Anthony J Deegan1, Jie Lu1, Rajendra Sharma1, Samuel P Mandell2, Ruikang K Wang1,3. 1. Department of Bioengineering, University of Washington, Seattle, WA, USA. 2. Division of Trauma, Critical Care, and Burn, University of Washington, Harborview Medical Center, Seattle, WA, USA. 3. Department of Ophthalmology, University of Washington, Seattle, WA, USA.
Abstract
BACKGROUND: Skin autografting is a common clinical procedure for reconstructive surgery. Despite its widespread use, very few studies have been conducted to non-invasively evaluate and monitor the vascular and structural features of skin grafts. This study, therefore, aims to demonstrate the potential of optical coherence tomography (OCT) alongside OCT-based angiography (OCTA) to non-invasively image and monitor human skin graft health and integration over time. METHODS: An in-house-built clinical prototype OCT system was used to acquire OCT/OCTA images from patients who underwent split-thickness skin graft surgery following severe burn damage to the skin. The OCT imaging was carried out at multiple locations over multiple time points with a field of view of ~9 mm × 9 mm and a penetration depth of ~1.5 mm. In addition to obtaining high-resolution qualitative images, we also quantitatively measured and compared specific structural and vascular parameters, such as identifiable layer thickness and corresponding vascular area density and diameter. RESULTS: Two patients (patient #1 and #2) were enrolled for this preliminary study. Vascular and structural features were successfully imaged and measured in the graft tissue and integration layer immediately beneath at different time points. Revascularization, healing, and integration were monitored with patient-specific details. Results of the quantitative image analysis from patient #1 indicated that integration layer thickness 16-day post-surgery was significantly less (~50%) than that of 7-day post-surgery. Additionally, with patient #2, significant growth (~20%) was seen with the vascular area density of both the graft and corresponding integration layer beneath between 6 and 14 days post-surgery. CONCLUSIONS: Our preliminary studies show that OCT/OCTA has clinical potential to image and measure numerous features of human skin graft health and integration in the days and weeks following split-thickness surgery. For the first time, we demonstrate the applicability of non-invasive imaging technology for novel clinical uses that could eventually aid in the betterment of surgical practices and clinical outcomes. 2021 Quantitative Imaging in Medicine and Surgery. All rights reserved.
BACKGROUND: Skin autografting is a common clinical procedure for reconstructive surgery. Despite its widespread use, very few studies have been conducted to non-invasively evaluate and monitor the vascular and structural features of skin grafts. This study, therefore, aims to demonstrate the potential of optical coherence tomography (OCT) alongside OCT-based angiography (OCTA) to non-invasively image and monitor human skin graft health and integration over time. METHODS: An in-house-built clinical prototype OCT system was used to acquire OCT/OCTA images from patients who underwent split-thickness skin graft surgery following severe burn damage to the skin. The OCT imaging was carried out at multiple locations over multiple time points with a field of view of ~9 mm × 9 mm and a penetration depth of ~1.5 mm. In addition to obtaining high-resolution qualitative images, we also quantitatively measured and compared specific structural and vascular parameters, such as identifiable layer thickness and corresponding vascular area density and diameter. RESULTS: Two patients (patient #1 and #2) were enrolled for this preliminary study. Vascular and structural features were successfully imaged and measured in the graft tissue and integration layer immediately beneath at different time points. Revascularization, healing, and integration were monitored with patient-specific details. Results of the quantitative image analysis from patient #1 indicated that integration layer thickness 16-day post-surgery was significantly less (~50%) than that of 7-day post-surgery. Additionally, with patient #2, significant growth (~20%) was seen with the vascular area density of both the graft and corresponding integration layer beneath between 6 and 14 days post-surgery. CONCLUSIONS: Our preliminary studies show that OCT/OCTA has clinical potential to image and measure numerous features of human skin graft health and integration in the days and weeks following split-thickness surgery. For the first time, we demonstrate the applicability of non-invasive imaging technology for novel clinical uses that could eventually aid in the betterment of surgical practices and clinical outcomes. 2021 Quantitative Imaging in Medicine and Surgery. All rights reserved.
Authors: O Timar-Banu; H Beauregard; J Tousignant; M Lassonde; P Harris; G Viau; L Vachon; E Levy; T Abribat Journal: Wound Repair Regen Date: 2001 Mar-Apr Impact factor: 3.617
Authors: Ruikang K Wang; Steven L Jacques; Zhenhe Ma; Sawan Hurst; Stephen R Hanson; Andras Gruber Journal: Opt Express Date: 2007-04-02 Impact factor: 3.894
Authors: Anthony J Deegan; Faezeh Talebi-Liasi; Shaozhen Song; Yuandong Li; Jingjiang Xu; Shaojie Men; Michi M Shinohara; Mary E Flowers; Stephanie J Lee; Ruikang K Wang Journal: Lasers Surg Med Date: 2018-01-22 Impact factor: 4.025
Authors: Walker D Short; Oluyinka O Olutoye; Benjamin W Padon; Umang M Parikh; Daniel Colchado; Hima Vangapandu; Shayan Shams; Taiyun Chi; Jangwook P Jung; Swathi Balaji Journal: Front Bioeng Biotechnol Date: 2022-09-23