Literature DB >> 33959

Purification and properties of phosphoglycerate phosphomutase from spores and cells of Bacillus megaterium.

R P Singh, P Setlow.   

Abstract

Phosphoglycerate phosphomutase has been purified to homogeneity from vegetative cells and germinated spores of Bacillus megaterium, and the spore and cell enzymes appear identical. The enzyme is a monomer of molecular weight 61,000. The compound 2,3-diphosphoglyceric acid is not required for activity, but the enzyme has an absolute and specific requirement for Mn2+. The enzyme is inhibited by ethylenediaminetetraacetate and sulfhydryl reagents, has a pH optimum of about 8.0, and has Km values for 3-phosphoglyceric acid and Mn2+ of 5 x 10(-4) and 4 x 10(-5) M, respectively.

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Year:  1979        PMID： 33959      PMCID： PMC218391          DOI： 10.1128/jb.137.2.1024-1027.1979

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  10 in total

1.  Phosphoglycerate mutase from yeast, chicken breast muscle, and kidney (2, 3-PGA-dependent).

Authors:  S Grisolia; J Carreras
Journal:  Methods Enzymol       Date:  1975       Impact factor: 1.600

2.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

3.  Phosphoglycerate mutase in developing forespores of Bacillus megaterium may be regulated by the intrasporal level of free manganous ion.

Authors:  R P Singh; P Setlow
Journal:  Biochem Biophys Res Commun       Date:  1978-05-15       Impact factor: 3.575

4.  Purification and properties of the manganese-dependent phosphoglycerate mutase of Bacillus subtilis.

Authors:  K Watabe; E Freese
Journal:  J Bacteriol       Date:  1979-02       Impact factor: 3.490

5.  Enolase from spores and cells of Bacillus megaterium: two-step purification of the enzyme and some of its properties.

Authors:  R P Singh; P Setlow
Journal:  J Bacteriol       Date:  1978-04       Impact factor: 3.490

6.  Biochemical studies of bacterial sporulation and germination. XXII. Energy metabolism in early stages of germination of Bacillus megaterium spores.

Authors:  P Setlow; A Kornberg
Journal:  J Biol Chem       Date:  1970-07-25       Impact factor: 5.157

7.  The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.

Authors:  K Weber; M Osborn
Journal:  J Biol Chem       Date:  1969-08-25       Impact factor: 5.157

8.  Purification and properties of a specific proteolytic enzyme present in spores of Bacillus magaterium.

Authors:  P Setlow
Journal:  J Biol Chem       Date:  1976-12-25       Impact factor: 5.157

9.  Levels of small molecules and enzymes in the mother cell compartment and the forespore of sporulating Bacillus megaterium.

Authors:  R P Singh; B Setlow; P Setlow
Journal:  J Bacteriol       Date:  1977-06       Impact factor: 3.490

10.  Manganese requirement of phosphoglycerate phosphomutase and its consequences for growth and sporulation of Bacillus subtilis.

Authors:  Y K Oh; E Freese
Journal:  J Bacteriol       Date:  1976-08       Impact factor: 3.490
  10 in total
  14 in total

1.  A superfamily of metalloenzymes unifies phosphopentomutase and cofactor-independent phosphoglycerate mutase with alkaline phosphatases and sulfatases.

Authors:  M Y Galperin; A Bairoch; E V Koonin
Journal:  Protein Sci       Date:  1998-08       Impact factor: 6.725

2.  Purification and properties of the manganese-dependent phosphoglycerate mutase of Bacillus subtilis.

Authors:  K Watabe; E Freese
Journal:  J Bacteriol       Date:  1979-02       Impact factor: 3.490

3.  Structure and mechanism of action of a novel phosphoglycerate mutase from Bacillus stearothermophilus.

Authors:  M J Jedrzejas; M Chander; P Setlow; G Krishnasamy
Journal:  EMBO J       Date:  2000-04-03       Impact factor: 11.598

4.  Effects of Mn and Fe levels on Bacillus subtilis spore resistance and effects of Mn2+, other divalent cations, orthophosphate, and dipicolinic acid on protein resistance to ionizing radiation.

Authors:  Amanda C Granger; Elena K Gaidamakova; Vera Y Matrosova; Michael J Daly; Peter Setlow
Journal:  Appl Environ Microbiol       Date:  2010-11-05       Impact factor: 4.792

5.  Treponema pallidum 3-phosphoglycerate mutase is a heat-labile enzyme that may limit the maximum growth temperature for the spirochete.

Authors:  S Benoit; J E Posey; M R Chenoweth; F C Gherardini
Journal:  J Bacteriol       Date:  2001-08       Impact factor: 3.490

6.  Measurements of the pH within dormant and germinated bacterial spores.

Authors:  B Setlow; P Setlow
Journal:  Proc Natl Acad Sci U S A       Date:  1980-05       Impact factor: 11.205

7.  Do metal ions promote the re-activation of the 2,3-bisphosphoglycerate-independent phosphoglycerate mutases?

Authors:  C M Johnson; N C Price
Journal:  Biochem J       Date:  1988-05-15       Impact factor: 3.857

8.  Phosphoprotein with phosphoglycerate mutase activity from the archaeon Sulfolobus solfataricus.

Authors:  M Ben Potters; Barbara T Solow; Kenneth M Bischoff; David E Graham; Brian H Lower; Richard Helm; Peter J Kennelly
Journal:  J Bacteriol       Date:  2003-04       Impact factor: 3.490

9.  The internal pH of the forespore compartment of Bacillus megaterium decreases by about 1 pH unit during sporulation.

Authors:  N G Magill; A E Cowan; D E Koppel; P Setlow
Journal:  J Bacteriol       Date:  1994-04       Impact factor: 3.490

10.  Cloning and nucleotide sequences of the genes encoding triose phosphate isomerase, phosphoglycerate mutase, and enolase from Bacillus subtilis.

Authors:  M A Leyva-Vazquez; P Setlow
Journal:  J Bacteriol       Date:  1994-07       Impact factor: 3.490
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