| Literature DB >> 33547303 |
Johan Quilbé1, Léo Lamy1,2, Laurent Brottier1, Philippe Leleux1,2, Joël Fardoux1, Ronan Rivallan3,4, Thomas Benichou1, Rémi Guyonnet1, Manuel Becana5, Irene Villar5, Olivier Garsmeur3,4, Bárbara Hufnagel6, Amandine Delteil1, Djamel Gully1, Clémence Chaintreuil1, Marjorie Pervent1, Fabienne Cartieaux1, Mickaël Bourge7, Nicolas Valentin7, Guillaume Martin3,4, Loïc Fontaine8, Gaëtan Droc3,4, Alexis Dereeper9, Andrew Farmer10, Cyril Libourel11, Nico Nouwen1, Frédéric Gressent1, Pierre Mournet3,4, Angélique D'Hont3,4, Eric Giraud1, Christophe Klopp2, Jean-François Arrighi12.
Abstract
Among legumes (Fabaceae) capable of nitrogen-fixing nodulation, several Aeschynomene spp. use a unique symbiotic process that is independent of Nod factors and infection threads. They are also distinctive in developing root and stem nodules with photosynthetic bradyrhizobia. Despite the significance of these symbiotic features, their understanding remains limited. To overcome such limitations, we conduct genetic studies of nodulation in Aeschynomene evenia, supported by the development of a genome sequence for A. evenia and transcriptomic resources for 10 additional Aeschynomene spp. Comparative analysis of symbiotic genes substantiates singular mechanisms in the early and late nodulation steps. A forward genetic screen also shows that AeCRK, coding a receptor-like kinase, and the symbiotic signaling genes AePOLLUX, AeCCamK, AeCYCLOPS, AeNSP2, and AeNIN are required to trigger both root and stem nodulation. This work demonstrates the utility of the A. evenia model and provides a cornerstone to unravel mechanisms underlying the rhizobium-legume symbiosis.Entities:
Year: 2021 PMID： 33547303 PMCID： PMC7864950 DOI： 10.1038/s41467-021-21094-7
Source DB: PubMed Journal: Nat Commun ISSN： 2041-1723 Impact factor: 14.919