Literature DB >> 319715

Development and evaluation of an enzyme-labeled antibody test for the rapid detection of hog cholera antibodies.

G C Saunders.   

Abstract

A rapid enzyme-labeled antibody (ELA) microtechnique for the screening of swine for hog cholera antibodies was developed and evaluated with a blind study, using a 640-sample hog cholera serum bank. The total time to run a group of 22 samples was approximately 1 hour. The ELA test results correlated greater than 99% with hog cholera serum-neutralization test results on the same serums. Test results also indicated that the ELA test shares with the hog cholera serum-neutralization test the problem of cross reactions between the antibodies of hog cholera and bovine viral diarrhea.

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Year:  1977        PMID: 319715

Source DB:  PubMed          Journal:  Am J Vet Res        ISSN: 0002-9645            Impact factor:   1.156


  12 in total

1.  Comparison of serological methods for the detection of B. abortus antibodies in sera from vaccinated and non-vaccinated cattle.

Authors:  F C Heck; J D Williams; R P Crawford; A I Flowers
Journal:  J Hyg (Lond)       Date:  1979-12

2.  Double-antibody solid-phase enzyme immunoassay for the detection of staphylococcal enterotoxin A.

Authors:  G C Saunders; M L Bartlett
Journal:  Appl Environ Microbiol       Date:  1977-11       Impact factor: 4.792

3.  Detection of antibodies against hog cholera virus and bovine viral diarrhea virus in porcine serum. A comparative examination using CF, PLA and NPLA assays.

Authors:  M H Jensen
Journal:  Acta Vet Scand       Date:  1981       Impact factor: 1.695

4.  Interpretation of spectrophotometric absorbance values to define results of enzyme-linked immunosorbent assays.

Authors:  F C Heck; J D Williams; J Pruett
Journal:  J Clin Microbiol       Date:  1980-04       Impact factor: 5.948

Review 5.  Enzymes as versatile labels and signal amplifiers for monitoring immunochemical reactions.

Authors:  T T Ngo; H M Lenhoff
Journal:  Mol Cell Biochem       Date:  1982-04-16       Impact factor: 3.396

6.  Antigen requirements and specificity of a microplate enzyme-linked immunosorbent assay (ELISA) for detecting infectious bronchitis viral antibodies in chicken serum.

Authors:  A Soula; Y Moreau
Journal:  Arch Virol       Date:  1981       Impact factor: 2.574

7.  Cross-reactivity of Haemophilus somnus antibody in agglutination and complement fixation tests and in the enzyme-linked immunosorbent assay.

Authors:  J Canto; E L Biberstein; T A Schulte; D Behymer
Journal:  J Clin Microbiol       Date:  1983-03       Impact factor: 5.948

8.  Development of an enzyme-linked immunosorbent assay for detecting antibodies in sera of Brucella suis-infected swine.

Authors:  C O Thoen; M P Hopkins; A L Armbrust; R D Angus; D E Pietz
Journal:  Can J Comp Med       Date:  1980-07

9.  Enzyme-linked immunosorbent assay for detecting antibodies in cattle in a herd in which anaplasmosis was diagnosed.

Authors:  C O Thoen; B Blackburn; K Mills; J Lomme; M P Hopkins
Journal:  J Clin Microbiol       Date:  1980-05       Impact factor: 5.948

10.  Enzyme-linked immunosorbent assay compared with immunoprecipitation for serotyping Neisseria meningitidis and its use in demonstrating serotype polymorphism.

Authors:  E B Mackie; L A White; K N Brown; L E Bryan
Journal:  J Clin Microbiol       Date:  1981-03       Impact factor: 5.948

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