Matthew J Murray1, Megan McIntosh2, Claire Atkinson2, Tabitha Mahungu3, Edward Wright4, Wendy Chatterton5, Michael Gandy5, Matthew B Reeves6. 1. Institute of Immunity & Transplantation, University College London, Royal Free Campus, London NW3 2PF, UK. Electronic address: matthew.murray@ucl.ac.uk. 2. Institute of Immunity & Transplantation, University College London, Royal Free Campus, London NW3 2PF, UK. 3. Department of Infectious Diseases, Royal Free Hospital London NHS Foundation Trust, London NW3 2PF, UK. 4. School of Life Sciences, University of Sussex, Brighton, UK. 5. Health Services Laboratories LLP, London, UK. 6. Institute of Immunity & Transplantation, University College London, Royal Free Campus, London NW3 2PF, UK. Electronic address: matthew.reeves@ucl.ac.uk.
Abstract
OBJECTIVES: To assess whether a commercially available CE-IVD, ELISA-based surrogate neutralisation assay (cPass, Genscript) provides a genuine measure of SARS-CoV-2 neutralisation by human sera, and further to establish whether measuring responses against the RBD of S was a diagnostically useful proxy for responses against the whole S protein. METHODS: Serum samples from 30 patients were assayed for anti-NP responses, for 'neutralisation' by the surrogate neutralisation assay and for neutralisation by SARS-CoV-2 S pseudotyped virus assays utilising two target cell lines. Correlation between assays was measured using linear regression. RESULTS: The responses observed within the surrogate neutralisation assay demonstrated an extremely strong, highly significant positive correlation with those observed in both pseudotyped virus assays. CONCLUSIONS: The tested ELISA-based surrogate assay provides an immunologically useful measure of functional immune responses in a much quicker and highly automatable fashion. It also reinforces that detection of anti-RBD neutralising antibodies alone is a powerful measure of the capacity to neutralise viral infection.
OBJECTIVES: To assess whether a commercially available CE-IVD, ELISA-based surrogate neutralisation assay (cPass, Genscript) provides a genuine measure of SARS-CoV-2 neutralisation by human sera, and further to establish whether measuring responses against the RBD of S was a diagnostically useful proxy for responses against the whole S protein. METHODS: Serum samples from 30 patients were assayed for anti-NP responses, for 'neutralisation' by the surrogate neutralisation assay and for neutralisation by SARS-CoV-2 S pseudotyped virus assays utilising two target cell lines. Correlation between assays was measured using linear regression. RESULTS: The responses observed within the surrogate neutralisation assay demonstrated an extremely strong, highly significant positive correlation with those observed in both pseudotyped virus assays. CONCLUSIONS: The tested ELISA-based surrogate assay provides an immunologically useful measure of functional immune responses in a much quicker and highly automatable fashion. It also reinforces that detection of anti-RBD neutralising antibodies alone is a powerful measure of the capacity to neutralise viral infection.
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