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Literature DB >> 337104

Replication of plasmid pSC101 in Escherichia coli K12: requirement for dnaA function.

Abstract

Replication of pSC101 was analyzed by using DNA-DNA hybridization and alkaline sucrose gradient centrifugation. Mutants of the dnaA gene were tested for their capacity to replicate pSC101 DNA at a non-permissive temperature. Only a small amount of radioactive precursor was incorporated into pSC101 DNA in dnaA mutants at 42 degrees C whereas active incorporation into plasmid DNA took place in dnaA+ strain under the same conditions. The effect of the dnaA mutation was grater on plasmid DNA synthesis than on host chromosomal DNA synthesis. The numbers of copies of pSC101 per chromosome in wild type and dnaA strains, grown at 30 degrees C, were about 8 and 2, respectively. These results indicate that the dna A gene product is required for the replication of pSC101 DNA.

Entities: Chemical Species

Mesh：

Substances：
DNA, Bacterial

Year: 1977 PMID： 337104 DOI： 10.1007/bf00571277

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

15 in total

1. Construction of biologically functional bacterial plasmids in vitro.

Authors: S N Cohen; A C Chang; H W Boyer; R B Helling
Journal: Proc Natl Acad Sci U S A Date: 1973-11 Impact factor: 11.205

2. Sensitivity of Escherichia coli to viral nucleic acid. VI. Capacity of dna mutants and DNA polymerase-less mutants for multiplication of phiA and phiX 174.

Authors: A Taketo
Journal: Mol Gen Genet Date: 1973-03-27

Replication of plasmid pSC101 in Escherichia coli K12: requirement for dnaA function.

1. Construction of biologically functional bacterial plasmids in vitro.

2. Sensitivity of Escherichia coli to viral nucleic acid. VI. Capacity of dna mutants and DNA polymerase-less mutants for multiplication of phiA and phiX 174.

3. General method for the isolation of plasmid deoxyribonucleic acid.

4. A quantitative assay for DNA-DNA hybrids using membrane filters.

5. Indirect selection of bacterial plasmids lacking identifiable phenotypic properties.

6. Plasmid ColE1 DNA replication in Escherichia coli strains temperature-sensitive for DNA replication.

7. Escherichia coli mutants temperature-sensitive for DNA synthesis.

8. A membrane-filter technique for the detection of complementary DNA.

9. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.

10. Conversion of phiX174 viral DNA to double-stranded form by purified Escherichia coli proteins.

1. Mechanistic aspects of DnaA-RepA interaction as revealed by yeast forward and reverse two-hybrid analysis.

2. Replication origin mutations affecting binding of pSC101 plasmid-encoded Rep initiator protein.

3. New pSC101-derivative cloning vectors with elevated copy numbers.

4. Novel alleles of the Escherichia coli dnaA gene are defective in replication of pSC101 but not of oriC.

Review 5. Replication and control of circular bacterial plasmids.

6. Primary structure of the essential replicon of the plasmid pSC101.

7. In vivo and in vitro studies of a copy number mutation of the RepA replication protein of plasmid pSC101.

8. Effect of dna mutations on the replication of plasmid pSC101 in Escherichia coli K-12.

9. Host participation in plasmid maintenance: dependence upon dnaA of replicons derived from P1 and F.

10. Replication of pSC101: effects of mutations in the E. coli DNA binding protein IHF.