| Literature DB >> 33613873 |
Monika Pietrowska1, Aneta Zebrowska1, Marta Gawin1, Lukasz Marczak2, Priyanka Sharma3, Sujan Mondal3, Justyna Mika4, Joanna Polańska4, Soldano Ferrone5, John M Kirkwood3,6, Piotr Widlak1, Theresa L Whiteside3,7.
Abstract
Molecular profiling of small extracellular vesicles (sEV) isolated from plasma of cancer patients emerges as promising strategy for biomarkers discovery. We investigated the proteomic profiles of sEV immunoselected using anti-CSPG4 antibodies from 15 melanoma patients' plasma. The proteomes of sEV separated into melanoma cell-derived (MTEX) and non-malignant cell-derived (NMTEX) were compared using high-resolution mass spectrometry. Paired analysis identified the MTEX-associated profile of 16 proteins that discriminated MTEX from NMETEX. We also identified the MTEX profile that discriminated between seven patients with no evidence of melanoma (NED) after therapy and eight with progressive disease (PD). Among 75 MTEX proteins overexpressed in PD patients, PDCD6IP (ALIX) had the highest discriminating value, while CNTN1 (contactin-1) was upregulated only in MTEX of NED patients. This is the first report documenting that proteomes of tumour-derived sEV in patients' plasma discriminate cancer from non-cancer and identify proteins with potential to serve as prognostic biomarkers in melanoma.Entities:
Keywords: high‐resolution mass spectrometry (HRMS); melanoma cell‐derived exosomes (MTEX); proteomics; small extracellular vesicles (sEV); tumour‐derived exosomes (TEX)
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Year: 2021 PMID： 33613873 PMCID： PMC7876545 DOI： 10.1002/jev2.12063
Source DB: PubMed Journal: J Extracell Vesicles ISSN： 2001-3078