Nadezhda T Zhilinskaya1,2, Vladimir G Bespalov3,4, Alexander L Semenov3, Elena D Ermakova3,5, Grigory V Tochilnikov3, Nadezhda V Barakova4, Valerii A Alexandrov3, Denis A Baranenko4. 1. Department of Cancer Chemoprevention and Oncopharmacology, N. N. Petrov National Medical Research Center of Oncology, 68, Leningradskaya Ul., Pesochnyi, St. Petersburg, 197758, Russia. jilinskie@mail.ru. 2. Graduate School of Biotechnologies and Food Science, Institute of Biomedical Systems and Biotechnologies, Peter the Great St. Petersburg Polytechnic University, 29, Polytekhnicheskaya, St. Petersburg, 195251, Russia. jilinskie@mail.ru. 3. Department of Cancer Chemoprevention and Oncopharmacology, N. N. Petrov National Medical Research Center of Oncology, 68, Leningradskaya Ul., Pesochnyi, St. Petersburg, 197758, Russia. 4. International Research Centre "Biotechnologies of the Third Millennium", ITMO University, 49, Kronversky Pr, St. Petersburg, 197101, Russia. 5. Graduate School of Biotechnologies and Food Science, Institute of Biomedical Systems and Biotechnologies, Peter the Great St. Petersburg Polytechnic University, 29, Polytekhnicheskaya, St. Petersburg, 195251, Russia.
Abstract
BACKGROUND: Ovarian cancer is one of the most common diseases of the female reproductive system. The aim of this study was the investigation of the antitumor cisplatin effect on ascitic fluid tumor cells in the ovarian cancer experimental model by digital cytomorphometry and cell bioinformatic analysis. METHODS: Female Wistar rats were inoculated by ovarian cancer strain. After ovarian cancer transplantation rats were divided into two groups: control group-without cisplatin treatment, the experimental group-under cisplatin treatment. The ascitic fluid was taken on the 9-th day after tumor cell inoculation. Digital cytomorphometric and cytobioinformatic analysis were used to study ascitic fluid cancer cell morphofunctional changes under cisplatin treatment. RESULTS: Digital cytomorphometric characteristics of rat ovarian cancer ascitic cells were obtained. Tumor cells were classified into four groups according to their geometric size: small, medium, large, "gigantic". The algorithm and the computer program based on tumor cell morphometric characteristics were created to calculate such cell bioinformatic characteristic as information redundancy coefficient R. Three parameters were determined as the criteria of cisplatin effect on cancer cells: cell number, nuclear/cytoplasmic ratio, R-value. CONCLUSIONS: Data obtained suggest that cisplatin reduces the total cell number, the nuclear/cytoplasmic ratio and R-value thus indicates a decrease in cellular resistance and adaptive potential. The digital cytomorphometry and bioinformatics could be recommended as a testing system in the experimental or clinical study.
BACKGROUND:Ovarian cancer is one of the most common diseases of the female reproductive system. The aim of this study was the investigation of the antitumor cisplatin effect on ascitic fluid tumor cells in the ovarian cancer experimental model by digital cytomorphometry and cell bioinformatic analysis. METHODS: Female Wistar rats were inoculated by ovarian cancer strain. After ovarian cancer transplantation rats were divided into two groups: control group-without cisplatin treatment, the experimental group-under cisplatin treatment. The ascitic fluid was taken on the 9-th day after tumor cell inoculation. Digital cytomorphometric and cytobioinformatic analysis were used to study ascitic fluid cancer cell morphofunctional changes under cisplatin treatment. RESULTS: Digital cytomorphometric characteristics of ratovarian cancer ascitic cells were obtained. Tumor cells were classified into four groups according to their geometric size: small, medium, large, "gigantic". The algorithm and the computer program based on tumor cell morphometric characteristics were created to calculate such cell bioinformatic characteristic as information redundancy coefficient R. Three parameters were determined as the criteria of cisplatin effect on cancer cells: cell number, nuclear/cytoplasmic ratio, R-value. CONCLUSIONS: Data obtained suggest that cisplatin reduces the total cell number, the nuclear/cytoplasmic ratio and R-value thus indicates a decrease in cellular resistance and adaptive potential. The digital cytomorphometry and bioinformatics could be recommended as a testing system in the experimental or clinical study.
Entities:
Keywords:
Apoptosis; Cell bioinformatics; Cisplatin; Digital cytomorphometry; Experimental ovarian cancer
Authors: Cynthia C Nast; Kevin V Lemley; Jeffrey B Hodgin; Serena Bagnasco; Carmen Avila-Casado; Stephen M Hewitt; Laura Barisoni Journal: Semin Nephrol Date: 2015-05 Impact factor: 5.299
Authors: G Kroemer; W S El-Deiry; P Golstein; M E Peter; D Vaux; P Vandenabeele; B Zhivotovsky; M V Blagosklonny; W Malorni; R A Knight; M Piacentini; S Nagata; G Melino Journal: Cell Death Differ Date: 2005-11 Impact factor: 15.828
Authors: A A Zhikhoreva; A V Belashov; V G Bespalov; A L Semenov; I V Semenova; G V Tochilnikov; N T Zhilinskaya; O S Vasyutinskii Journal: Biomed Opt Express Date: 2018-10-29 Impact factor: 3.732
Authors: Vladimir G Bespalov; Ivan K Alvovsky; Grigory V Tochilnikov; Alexandr N Stukov; Ekaterina A Vyshinskaya; Alexandr L Semenov; Irina N Vasilyeva; Olesya A Belyaeva; Galina S Kireeva; Konstantin Y Senchik; Nadezhda T Zhilinskaya; Julia D Von; Larisa A Krasilnikova; Valery A Alexandrov; Nikita N Khromov-Borisov; Denis A Baranenko; Alexey M Belyaev Journal: Int J Hyperthermia Date: 2017-09-18 Impact factor: 3.914