Ning Yang1, Jingxin Peng1, Liang Wu2, Xue Han1, Naila Shaheen1, Xiaobo Zou3. 1. Department of Electrical and Information EngineeringJiangsu UniversityZhenjiang212013China. 2. Department of MedicineJiangsu UniversityZhenjiang212013China. 3. Department of Food and Biological EngineeringJiangsu UniversityZhenjiang212013China.
Abstract
BACKGROUND: Vaginitis is a common and very private disease, and the current diagnosis is a frequent go to the hospital for testing. OBJECTIVE: In order to improve the convenience and speed of detection, in this paper, we have developed a hand-held zoom micro-imaging system based on a microfluidic chip for point-of-care testing (POCT) of vaginal inflammation. METHODS: This system consists of a microfluidic chip, an optical system and a hand-held zoom system assembled with a mobile phone. In terms of hardware, we designed a self-priming microfluidic chip, which can realize automatic sampling and full mixing of samples. We have also developed an optical system that can be adapted to smartphones, which has a lens group with a 37x magnification function and equipped with a zoom system with a focus range of 4mm to 6mm. In terms of software, we proposed an APP that can accurately identify cocci and can determine the inflammation level. RESULTS: Compared with the recognition rate of the observers in the hospital, the detection accuracy of the portable recognition system is 95%, and after testing the clinical samples, the results were completely consistent with the hospital diagnosis results. The detection limit was 500 CFU / ml, which the relative error was (0.9 ± 0.3) %, and recognition time is 7 seconds. CONCLUSION: This system is definitely suitable for women's point-of-care testing (POCT).
BACKGROUND: Vaginitis is a common and very private disease, and the current diagnosis is a frequent go to the hospital for testing. OBJECTIVE: In order to improve the convenience and speed of detection, in this paper, we have developed a hand-held zoom micro-imaging system based on a microfluidic chip for point-of-care testing (POCT) of vaginal inflammation. METHODS: This system consists of a microfluidic chip, an optical system and a hand-held zoom system assembled with a mobile phone. In terms of hardware, we designed a self-priming microfluidic chip, which can realize automatic sampling and full mixing of samples. We have also developed an optical system that can be adapted to smartphones, which has a lens group with a 37x magnification function and equipped with a zoom system with a focus range of 4mm to 6mm. In terms of software, we proposed an APP that can accurately identify cocci and can determine the inflammation level. RESULTS: Compared with the recognition rate of the observers in the hospital, the detection accuracy of the portable recognition system is 95%, and after testing the clinical samples, the results were completely consistent with the hospital diagnosis results. The detection limit was 500 CFU / ml, which the relative error was (0.9 ± 0.3) %, and recognition time is 7 seconds. CONCLUSION: This system is definitely suitable for women's point-of-care testing (POCT).
Authors: Chhavi Goenka; William Lewis; Lee Roger Chevres-Fernández; Antonio Ortega-Martínez; Esmeralda Ibarra-Silva; Maura Williams; Walfre Franco Journal: Lasers Surg Med Date: 2018-08-16 Impact factor: 4.025