Transition Metal-Free N-Arylation of Amino Acid Esters with Diaryliodonium Salts.

A transition metal-free approach for the N-arylation of amino acid derivatives has been developed. Key to this method is the use of unsymmetric diaryliodonium salts with anisyl ligands, which proved important to obtain high chemoselectivity and yields. The scope includes the transfer of both electron deficient, electron rich and sterically hindered aryl groups with a variety of different functional groups. Furthermore, a cyclic diaryliodonium salt was successfully employed in the arylation. The N-arylated products were obtained with retained enantiomeric excess.

Introduction

Amino acids are important building blocks in organic synthesis as they are a substantial part of the chiral pool. Functionalization of such species is attractive in drug discovery as a mean of obtaining novel compounds that can act as an affinity probe for enzyme studies or exhibit bioactivity. N‐Arylated amino acids are found as a core structure in biologically active compounds such as the protein kinase C (PKC) activator indolactam‐V and its analogue benzolactam‐V8, fibrinogen receptor antagonist n>an class="Chemical">SB 214857, and NMDA receptor antagonist L689560.

Existing methods to reach N‐aryl amino acids are mostly based on transition metal catalysis. n>an class="Chemical">Cu‐catalyzed Ullman cross couplings have a good scope, although high Cu loading and extended reaction times at high temperatures or excess reagents are often needed.[ , , ] Jain and co‐workers recently reported an N‐arylation under milder conditions with the use of a diketone ligand in DMF (Scheme 1 a). After adjustment of the reaction conditions, also heterocycles could be transferred. Alternatively, Cu‐catalyzed couplings with excess arylboronic acid can be performed at room temperature with a limited scope.

Scheme 1

Recent N‐arylations of amino acid derivatives and amines.

Recent N‐arylations of amino acid derivatives and pan class="Chemical">amines.

pan class="Chemical">Pd‐catalyzed Buchwald‐Hartwig arylations have been less explored, and early methods suffered from limited scope or partial racemization. Recent repn>orts partly cirn>an class="Chemical">cumvent that problem through the use of advanced catalytic systems.

The development transition metal‐free coupn>ling reactions has received increasing attention to overcome drawbacks with transition metals, such as toxicity, cost, need for substrate‐dependent designer ligands, reaction sensitivity and the risk of product contamination in the pharmaceutical industry. Transition metal‐free syntheses of N‐aryl amino acids and their derivatives are, however, much less explored. Reported syntheses of specific targets include reactions with anilines and suitable electrophilic species through SN2 displacement or SNAr. Reactions with enantiomerically enriched trichloromethyl alcohols provided N‐aryl amino amides through a Jocic‐type reaction. Alternatively, amino acid derivatives can be N‐arylated with the biobased reagent methyl‐3‐dehydroshikimate as aryl precursor. Triphenylsulfonium triflate can be employed in the N‐arylation of racemic amino acid derivatives in a reaction proceeding through arynes. A common feature with the methods above is that their scope is very limited.

Poelarends and co‐workers recently published an elegant biocatalytic approach to N‐arylated aspn>artic acids through enzymatic reactions of n>an class="Chemical">anilines with fumarate. Moderate to good yields with excellent ee were obtained, although sterically hindered or strongly electron deficient anilines were not tolerated.

Hypervalent iodine compounds have become efficient reagents for a wide variety of transition n>an class="Chemical">metal‐free reactions. Diaryliodonium salts have several attractive features including easy availability, high stability, and low toxicity. They are highly reactive electrophilic arylation reagents, and have been applied successfully in a variety of transition metal‐free C‐, O‐, N‐ and S‐arylations.

Iodonium salts have been utilized to functionalize amino acid derivatives through fluoroalkylation, and a small set of n>an class="Chemical">diaryliodonium bromides were employed in a Cu‐catalyzed N‐arylation of amino acid esters, which required excess substrate and a stochiometric amount of AgNO3 (Scheme 1 b). We have recently reported a metal‐free N‐arylation of aliphatic amines with diaryliodonium salts under mild conditions (Scheme 1 c). The methodology has a broad amine scope, but proved inefficient for arylation of amino acids. To facilitate the access to enantiomerically enriched N‐arylated amino acid derivatives, we set out to develop a transition metal‐free N‐arylation of amino acids derivatives, and herein present our results.

Results and Discussion

Phenylalanine methyl ester (1 a) was chosen as the model substrate, and was obtained by depn>rotonation of the correspn>onding hydrochloride n>an class="Chemical">salt 1 a‐HCl. The free amine 1 a proved to be unstable upon storage,[ , ] and was therefore prepared within 5 days of use.

An extensive optimization was performed, with initial screening of the arylation conditions using 4‐nitrophenyl(phenyl)iodonium n>an class="Chemical">triflate (2 aa‐OTf). The conditions used in our arylation of aliphatic amines gave poor conversion into product 3 a with substantial amounts of recovered 1 a (Table 1, entry 1). The conversion was improved by increasing the temperature (entries 2,3), and the combination with excess iodonium salt resulted in 59 % yield of 3 a (entry 4). Arylations with iodonium salt 2 aa‐OTf generally give complete chemoselectivity, with transfer of only the nitroaryl group, but we observed small amounts of phenylated side‐product 3‐Ar and a deterioration of the overall mass balance when the reaction was performed at 150 °C.

