Johnny S Younis1,2, Mariana Issawy3, Ido Izhaki4, Izhar Ben-Shlomo3, Sofia Soltsman3, Yuri Perlitz3,5, Moshe Ben-Ami3,5, Benjamin Brenner6,7, Galit Sarig8,7. 1. Reproductive Medicine, Department of Obstetrics and Gynecology, Baruch Padeh Medical Center, 15208, Poriya, Israel. jyounis@poria.health.gov.il. 2. Azrielli Faculty of Medicine in Galilee, Bar-Ilan University, Safed, Israel. jyounis@poria.health.gov.il. 3. Reproductive Medicine, Department of Obstetrics and Gynecology, Baruch Padeh Medical Center, 15208, Poriya, Israel. 4. Department of Evolutionary and Environmental Biology, University of Haifa, Haifa, Israel. 5. Azrielli Faculty of Medicine in Galilee, Bar-Ilan University, Safed, Israel. 6. Thrombosis and Hemostasis Unit, Department of Hematology and Bone Marrow Transplantation, Rambam Health Care Campus, Haifa, Israel. 7. Bruce Rappaport Faculty of Medicine, The Technion-Israel Institute of Technology, Haifa, Israel. 8. Hematology Laboratory, Rambam Health Care Campus, Haifa, Israel.
Abstract
PURPOSE: Protein C global assay tests the global function of the protein C pathway, the most clinically significant anticoagulant pathway in humans. The objective of this study is to assess the difference in protein C global assay levels, throughout twin gestation, in naturally conceiving and ART-treated women. METHODS: This is a prospective cohort longitudinal study of pregnant women with twin gestation. Protein C Global evaluation was performed on frozen blood samples. Ninety-eight women with twin pregnancy, thirty-eight naturally conceived and sixty following ART, were evaluated on four occasions: during the first, second, and third trimesters, and 6 weeks or later after delivery (baseline). RESULTS: Protein C global assay levels were lower throughout pregnancy as compared to basal levels in both the naturally conceived and ART-conceived groups. However, protein C global assay levels were similar between the ART-conceived and naturally conceived twin pregnancies in all three trimesters. Perinatal complications were associated with decreased protein C global assay levels during the third trimester, although no difference was encountered between naturally conceived and ART-complicated twin pregnancies. CONCLUSION: While protein C global assay levels drop during twin pregnancy, there is no difference between ART-conceived and naturally conceived gestations. Decreased levels of protein C global assay during the third trimester were similarly associated with perinatal complications in both groups. Our results imply that twin pregnancy of itself is a more dominant factor for perinatal complications as compared to other factors, such as subfertility or the exposure to ART per se.
PURPOSE: Protein C global assay tests the global function of the protein C pathway, the most clinically significant anticoagulant pathway in humans. The objective of this study is to assess the difference in protein C global assay levels, throughout twin gestation, in naturally conceiving and ART-treated women. METHODS: This is a prospective cohort longitudinal study of pregnant women with twin gestation. Protein C Global evaluation was performed on frozen blood samples. Ninety-eight women with twin pregnancy, thirty-eight naturally conceived and sixty following ART, were evaluated on four occasions: during the first, second, and third trimesters, and 6 weeks or later after delivery (baseline). RESULTS: Protein C global assay levels were lower throughout pregnancy as compared to basal levels in both the naturally conceived and ART-conceived groups. However, protein C global assay levels were similar between the ART-conceived and naturally conceived twin pregnancies in all three trimesters. Perinatal complications were associated with decreased protein C global assay levels during the third trimester, although no difference was encountered between naturally conceived and ART-complicated twin pregnancies. CONCLUSION: While protein C global assay levels drop during twin pregnancy, there is no difference between ART-conceived and naturally conceived gestations. Decreased levels of protein C global assay during the third trimester were similarly associated with perinatal complications in both groups. Our results imply that twin pregnancy of itself is a more dominant factor for perinatal complications as compared to other factors, such as subfertility or the exposure to ART per se.
Entities:
Keywords:
Assisted reproductive technologies; Protein C global assay; Subfertility; Twin pregnancy
Authors: E M Faioni; G Fontana; C Razzari; L Avagliano; G Bulfamante; E Calvi; P Doi; A M Marconi Journal: Reprod Sci Date: 2015-02-08 Impact factor: 3.060
Authors: D Caserta; G Bordi; M Stegagno; F Filippini; M Podagrosi; D Roselli; M Moscarini Journal: Eur J Obstet Gynecol Reprod Biol Date: 2013-12-15 Impact factor: 2.435
Authors: Elisabet Clua; Rosa Tur; Buenaventura Coroleu; Montse Boada; I Rodríguez; Pedro N Barri; Anna Veiga Journal: Reprod Biomed Online Date: 2012-09-21 Impact factor: 3.828
Authors: Minna E Geisler; Anne O'Mahony; Sarah Meaney; John J Waterstone; Keelin O'Donoghue Journal: Eur J Obstet Gynecol Reprod Biol Date: 2014-08-01 Impact factor: 2.435