Literature DB >> 33963

Purification and properties of the manganese-dependent phosphoglycerate mutase of Bacillus subtilis.

K Watabe, E Freese.   

Abstract

Phosphoglycerate mutase of Bacillus subtilis was purified to apparent homogeneity. It specifically required manganese ions for stability and activity, but it does not need 2,3-diphosphoglycerate as cofactor; the Km for Mn2+ is about 4.5 micrometer. Enzyme activity was inhibited by heavy-metal ions, 2,3-butanedione, and sulfhydryl agents. The mutase has a molecular weight of about 74,000 as shown by Sephadex gel filtration and by acrylamide gel electrophoresis in the presence of sodium dodecyl sulfate; it consisted of one polypeptide.

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Year:  1979        PMID： 33963      PMCID： PMC218356          DOI： 10.1128/jb.137.2.773-778.1979

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  19 in total

1.  DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.

Authors:  B J DAVIS
Journal:  Ann N Y Acad Sci       Date:  1964-12-28       Impact factor: 5.691

2.  Properties of the phosphoprotein-phosphoglyceric mutase.

Authors:  L I PIZER
Journal:  J Biol Chem       Date:  1960-04       Impact factor: 5.157

3.  Purification and some properties of phosphorylglyceric acid mutase from rabbit skeletal muscle.

Authors:  R W COWGILL; L I PIZER
Journal:  J Biol Chem       Date:  1956-12       Impact factor: 5.157

4.  Phosphoglycerate mutase has essential arginyl residues.

Authors:  C L Borders; B A Wilson
Journal:  Biochem Biophys Res Commun       Date:  1976-12-20       Impact factor: 3.575

5.  Phosphoglycerate mutase from yeast, chicken breast muscle, and kidney (2, 3-PGA-dependent).

Authors:  S Grisolia; J Carreras
Journal:  Methods Enzymol       Date:  1975       Impact factor: 1.600

6.  Studies on phosphoglyceromutase from chicken breast muscle: number and reactivity of sulfhydryl groups.

Authors:  T J Carne; D J McKay; T G Flynn
Journal:  Can J Biochem       Date:  1976-04

7.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

8.  Levels of small molecules and enzymes in the mother cell compartment and the forespore of sporulating Bacillus megaterium.

Authors:  R P Singh; B Setlow; P Setlow
Journal:  J Bacteriol       Date:  1977-06       Impact factor: 3.490

9.  Detection of an essential sulfhydryl group in phosphoglycerate mutase with an affinity-labeling reagent.

Authors:  F C Hartman; I L Norton
Journal:  J Biol Chem       Date:  1976-08-10       Impact factor: 5.157

10.  Manganese requirement of phosphoglycerate phosphomutase and its consequences for growth and sporulation of Bacillus subtilis.

Authors:  Y K Oh; E Freese
Journal:  J Bacteriol       Date:  1976-08       Impact factor: 3.490
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  12 in total

1.  A superfamily of metalloenzymes unifies phosphopentomutase and cofactor-independent phosphoglycerate mutase with alkaline phosphatases and sulfatases.

Authors:  M Y Galperin; A Bairoch; E V Koonin
Journal:  Protein Sci       Date:  1998-08       Impact factor: 6.725

2.  Purification and properties of phosphoglycerate phosphomutase from spores and cells of Bacillus megaterium.

Authors:  R P Singh; P Setlow
Journal:  J Bacteriol       Date:  1979-02       Impact factor: 3.490

3.  Structure and mechanism of action of a novel phosphoglycerate mutase from Bacillus stearothermophilus.

Authors:  M J Jedrzejas; M Chander; P Setlow; G Krishnasamy
Journal:  EMBO J       Date:  2000-04-03       Impact factor: 11.598

4.  Treponema pallidum 3-phosphoglycerate mutase is a heat-labile enzyme that may limit the maximum growth temperature for the spirochete.

Authors:  S Benoit; J E Posey; M R Chenoweth; F C Gherardini
Journal:  J Bacteriol       Date:  2001-08       Impact factor: 3.490

5.  Do metal ions promote the re-activation of the 2,3-bisphosphoglycerate-independent phosphoglycerate mutases?

Authors:  C M Johnson; N C Price
Journal:  Biochem J       Date:  1988-05-15       Impact factor: 3.857

6.  Regulation of phosphoglycerate phosphomutase in developing forespores and dormant and germinated spores of Bacillus megaterium by the level of free manganous ions.

Authors:  R P Singh; P Setlow
Journal:  J Bacteriol       Date:  1979-09       Impact factor: 3.490

7.  Phosphoprotein with phosphoglycerate mutase activity from the archaeon Sulfolobus solfataricus.

Authors:  M Ben Potters; Barbara T Solow; Kenneth M Bischoff; David E Graham; Brian H Lower; Richard Helm; Peter J Kennelly
Journal:  J Bacteriol       Date:  2003-04       Impact factor: 3.490

8.  The internal pH of the forespore compartment of Bacillus megaterium decreases by about 1 pH unit during sporulation.

Authors:  N G Magill; A E Cowan; D E Koppel; P Setlow
Journal:  J Bacteriol       Date:  1994-04       Impact factor: 3.490

9.  Cloning and nucleotide sequences of the genes encoding triose phosphate isomerase, phosphoglycerate mutase, and enolase from Bacillus subtilis.

Authors:  M A Leyva-Vazquez; P Setlow
Journal:  J Bacteriol       Date:  1994-07       Impact factor: 3.490

10.  Analysis of the relationship between the decrease in pH and accumulation of 3-phosphoglyceric acid in developing forespores of Bacillus species.

Authors:  N G Magill; A E Cowan; M A Leyva-Vazquez; M Brown; D E Koppel; P Setlow
Journal:  J Bacteriol       Date:  1996-04       Impact factor: 3.490
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