Isabel Moreno-Indias1,2, Marta Torres3,4, Lidia Sanchez-Alcoholado1,2, Fernando Cardona1,2, Isaac Almendros4,5, David Gozal6, Josep M Montserrat3,4,7, Maria I Queipo-Ortuño1,2, Ramon Farré4,7,5. 1. Unidad de Gestión Clínica de Endocrinología y Nutrición, Hospital Virgen de la Victoria, Instituto de Investigación Biomédica de Málaga (IBIMA), Málaga, Spain. 2. Centro de Investigación Biomédica en Red de Fisiopatología de la Obesidad y la Nutrición, CIBER, Madrid, Spain. 3. Laboratori del Son, Hospital Clínic, Universitat de Barcelona, Barcelona, Spain. 4. Centro de Investigación Biomédica en Red de Enfermedades Respiratorias, CIBER, Madrid, Spain. 5. Unitat de Biofísica i Bioenginyeria, Facultat de Medicina, Universitat de Barcelona-IDIBAPS, Barcelona, Spain. 6. Department of Pediatrics, Biological Sciences Division, Pritzker School of Medicine, The University of Chicago, Chicago, IL. 7. Institut Investigacions Biomèdiques August Pi i Sunyer, Barcelona, Spain.
Abstract
STUDY OBJECTIVES: Intermittent hypoxia (IH) mimicking obstructive sleep apnea (OSA) significantly modifies gut microbiota in mice. However, whether these IH-induced gut microbiome changes are reversible after restoring normal oxygenation (the equivalent of effective OSA therapy) is unknown. The aim of this study was to investigate gut microbiota composition and circulating endotoxemia after a post-IH normoxic period in a mouse model of OSA. METHODS: Ten mice were subjected to IH (40 sec 21% O2-20 sec 5% O2) for 6 h/day for 6 w and 10 mice breathing normoxic air (NM) were used as controls. After exposures, both groups were subjected to 6 w in normoxia. Microbiome composition of fecal samples was determined by 16S ribosomal RNA (rRNA) pyrosequencing. Bioinformatic analysis was performed by Quantitative Insights into Microbial Ecology. Plasma lipopolysaccharide (LPS) levels were measured by endotoxin assay. RESULTS: After normoxic recovery, the Chao and Shannon indices of each group suggested similar bacterial richness and diversity. 16S rRNA pyrosequencing analysis showed that IH-exposed mice had a significant decrease in the abundance of Bacteroidetes and a significant increase of Firmicutes and Deferribacteres compared to the NM group. After normoxic recovery, circulating LPS concentrations were higher in the IH group (P < 0.009). Moreover, the IH group showed a negative and significant correlation between the abundance of Lactobacillus and Ruminococcus and significant positive correlations between the abundance of Mucispirillum and Desulfovibrio and plasma LPS levels, respectively. CONCLUSIONS: Even after prolonged normoxic recovery after IH exposures, gut microbiota and circulating endotoxemia remain negatively altered, suggesting that potential benefits of OSA treatment for reversing OSA-induced changes in gut microbiota may either require a longer period or alternative interventions.
STUDY OBJECTIVES: Intermittent hypoxia (IH) mimicking obstructive sleep apnea (OSA) significantly modifies gut microbiota in mice. However, whether these IH-induced gut microbiome changes are reversible after restoring normal oxygenation (the equivalent of effective OSA therapy) is unknown. The aim of this study was to investigate gut microbiota composition and circulating endotoxemia after a post-IH normoxic period in a mouse model of OSA. METHODS: Ten mice were subjected to IH (40 sec 21% O2-20 sec 5% O2) for 6 h/day for 6 w and 10 mice breathing normoxic air (NM) were used as controls. After exposures, both groups were subjected to 6 w in normoxia. Microbiome composition of fecal samples was determined by 16S ribosomal RNA (rRNA) pyrosequencing. Bioinformatic analysis was performed by Quantitative Insights into Microbial Ecology. Plasma lipopolysaccharide (LPS) levels were measured by endotoxin assay. RESULTS: After normoxic recovery, the Chao and Shannon indices of each group suggested similar bacterial richness and diversity. 16S rRNA pyrosequencing analysis showed that IH-exposed mice had a significant decrease in the abundance of Bacteroidetes and a significant increase of Firmicutes and Deferribacteres compared to the NM group. After normoxic recovery, circulating LPS concentrations were higher in the IH group (P < 0.009). Moreover, the IH group showed a negative and significant correlation between the abundance of Lactobacillus and Ruminococcus and significant positive correlations between the abundance of Mucispirillum and Desulfovibrio and plasma LPS levels, respectively. CONCLUSIONS: Even after prolonged normoxic recovery after IH exposures, gut microbiota and circulating endotoxemia remain negatively altered, suggesting that potential benefits of OSA treatment for reversing OSA-induced changes in gut microbiota may either require a longer period or alternative interventions.
Authors: Nanda Burger-van Paassen; Audrey Vincent; Patrycja J Puiman; Maria van der Sluis; Janneke Bouma; Günther Boehm; Johannes B van Goudoever; Isabelle van Seuningen; Ingrid B Renes Journal: Biochem J Date: 2009-05-13 Impact factor: 3.857
Authors: Anabel L Castro-Grattoni; Roger Alvarez-Buvé; Marta Torres; Ramon Farré; Josep M Montserrat; Mireia Dalmases; Isaac Almendros; Ferran Barbé; Manuel Sánchez-de-la-Torre Journal: Chest Date: 2016-01-13 Impact factor: 9.410
Authors: Isabel Moreno-Indias; Fernando Cardona; Francisco J Tinahones; María Isabel Queipo-Ortuño Journal: Front Microbiol Date: 2014-04-29 Impact factor: 5.640