Akouélé P Kuassi-Kpede1, Essolakina Dolou1, Théodora M Zohoncon1,2, Ina Marie Angèle Traore3,4, Gnatoulma Katawa5, R Alice Ouedraogo1,2, Esther Mah Traore1,2, Prosper Bado1,2, T Clarisse Ouedraogo1,2, Florencia Wendkuuni Djigma1,2, Simplice Damintoti Karou5, Jacques Simpore1,2. 1. Molecular Biology and Genetics Laboratory (LABIOGENE), Department of Biochemistry and Microbiology, University JOSEPH KI-ZERBO, P.O. Box 7021, Ouagadougou 03, Burkina Faso. 2. Pietro Annigoni Biomolecular Research Centre (CERBA), P.O. Box 364, Ouagadougou 01, Burkina Faso. 3. Molecular Biology and Genetics Laboratory (LABIOGENE), Department of Biochemistry and Microbiology, University JOSEPH KI-ZERBO, P.O. Box 7021, Ouagadougou 03, Burkina Faso. belima08@yahoo.fr. 4. Pietro Annigoni Biomolecular Research Centre (CERBA), P.O. Box 364, Ouagadougou 01, Burkina Faso. belima08@yahoo.fr. 5. Ecole Supérieure des Techniques Biologiques et Alimentaires (ESTBA-UL), University of Lomé, Lomé, Togo.
Abstract
BACKGROUND: The causative agent of cervical cancer referred to as Human papillomavirus (HPV) remains a real public health problem. Many countries in West Africa, such as Togo have no data on the high-risk HPV (HR-HPV) infection and genotypes distribution. In order to fill the knowledge gap in the field in Togo, the main objective of this study was to determine the prevalence of pre-cancerous lesions of the cervix and HR-HPV genotypes among Togolese women. METHODS: Samples were collected from 240 women by introducing a swab in the cervix. Then, the screening of precancerous cervical lesions using the visual inspection with acetic acid and lugol (VIA / VIL) was conducted. The HR-HPV genotypes were characterised by real-time multiplex PCR. RESULTS: Out of 240 women recruited, 128 (53.3%) were infected by HR-HPV. The most common genotypes were HPV 56 (22.7%), followed by HPV 51 (20.3%), HPV 31 (19.5%), HPV 52 (18.8%) and HPV 35 (17.2%). The least common genotypes were HPV 33 (2.3%) and HPV 16 (2.3%). Among the women, 1.3% (3/240) were positive to VIA/VIL. CONCLUSION: This study allowed HR-HPV genotypes to be characterised for the first time in Lomé, Togo. This will help in mapping the HR-HPV genotypes in West Africa.
BACKGROUND: The causative agent of cervical cancer referred to as Human papillomavirus (HPV) remains a real public health problem. Many countries in West Africa, such as Togo have no data on the high-risk HPV (HR-HPV) infection and genotypes distribution. In order to fill the knowledge gap in the field in Togo, the main objective of this study was to determine the prevalence of pre-cancerous lesions of the cervix and HR-HPV genotypes among Togolese women. METHODS: Samples were collected from 240 women by introducing a swab in the cervix. Then, the screening of precancerous cervical lesions using the visual inspection with acetic acid and lugol (VIA / VIL) was conducted. The HR-HPV genotypes were characterised by real-time multiplex PCR. RESULTS: Out of 240 women recruited, 128 (53.3%) were infected by HR-HPV. The most common genotypes were HPV 56 (22.7%), followed by HPV 51 (20.3%), HPV 31 (19.5%), HPV 52 (18.8%) and HPV 35 (17.2%). The least common genotypes were HPV 33 (2.3%) and HPV 16 (2.3%). Among the women, 1.3% (3/240) were positive to VIA/VIL. CONCLUSION: This study allowed HR-HPV genotypes to be characterised for the first time in Lomé, Togo. This will help in mapping the HR-HPV genotypes in West Africa.
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