Sujesh Sudarsan1, Madan Kumar Shankar2, Anil Kumar Belagal Motatis1, Sushmitha Shankar1, Darshan Krishnappa1, Chakrabhavi Dhananjaya Mohan3, Kanchugarakoppal S Rangappa4, Vijai Kumar Gupta5,6, Chandra Nayaka Siddaiah1. 1. Department of Studies in Biotechnology, University of Mysore, Manasagangotri, Mysore-570006, India. 2. Institute of Excellence, VijnanaBhavan, University of Mysore, Manasagangotri, Mysore-570006, India. 3. Department of Studies in Molecular Biology, University of Mysore, Manasagangotri, Mysore-570006, India. 4. Department of Studies in Chemistry, University of Mysore, Manasagangotri, Mysore-570006, India. 5. Center for Safe and Improved Food, Scotland's Rural College (SRUC), Kings Buildings, West Mains Road, Edinburgh EH9 3JG, UK. 6. Biorefining and Advanced Materials Research Center, Scotland's Rural College (SRUC), Kings Buildings, West Mains Road, Edinburgh EH9 3JG, UK.
Abstract
This work reports an eco-friendly synthesis of silver nanoparticles (AgNPs) using endophytic bacteria, Cytobacillus firmus isolated from the stem bark of Terminalia arjuna. The synthesis of AgNPs was confirmed by visual observation as a change in color of the bacterial solution impregnated with silver. Further, the morphology of the AgNPs, average size, and presence of elemental silver were characterized by UV-Visible spectroscopy, scanning electron microscopy, and dynamic light scattering spectroscopy. The roles of endophytic secondary metabolites in the metal reduction, stabilization, and capping of silver nanoparticles were studied by qualitative FTIR spectral peaks. The antimicrobial ability of AgNPs was evaluated against Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria and pearl millet blast disease-causing fungi (Magnoporthe grisea). The biosynthesized AgNPs showed good antibacterial and antifungal activities. AgNPs effectively inhibited the bacterial growth in a dose-dependent manner and presented as good antifungal agents towards the growth of Magnoporthe grisea.
This work reports an eco-friendly synthesis of silvernanoparticles (n class="Chemical">AgNPs) using endophytic bacteria, Cytobacillus firmus isolated from the stem bark of Terminalia arjuna. The synthesis of AgNPs was confirmed by visual observation as a change in color of the bacterial solution impregnated with silver. Further, the morphology of the AgNPs, average size, and presence of elemental silver were characterized by UV-Visible spectroscopy, scanning electron microscopy, and dynamic light scattering spectroscopy. The roles of endophytic secondary metabolites in the metal reduction, stabilization, and capping of silver nanoparticles were studied by qualitative FTIR spectral peaks. The antimicrobial ability of AgNPs was evaluated against Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria and pearl millet blast disease-causing fungi (Magnoporthe grisea). The biosynthesized AgNPs showed good antibacterial and antifungal activities. AgNPs effectively inhibited the bacterial growth in a dose-dependent manner and presented as good antifungal agents towards the growth of Magnoporthe grisea.
Authors: Guangquan Li; Dan He; Yongqing Qian; Buyuan Guan; Song Gao; Yan Cui; Koji Yokoyama; Li Wang Journal: Int J Mol Sci Date: 2011-12-29 Impact factor: 5.923
Authors: Fazilath Uzma; Chakrabhavi D Mohan; Abeer Hashem; Narasimha M Konappa; Shobith Rangappa; Praveen V Kamath; Bhim P Singh; Venkataramana Mudili; Vijai K Gupta; Chandra N Siddaiah; Srinivas Chowdappa; Abdulaziz A Alqarawi; Elsayed F Abd Allah Journal: Front Pharmacol Date: 2018-04-26 Impact factor: 5.810
Authors: Noor Ul Huda Altaf; Muhammad Yasin Naz; Shazia Shukrullah; Madiha Ghamkhar; Muhammad Irfan; Saifur Rahman; Tomasz Jakubowski; Esam A Alqurashi; Adam Glowacz; Mater H Mahnashi Journal: Materials (Basel) Date: 2022-05-27 Impact factor: 3.748