus late transcripts generated in vitro have a poly(A) head.

1. Major transcript of the frameshifted coxII gene from trypanosome mitochondria contains four nucleotides that are not encoded in the DNA.

2. 5'-Terminal structure and mRNA stability.

3. Simple, efficient in vitro synthesis of capped RNA useful for direct expression of cloned eukaryotic genes.

4. Sequence relationships between the genome and the intracellular RNA species 1, 3, 6, and 7 of mouse hepatitis virus strain A59.

5. Purification and analysis of a factor which suppresses nick-induced transcription by RNA polymerase II and its identity with poly(ADP-ribose) polymerase.

6. DNA-dependent transcription of adenovirus genes in a soluble whole-cell extract.

7. A control region in the center of the 5S RNA gene directs specific initiation of transcription: I. The 5' border of the region.

8. Selected host cell capped RNA fragments prime influenza viral RNA transcription in vivo.

9. Distinctive nucleotide sequences adjacent to multiple initiation and termination sites of an early vaccinia virus gene.

10. Selective transcription of vaccinia virus genes in template dependent soluble extracts of infected cells.

1. A transcription factor for expression of vaccinia virus late genes is encoded by an intermediate gene.

2. Editing of the Sendai virus P/C mRNA by G insertion occurs during mRNA synthesis via a virus-encoded activity.

3. Anti-mRNAs in La Crosse bunyavirus-infected cells.

4. Poly(A) leader of eukaryotic mRNA bypasses the dependence of translation on initiation factors.

5. Transcription elongation complex stability: the topological lock.

Review 6. Messenger RNA editing and the genetic code.

7. Identification of the vaccinia virus gene encoding an 18-kilodalton subunit of RNA polymerase and demonstration of a 5' poly(A) leader on its early transcript.

8. Identification of factors specific for transcription of the late class of vaccinia virus genes.

9. Capped poly(A) leaders of variable lengths at the 5' ends of vaccinia virus late mRNAs.

10. Vaccinia virus gene A18R encodes an essential DNA helicase.