Shimaa A Amer1, Wafaa A M Mohamed2, Heba S A Gharib3, Naif A Al-Gabri4, Ahmed Gouda5, Mohamed Tharwat Elabbasy6,7, Ghada I Abd El-Rahman2, Anaam E Omar8. 1. Department of Nutrition & Clinical Nutrition, Faculty of Veterinary Medicine, Zagazig University, Zagazig, 44511, Egypt. shimaa.amer@zu.edu.eg. 2. Department of Clinical Pathology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, 44511, Egypt. 3. Department of Veterinary Public Health, Faculty of Veterinary Medicine, Zagazig University, Zagazig, 44511, Egypt. 4. Pathology Department, Faculty of Veterinary Medicine, Thamar University, Dahamar, Yemen. 5. Department of Animal Production, National Research Centre, Dokki, 12622, Egypt. 6. College of Public Health and Molecular Diagnostics and Personalized Therapeutics Center (CMDPT) Hail University, Hail, 2440, Saudi Arabia. 7. Food Control Department, Faculty of Veterinary Medicine, Zagazig University, Zagazig, 44519, Egypt. 8. Department of Nutrition & Clinical Nutrition, Faculty of Veterinary Medicine, Zagazig University, Zagazig, 44511, Egypt.
Abstract
BACKGROUND: The effects of safflower oil and vitamin C (Vit. C) inclusion in broiler chicken diets on the growth performance, apparent ileal digestibility coefficient "AID%" of amino acids, intestinal histology, behavior, carcass traits, fatty acid composition of the breast muscle, antioxidant and immune status for a 35-day feeding period were evaluated. A total of 300 three-day-old Ross chicks (58.25 g ± 0.19) were randomly allotted in a 2 × 3 factorial design consisting of two levels of vitamin C (0 and 400 mg/kg diet) and three levels of safflower oil (0, 5, and 10 g/kg diet). RESULTS: An increase in the final body weight, total body weight gain, total feed intake, and the relative growth rate (P < 0.05) were reported by safflower oil and vitamin C inclusion. Dietary supplementation of safflower oil and vitamin C had a positive effect (P < 0.05) on the ingestive, resting, and feather preening behavior. Vitamin C supplementation increased (P < 0.05) the AID% of lysine, threonine, tryptophan, arginine, and valine. Safflower inclusion (10 g/kg) increased (P < 0.05) the AID% of methionine and isoleucine. Safflower oil inclusion increased (P < 0.05) the levels of stearic acid, linoleic acid, saturated fatty acids, and omega-3 fatty acids (ω-3) in the breast muscle. In contrast, the supplementation of only 10 g of safflower oil/kg diet increased (P = 0.01) the omega-3/omega-6 (ω-3/ω-6) fatty acids ratio. Vit. C supplementation increased (P < 0.05) the CAT serum levels, SOD, and GSH enzymes. Dietary supplementation of safflower oil and vitamin C improved the intestinal histology. They increased the villous height and width, crypt depth, villous height/crypt depth ratio, mucosal thickness, goblet cell count, and intra-epithelium lymphocytic lick cell infiltrations. The serum levels of IgA and complement C3 were increased (P < 0.01) by Vit. C supplementation and prominent in the 400 vit. C + 10 safflower Oil group. CONCLUSION: A dietary combination of safflower oil and vitamin C resulted in improved growth rate, amino acids AID%, intestinal histology, welfare, immune and antioxidant status of birds, and obtaining ω-3 and linoleic acid-enriched breast muscles. The best inclusion level was 400 vit. C + 10 safflower Oil.
BACKGROUND: The effects of saffloweroil and vitamin C (Vit. C) inclusion in broiler chicken diets on the growth performance, apparent ileal digestibility coefficient "AID%" of amino acids, intestinal histology, behavior, carcass traits, fatty acid composition of the breast muscle, antioxidant and immune status for a 35-day feeding period were evaluated. A total of 300 three-day-old Ross chicks (58.25 g ± 0.19) were randomly allotted in a 2 × 3 factorial design consisting of two levels of vitamin C (0 and 400 mg/kg diet) and three levels of saffloweroil (0, 5, and 10 g/kg diet). RESULTS: An increase in the final body weight, total body weight gain, total feed intake, and the relative growth rate (P < 0.05) were reported by saffloweroil and vitamin C inclusion. Dietary supplementation of saffloweroil and vitamin C had a positive effect (P < 0.05) on the ingestive, resting, and feather preening behavior. Vitamin C supplementation increased (P < 0.05) the AID% of lysine, threonine, tryptophan, arginine, and valine. Safflower inclusion (10 g/kg) increased (P < 0.05) the AID% of methionine and isoleucine. Saffloweroil inclusion increased (P < 0.05) the levels of stearic acid, linoleic acid, saturated fatty acids, and omega-3 fatty acids (ω-3) in the breast muscle. In contrast, the supplementation of only 10 g of saffloweroil/kg diet increased (P = 0.01) the omega-3/omega-6 (ω-3/ω-6) fatty acids ratio. Vit. C supplementation increased (P < 0.05) the CAT serum levels, SOD, and GSH enzymes. Dietary supplementation of saffloweroil and vitamin C improved the intestinal histology. They increased the villous height and width, crypt depth, villous height/crypt depth ratio, mucosal thickness, goblet cell count, and intra-epithelium lymphocytic lick cell infiltrations. The serum levels of IgA and complement C3 were increased (P < 0.01) by Vit. C supplementation and prominent in the 400 vit. C + 10 safflowerOil group. CONCLUSION: A dietary combination of saffloweroil and vitamin C resulted in improved growth rate, amino acids AID%, intestinal histology, welfare, immune and antioxidant status of birds, and obtaining ω-3 and linoleic acid-enriched breast muscles. The best inclusion level was 400 vit. C + 10 safflowerOil.
Authors: Anaam E Omar; Hanan S Al-Khalaifah; Ali Osman; Ahmed Gouda; Shimaa I Shalaby; Elshimaa M Roushdy; Samar A Abdo; Sozan A Ali; Aziza M Hassan; Shimaa A Amer Journal: Antioxidants (Basel) Date: 2022-05-19
Authors: Miroslava Anna Šefcová; Francisco Santacruz; César Marcelo Larrea-Álvarez; Christian Vinueza-Burgos; David Ortega-Paredes; Gabriel Molina-Cuasapaz; Jessica Rodríguez; William Calero-Cáceres; Viera Revajová; Esteban Fernández-Moreira; Marco Larrea-Álvarez Journal: Animals (Basel) Date: 2021-12-19 Impact factor: 2.752
Authors: Shimaa A Amer; Hanan S Al-Khalaifah; Ahmed Gouda; Ali Osman; Nehal I A Goda; Haiam A Mohammed; Mahmoud I M Darwish; Aziza M Hassan; Sherif Kh A Mohamed Journal: Antioxidants (Basel) Date: 2022-03-13