| Literature DB >> 33883548 |
Matthew H Larson1, Wenying Pan2, Hyunsung John Kim2, Ruth E Mauntz2, Sarah M Stuart2, Monica Pimentel2, Yiqi Zhou2, Per Knudsgaard2, Vasiliki Demas2, Alexander M Aravanis2, Arash Jamshidi2.
Abstract
Cell-free RNA (cfRNA) is a promising analyte for cancer detection. However, a comprehensive assessment of cfRNA in individuals with and without cancer has not been conducted. We perform the first transcriptome-wide characterization of cfRNA in cancer (stage III breast [n = 46], lung [n = 30]) and non-cancer (n = 89) participants from the Circulating Cell-free Genome Atlas (NCT02889978). Of 57,820 annotated genes, 39,564 (68%) are not detected in cfRNA from non-cancer individuals. Within these low-noise regions, we identify tissue- and cancer-specific genes, defined as "dark channel biomarker" (DCB) genes, that are recurrently detected in individuals with cancer. DCB levels in plasma correlate with tumor shedding rate and RNA expression in matched tissue, suggesting that DCBs with high expression in tumor tissue could enhance cancer detection in patients with low levels of circulating tumor DNA. Overall, cfRNA provides a unique opportunity to detect cancer, predict the tumor tissue of origin, and determine the cancer subtype.Entities:
Mesh：
Substances：
Year: 2021 PMID： 33883548 PMCID： PMC8060291 DOI： 10.1038/s41467-021-22444-1
Source DB: PubMed Journal: Nat Commun ISSN： 2041-1723 Impact factor: 17.694