Literature DB >> 33659521

Assembly of Genetic Circuits with the Mammalian ToolKit.

João P Fonseca1, Alain R Bonny1, Jason Town1, Hana El-Samad1,2.   

Abstract

The ability to rapidly assemble and prototype cellular circuits is vital for biological research and its applications in biotechnology and medicine. The Mammalian ToolKit (MTK) is a Golden Gate-based cloning toolkit for fast, reproducible and versatile assembly of large DNA vectors and their implementation in mammalian models. The MTK consists of a curated library of characterized, modular parts that can be assembled into transcriptional units and further weaved into complex circuits. These circuits are easily repurposed and introduced in mammalian cells by different methods.
Copyright © 2020 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  Cloning; Golden-gate; High-throughput; Library; Mammalian; Toolkit

Year:  2020        PMID： 33659521      PMCID： PMC7842717          DOI： 10.21769/BioProtoc.3547

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  10 in total

1.  A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly.

Authors:  Michael E Lee; William C DeLoache; Bernardo Cervantes; John E Dueber
Journal:  ACS Synth Biol       Date:  2015-05-01       Impact factor: 5.110

2.  Loop assembly: a simple and open system for recursive fabrication of DNA circuits.

Authors:  Bernardo Pollak; Ariel Cerda; Mihails Delmans; Simón Álamos; Tomás Moyano; Anthony West; Rodrigo A Gutiérrez; Nicola J Patron; Fernán Federici; Jim Haseloff
Journal:  New Phytol       Date:  2019-02-25       Impact factor: 10.151

3.  Single Day Construction of Multigene Circuits with 3G Assembly.

Authors:  Andrew D Halleran; Anandh Swaminathan; Richard M Murray
Journal:  ACS Synth Biol       Date:  2018-05-07       Impact factor: 5.110

4.  A Toolkit for Rapid Modular Construction of Biological Circuits in Mammalian Cells.

Authors:  João Pedro Fonseca; Alain R Bonny; G Renuka Kumar; Andrew H Ng; Jason Town; Qiu Chang Wu; Elham Aslankoohi; Susan Y Chen; Galen Dods; Patrick Harrigan; Lindsey C Osimiri; Amy L Kistler; Hana El-Samad
Journal:  ACS Synth Biol       Date:  2019-11-05       Impact factor: 5.110

5.  EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid Design and Production of Diverse Expression Vectors.

Authors:  Andrea Martella; Mantas Matjusaitis; Jamie Auxillos; Steven M Pollard; Yizhi Cai
Journal:  ACS Synth Biol       Date:  2017-04-24       Impact factor: 5.110

6.  Assembly of designer TAL effectors by Golden Gate cloning.

Authors:  Ernst Weber; Ramona Gruetzner; Stefan Werner; Carola Engler; Sylvestre Marillonnet
Journal:  PLoS One       Date:  2011-05-19       Impact factor: 3.240

7.  A modular cloning system for standardized assembly of multigene constructs.

Authors:  Ernst Weber; Carola Engler; Ramona Gruetzner; Stefan Werner; Sylvestre Marillonnet
Journal:  PLoS One       Date:  2011-02-18       Impact factor: 3.240

8.  A platform for rapid prototyping of synthetic gene networks in mammalian cells.

Authors:  Xavier Duportet; Liliana Wroblewska; Patrick Guye; Yinqing Li; Justin Eyquem; Julianne Rieders; Tharathorn Rimchala; Gregory Batt; Ron Weiss
Journal:  Nucleic Acids Res       Date:  2014-11-05       Impact factor: 16.971

9.  Adaptation of the GoldenBraid modular cloning system and creation of a toolkit for the expression of heterologous proteins in yeast mitochondria.

Authors:  Ana Pérez-González; Ryan Kniewel; Marcel Veldhuizen; Hemant K Verma; Mónica Navarro-Rodríguez; Luis M Rubio; Elena Caro
Journal:  BMC Biotechnol       Date:  2017-11-13       Impact factor: 2.563

10.  A Modular Assembly Platform for Rapid Generation of DNA Constructs.

Authors:  Elliot H Akama-Garren; Nikhil S Joshi; Tuomas Tammela; Gregory P Chang; Bethany L Wagner; Da-Yae Lee; William M Rideout; Thales Papagiannakopoulos; Wen Xue; Tyler Jacks
Journal:  Sci Rep       Date:  2016-02-18       Impact factor: 4.379
  10 in total

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