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Literature DB >> 7592447

Novel alleles of the Escherichia coli dnaA gene are defective in replication of pSC101 but not of oriC.

Abstract

Five novel alleles of the Escherichia coli dnaA gene that were temperature sensitive in maintenance of pSC101, a plasmid that is dependent on this gene for replication, were isolated. Nucleotide sequence analysis revealed that four of the five alleles arose from single base substitutions, whereas the fifth contained three base substitutions, two of which were silent. Whereas all five alleles were temperature sensitive in vivo for pSC101 maintenance, genetic and biochemical characterization indicated that only two were defective in replication from the chromosomal origin, oriC. As previously characterized mutations are defective in replication for both pSC101 and oriC, the dnaA mutations specifically defective in pSC101 maintenance represent a novel class. We speculate that one or more of these pSC101-specific mutants are defective in interaction with pSC101 RepA protein, which is also required for initiation of plasmid DNA replication.

Entities: Gene Species

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Year: 1995 PMID： 7592447 PMCID： PMC177522 DOI： 10.1128/jb.177.22.6657-6665.1995

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

52 in total

1. Reinitiation of chromosome replication in the presence of chloramphenicol under an integratively suppressed state by R6K.

Authors: M Sotomura; M Yoshikawa
Journal: J Bacteriol Date: 1975-05 Impact factor: 3.490

2. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors: M M Bradford
Journal: Anal Biochem Date: 1976-05-07 Impact factor: 3.365

3. The effects of an Escherichia coli dnaAts mutation on the replication of the plasmids colE1 pSC101, R100.1 and RTF-TC.

Authors: J Frey; M Chandler; L Caro
Journal: Mol Gen Genet Date: 1979-07-13

4. Revised interpretation of the origin of the pSC101 plasmid.

Authors: S N Cohen; A C Chang
Journal: J Bacteriol Date: 1977-11 Impact factor: 3.490

5. Chromosome replication in Escherichia coli. IV. Control of chromosome replication and cell division by an integrated episome.

Authors: Y Nishimura; L Caro; C M Berg; Y Hirota
Journal: J Mol Biol Date: 1971-02-14 Impact factor: 5.469

10. DpiA binding to the replication origin of Escherichia coli plasmids and chromosomes destabilizes plasmid inheritance and induces the bacterial SOS response.

Authors: Christine Miller; Hanne Ingmer; Line Elnif Thomsen; Kirsten Skarstad; Stanley N Cohen
Journal: J Bacteriol Date: 2003-10 Impact factor: 3.490

Novel alleles of the Escherichia coli dnaA gene are defective in replication of pSC101 but not of oriC.

1. Reinitiation of chromosome replication in the presence of chloramphenicol under an integratively suppressed state by R6K.

2. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

3. The effects of an Escherichia coli dnaAts mutation on the replication of the plasmids colE1 pSC101, R100.1 and RTF-TC.

4. Revised interpretation of the origin of the pSC101 plasmid.

5. Chromosome replication in Escherichia coli. IV. Control of chromosome replication and cell division by an integrated episome.

6. Thermosensitive mutants of E. coli affected in the processes of DNA synthesis and cellular division.

7. Genetic code: the 'nonsense' triplets for chain termination and their suppression.

8. [Thermosensitive mutants of Escherichia coli K 12. I. Isolation and rapid characterization].

9. Reversibility of DnaA protein activity in the 'irreversible' dnaA204 mutant of Escherichia coli.

10. Evidence of two levels of control of P1 oriR and host oriC replication origins by DNA adenine methylation.

1. Mechanistic aspects of DnaA-RepA interaction as revealed by yeast forward and reverse two-hybrid analysis.

2. The structure of bacterial DnaA: implications for general mechanisms underlying DNA replication initiation.

3. The Escherichia coli dnaN159 mutant displays altered DNA polymerase usage and chronic SOS induction.

4. Primosomal proteins DnaD and DnaB are recruited to chromosomal regions bound by DnaA in Bacillus subtilis.

Review 5. Linkage map of Escherichia coli K-12, edition 10: the traditional map.

6. Separate roles of Escherichia coli replication proteins in synthesis and partitioning of pSC101 plasmid DNA.

7. HspQ Functions as a Unique Specificity-Enhancing Factor for the AAA+ Lon Protease.

8. Escherichia coli DnaA forms helical structures along the longitudinal cell axis distinct from MreB filaments.

9. Mechanism of recruitment of DnaB helicase to the replication origin of the plasmid pSC101.

10. DpiA binding to the replication origin of Escherichia coli plasmids and chromosomes destabilizes plasmid inheritance and induces the bacterial SOS response.