| Literature DB >> 33159009 |
Kevin W Ng1, Nikhil Faulkner1, Georgina H Cornish1, Annachiara Rosa2, Ruth Harvey3, Saira Hussain3, Rachel Ulferts4, Christopher Earl5, Antoni G Wrobel6, Donald J Benton6, Chloe Roustan7, William Bolland1, Rachael Thompson1, Ana Agua-Doce8, Philip Hobson8, Judith Heaney9, Hannah Rickman9, Stavroula Paraskevopoulou9, Catherine F Houlihan9,10, Kirsty Thomson9, Emilie Sanchez9, Gee Yen Shin9, Moira J Spyer9,11, Dhira Joshi12, Nicola O'Reilly12, Philip A Walker7, Svend Kjaer7, Andrew Riddell8, Catherine Moore13, Bethany R Jebson14,15, Meredyth Wilkinson14,15, Lucy R Marshall14,15, Elizabeth C Rosser14,16, Anna Radziszewska14,16, Hannah Peckham14,16, Coziana Ciurtin14,16, Lucy R Wedderburn14,15, Rupert Beale4, Charles Swanton17, Sonia Gandhi18, Brigitta Stockinger19, John McCauley3, Steve J Gamblin6, Laura E McCoy20, Peter Cherepanov21, Eleni Nastouli22,11, George Kassiotis23,24.
Abstract
Zoonotic introduction of novel coronaviruses may encounter preexisting immunity in humans. Using diverse assays for antibodies recognizing SARS-CoV-2 proteins, we detected preexisting humoral immunity. SARS-CoV-2 spike glycoprotein (S)-reactive antibodies were detectable using a flow cytometry-based method in SARS-CoV-2-uninfected individuals and were particularly prevalent in children and adolescents. They were predominantly of the immunoglobulin G (IgG) class and targeted the S2 subunit. By contrast, SARS-CoV-2 infection induced higher titers of SARS-CoV-2 S-reactive IgG antibodies targeting both the S1 and S2 subunits, and concomitant IgM and IgA antibodies, lasting throughout the observation period. SARS-CoV-2-uninfected donor sera exhibited specific neutralizing activity against SARS-CoV-2 and SARS-CoV-2 S pseudotypes. Distinguishing preexisting and de novo immunity will be critical for our understanding of susceptibility to and the natural course of SARS-CoV-2 infection.Entities:
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Year: 2020 PMID： 33159009 PMCID： PMC7857411 DOI： 10.1126/science.abe1107
Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728