BACKGROUND: There is much data available concerning the initiation of the immune response after SARS-CoV-2 infection, but long-term data are scarce. METHODS: We thus longitudinally evaluated and compared the total and neutralizing immune response of 61 patients to SARS-CoV-2 infection up to eight months after diagnosis by RT-PCR using several commercial assays. RESULTS: Among the 208 samples tested, the percentage of seropositivity was comparable between assays up to four months after diagnosis and then tended to be more heterogeneous between assays (p < 0.05). The percentage of patients with a neutralizing titer decreased from 82% before two months postdiagnosis to 57% after six months. This decrease appeared to be more marked for patients under 65 years old and those not requiring hospitalization. The percentage of serology reversion at 6 months was from 11% with the WANTAI total assay to over 39% with the ABBOTT IgG assay. The neutralizing antibody titers decreased in parallel with the decrease of total antibody titers, with important heterogeneity between assays. CONCLUSIONS: In conclusion, serological tests show equivalent sensitivity in the first months after the diagnosis of SARS-CoV-2 infection, but their performance later, postinfection, must be considered when interpreting the results.
BACKGROUND: There is much data available concerning the initiation of the immune response after SARS-CoV-2 infection, but long-term data are scarce. METHODS: We thus longitudinally evaluated and compared the total and neutralizing immune response of 61 patients to SARS-CoV-2 infection up to eight months after diagnosis by RT-PCR using several commercial assays. RESULTS: Among the 208 samples tested, the percentage of seropositivity was comparable between assays up to four months after diagnosis and then tended to be more heterogeneous between assays (p < 0.05). The percentage of patients with a neutralizing titer decreased from 82% before two months postdiagnosis to 57% after six months. This decrease appeared to be more marked for patients under 65 years old and those not requiring hospitalization. The percentage of serology reversion at 6 months was from 11% with the WANTAI total assay to over 39% with the ABBOTT IgG assay. The neutralizing antibody titers decreased in parallel with the decrease of total antibody titers, with important heterogeneity between assays. CONCLUSIONS: In conclusion, serological tests show equivalent sensitivity in the first months after the diagnosis of SARS-CoV-2 infection, but their performance later, postinfection, must be considered when interpreting the results.
Authors: Lewis F Buss; Carlos A Prete; Claudia M M Abrahim; Alfredo Mendrone; Tassila Salomon; Cesar de Almeida-Neto; Rafael F O França; Maria C Belotti; Maria P S S Carvalho; Allyson G Costa; Myuki A E Crispim; Suzete C Ferreira; Nelson A Fraiji; Susie Gurzenda; Charles Whittaker; Leonardo T Kamaura; Pedro L Takecian; Pedro da Silva Peixoto; Marcio K Oikawa; Anna S Nishiya; Vanderson Rocha; Nanci A Salles; Andreza Aruska de Souza Santos; Martirene A da Silva; Brian Custer; Kris V Parag; Manoel Barral-Netto; Moritz U G Kraemer; Rafael H M Pereira; Oliver G Pybus; Michael P Busch; Márcia C Castro; Christopher Dye; Vítor H Nascimento; Nuno R Faria; Ester C Sabino Journal: Science Date: 2020-12-08 Impact factor: 47.728
Authors: Alison Tarke; John Sidney; Conner K Kidd; Jennifer M Dan; Sydney I Ramirez; Esther Dawen Yu; Jose Mateus; Ricardo da Silva Antunes; Erin Moore; Paul Rubiro; Nils Methot; Elizabeth Phillips; Simon Mallal; April Frazier; Stephen A Rawlings; Jason A Greenbaum; Bjoern Peters; Davey M Smith; Shane Crotty; Daniela Weiskopf; Alba Grifoni; Alessandro Sette Journal: Cell Rep Med Date: 2021-01-26
Authors: Kevin R McCarthy; Linda J Rennick; Sham Nambulli; Lindsey R Robinson-McCarthy; William G Bain; Ghady Haidar; W Paul Duprex Journal: Science Date: 2021-02-03 Impact factor: 47.728
Authors: Jennifer M Dan; Jose Mateus; Yu Kato; Kathryn M Hastie; Esther Dawen Yu; Caterina E Faliti; Alba Grifoni; Sydney I Ramirez; Sonya Haupt; April Frazier; Catherine Nakao; Vamseedhar Rayaprolu; Stephen A Rawlings; Bjoern Peters; Florian Krammer; Viviana Simon; Erica Ollmann Saphire; Davey M Smith; Daniela Weiskopf; Alessandro Sette; Shane Crotty Journal: Science Date: 2021-01-06 Impact factor: 47.728
Authors: Ana B Gómez-Belda; Mar Fernández-Garcés; Elisabeth Mateo-Sanchis; Manuel Madrazo; Mar Carmona; Laura Piles-Roger; Arturo Artero Journal: Geriatr Gerontol Int Date: 2020-12-02 Impact factor: 2.730