Peizeng Jia1, Jianyin Zou2, Shankai Yin2, Feng Chen3, Hongliang Yi2, Qian Zhang3. 1. Department of Orthodontics, Peking University School and Hospital of Stomatology, Beijing, China. 2. Department of Otolaryngology Head and Neck Surgery and Center of Sleep Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China. 3. Central Laboratory, Peking University School and Hospital of Stomatology, Beijing, China.
Abstract
BACKGROUND: Oral microbiota plays an important role in oral and systemic diseases, while few reports referred to obstructive sleep apnea syndrome (OSAS). Thus, this study aimed to explore the different salivary microbiome in patients with OSAS and controls. MATERIALS AND METHODS: Saliva was collected from 15 OSAS patients and nine healthy controls, and bacterial genomic DNA was extracted for 16S rRNA amplicon sequencing based on the Illumina platform. RESULTS: The alpha and beta diversities were not significantly different between patients with OSAS and controls. The main phyla in the two groups were Firmicutes, Actinobacteria, Bacteroidetes, Proteobacteria, and Fusobacteria, which accounted for 95% of the abundance. The main genera were Streptococcus, Rothia, Actinomyces, Prevotella, and Neisseria. Based on the genus and operational taxonomic units, Peptostreptococcus, Alloprevotella, and Granulicatella were enriched in controls, while only Scardovia species were significantly more abundant in patients with OSAS. CONCLUSIONS: There was no significant difference in the relative abundance of bacteria between OSAS and controls. So, further studies will need to focus on the metagenome of bacteria in OSAS patients.
BACKGROUND: Oral microbiota plays an important role in oral and systemic diseases, while few reports referred to obstructive sleep apnea syndrome (OSAS). Thus, this study aimed to explore the different salivary microbiome in patients with OSAS and controls. MATERIALS AND METHODS: Saliva was collected from 15 OSAS patients and nine healthy controls, and bacterial genomic DNA was extracted for 16S rRNA amplicon sequencing based on the Illumina platform. RESULTS: The alpha and beta diversities were not significantly different between patients with OSAS and controls. The main phyla in the two groups were Firmicutes, Actinobacteria, Bacteroidetes, Proteobacteria, and Fusobacteria, which accounted for 95% of the abundance. The main genera were Streptococcus, Rothia, Actinomyces, Prevotella, and Neisseria. Based on the genus and operational taxonomic units, Peptostreptococcus, Alloprevotella, and Granulicatella were enriched in controls, while only Scardovia species were significantly more abundant in patients with OSAS. CONCLUSIONS: There was no significant difference in the relative abundance of bacteria between OSAS and controls. So, further studies will need to focus on the metagenome of bacteria in OSAS patients.
Authors: Isabel Moreno-Indias; Marta Torres; Lidia Sanchez-Alcoholado; Fernando Cardona; Isaac Almendros; David Gozal; Josep M Montserrat; Maria I Queipo-Ortuño; Ramon Farré Journal: Sleep Date: 2016-10-01 Impact factor: 5.849
Authors: Andre P Masella; Andrea K Bartram; Jakub M Truszkowski; Daniel G Brown; Josh D Neufeld Journal: BMC Bioinformatics Date: 2012-02-14 Impact factor: 3.169
Authors: Ying Dong; Pan Wang; Junling Lin; Chunming Han; Jie Jiao; Kun Zuo; Mulei Chen; Xinchun Yang; Jun Cai; He Jiang; Xiheng Guo; Jing Li Journal: J Clin Sleep Med Date: 2022-02-01 Impact factor: 4.062