Aim: Neonatal necrotizing enterocolitis (NEC) is a deadly and unpredictable gastrointestinal disease, for which no biomarker exists. We aimed to describe the methylation patterns in stool and colon from infants with NEC. Methods: We performed a high-resolution genome-wide epigenomic analysis using solution-phase hybridization and next-generation sequencing of bisulfite-converted DNA. Results: Our data reveal significant genomic hypermethylation in NEC tissues compared with non-NEC controls. These changes were more pronounced in regions outside CpG islands and gene regulatory elements, suggesting that NEC-specific hypermethylation is not a nonspecific global phenomenon. Conclusions: This study provides evidence of a methylomic signature associated with NEC that is detectable noninvasively and provides a new opportunity for the development of a novel diagnostic method for NEC.
Aim: Neonatal necrotizing enterocolitis (NEC) is a deadly and unpredictable gastrointestinal disease, for which no biomarker exists. We aimed to describe the methylation patterns in stool and colon from infants with NEC. Methods: We performed a high-resolution genome-wide epigenomic analysis using solution-phase hybridization and next-generation sequencing of bisulfite-converted DNA. Results: Our data reveal significant genomic hypermethylation in NEC tissues compared with non-NEC controls. These changes were more pronounced in regions outside CpG islands and gene regulatory elements, suggesting that NEC-specific hypermethylation is not a nonspecific global phenomenon. Conclusions: This study provides evidence of a methylomic signature associated with NEC that is detectable noninvasively and provides a new opportunity for the development of a novel diagnostic method for NEC.
Authors: Anne Jan van der Meer; Anna Kroeze; Arie J Hoogendijk; Aicha Ait Soussan; C Ellen van der Schoot; Walter A Wuillemin; Carlijn Voermans; Tom van der Poll; Sacha Zeerleder Journal: Blood Adv Date: 2019-03-12
Authors: Zhenwu Lin; John P Hegarty; Wei Yu; Jon A Cappel; Xi Chen; Pieter W Faber; Yunhua Wang; Lisa S Poritz; Jian-Bing Fan; Walter A Koltun Journal: Dig Dis Sci Date: 2012-07-21 Impact factor: 3.199
Authors: Ravi M Patel; Sarah Kandefer; Michele C Walsh; Edward F Bell; Waldemar A Carlo; Abbot R Laptook; Pablo J Sánchez; Seetha Shankaran; Krisa P Van Meurs; M Bethany Ball; Ellen C Hale; Nancy S Newman; Abhik Das; Rosemary D Higgins; Barbara J Stoll Journal: N Engl J Med Date: 2015-01-22 Impact factor: 91.245
Authors: Timothy A Blauwkamp; Simone Thair; Judith C Wilber; Samuel Yang; Michael J Rosen; Lily Blair; Martin S Lindner; Igor D Vilfan; Trupti Kawli; Fred C Christians; Shivkumar Venkatasubrahmanyam; Gregory D Wall; Anita Cheung; Zoë N Rogers; Galit Meshulam-Simon; Liza Huijse; Sanjeev Balakrishnan; James V Quinn; Desiree Hollemon; David K Hong; Marla Lay Vaughn; Mickey Kertesz; Sivan Bercovici Journal: Nat Microbiol Date: 2019-02-11 Impact factor: 17.745
Authors: Elaine R Nimmo; James G Prendergast; Marian C Aldhous; Nicholas A Kennedy; Paul Henderson; Hazel E Drummond; Bernard H Ramsahoye; David C Wilson; Colin A Semple; Jack Satsangi Journal: Inflamm Bowel Dis Date: 2011-10-21 Impact factor: 5.325
Authors: Thomas Karlas; Lara Weise; Stephanie Kuhn; Felix Krenzien; Matthias Mehdorn; David Petroff; Nicolas Linder; Alexander Schaudinn; Harald Busse; Volker Keim; Johann Pratschke; Johannes Wiegand; Katrin Splith; Moritz Schmelzle Journal: J Transl Med Date: 2017-05-19 Impact factor: 5.531
Authors: Tianjiao Chu; Patricia A Shaw; Suveyda Yeniterzi; Mary Dunkel; Aleksander Rajkovic; W Allen Hogge; Kimberly D Bunce; David G Peters Journal: PLoS One Date: 2017-03-17 Impact factor: 3.240
Authors: L J W Bosch; V Melotte; S Mongera; K L J Daenen; V M H Coupé; S T van Turenhout; E M Stoop; T R de Wijkerslooth; C J J Mulder; C Rausch; E J Kuipers; E Dekker; M J Domanico; G P Lidgard; B M Berger; M van Engeland; B Carvalho; G A Meijer Journal: Am J Gastroenterol Date: 2019-12 Impact factor: 10.864