Table 1

Optimization of the 4‐nitrophenylation of 1 a.[a]

Entry	Salt 2 a‐X			T	t	Yield [%][b] of
		Ar2	(equiv)	[°C]	[h]	3 a	3‐Ar2
1	2 aa‐OTf	Ph	1.0	110	22	16 (15)	0
2	2 aa‐OTf	Ph	1.0	130	22	(28)	0
3	2 aa‐OTf	Ph	1.0	150	4	47 (45)	(6)
4	2 aa‐OTf	Ph	2.0	150	4	59	12
5	2 aa‐BF4	Ph	2.0	150	4	34	14
6[c]	2 aa‐OTs	Ph	2.0	150	4	12 (14)	(12)
7[d]	2 aa‐Br	Ph	2.0	150	4	0	0
8	2 ab‐OTf	Mesityl	2.0	150	4	49	10
9	2 ac‐OTf	Anisyl	2.0	150	4	85 (79)	0
10	2 ac‐OTf	Anisyl	1.5	150	4	66	0
11	2 ac‐OTf	Anisyl	1.0[e]	150	4	73	0
12	2 ac‐OTf	Anisyl	2.0	130	4	78 (77)	0
13	2 ac‐OTf	Anisyl	2.0	110	24	60	0

[a] Reaction conditions: 1 a (0.2 mmol, 1 equiv), 2 a‐X (1–2 equiv) and base (1 equiv) were mixed under argon. Degassed, anhydrous toluene (1 mL) was added, and the reaction was heated in an oil bath with stirring. [b] 1H NMR yield with trimethoxybenzene (TMB) as internal standard, isolated yields given in parentheses. [c]>95 % 4‐OTs formed. [d]>95 % 4‐Br formed. [e] 2.0 equiv of 1 a and base were used.

pan class="Chemical">Salt 2 a‐X

3‐Ar

2 aa‐BF

[a] Reaction conditions: 1 a (0.2 mmol, 1 equiv), 2 a‐X (1–2 equiv) and base (1 equiv) were mixed under argon. Degassed, anhydrous n>an class="Chemical">toluene (1 mL) was added, and the reaction was heated in an oil bath with stirring. [b] 1H NMR yield with trimethoxybenzene (TMB) as internal standard, isolated yields given in parentheses. [c]>95 % 4‐OTs formed. [d]>95 % 4‐Br formed. [e] 2.0 equiv of 1 a and base were used.

The reactivity of 4‐nitrophenyl(phenyl)iodonium salts with other anions (2 aa‐X) was next exn>an class="Chemical">amined, and salts with tetrafluoroborate, tosylate or bromide anions proved to give inferior results (entries 5–7). In fact, iodonium salts 2 aa‐OTs and 2 aa‐Br suffered from a competing pathway where the anion acted as nucleophile to deliver the corresponding 4‐nitrophenyl tosylate (4‐OTs) and bromide (4‐Br), respectively. Such side‐products have previously been reported with diaryliodonium bromides, whereas reactions with diaryliodonium tosylates are often efficient also at elevated temperatures.[ , , ]

Unsymmetric diaryliodonium salts are known to react with high chemoselectivity when they have sufficiently different electronic properties. The non‐transferable aryl groupn> is called a “dummy groupn>”, and the phenyl groupn> is generally a sufficient dummy groupn> in transfer of strongly electron‐deficient aryl groupn>s. Since the phenylated side‐product was observed in this reaction, we investigated n>an class="Chemical">iodonium salts with other dummy groups to improve the chemoselectivity and simplify the isolation of product 3 a. Reactions with mesityl salt 2 ab‐OTf resulted in similar yield and chemoselectivity (entry 8). The anisyl moiety is often a good dummy group and salt 2 ac‐OTf indeed reacted with complete chemoselectivity. More surprisingly, the conversion was also improved and 3 a was isolated in 79 % yield as the only product (entry 9).

Further investigations with salt 2 ac‐OTf showed that changes in reaction stoichiometry had a negative impact (entries 10, 11). Comparable results were obtained at 130 °C (entry 12), and the reaction could even be performed at 110 °C with this n>an class="Chemical">salt, illustrating the reactivity difference to salt 2 aa‐OTf (entry 13 vs. 1). Importantly, the enantiomeric excess of product 3 a was >98 %, demonstrating that the reaction conditions were mild enough to not cause racemization despite the high reaction temperature.

We next examined whether the depn>rotonation of 1 a‐n>an class="Chemical">HCl could be combined with the arylation to circumvent the handling of unstable amine 1 a. 1 a‐HCl was thus reacted with salt 2 ac‐OTf (1–2 equiv) in the presence of 2 equiv sodium carbonate (Scheme 2). However, only minor amounts of 3 a were detected and the major product was instead 4‐Cl, which formed when the released chloride anion acted as a competing nucleophile. The mass balance in the first reaction shows that 1 a is rather stable at elevated temperatures once 2 ac‐OTf is consumed, but partly decomposes in the presence of excess 2 ac‐OTf, likely due to the high oxidation potential of the diaryliodonium salt.

Scheme 2

Attempted arylation of amino acid ester salt 1 a‐HCl (1H NMR yields with TMB as internal standard).

Attempted arylation of amino acid ester n>an class="Chemical">salt 1 a‐HCl (1H NMR yields with TMB as internal standard).

The arylation scope of amino ester 1 a with various n>an class="Chemical">aryl(anisyl)iodonium triflates 2‐OTf was subsequently examined. Aryl groups with electron‐withdrawing groups (EWG) were efficiently transferred using the optimized conditions (Scheme 3 A), as exemplified by the synthesis of p‐CN substituted products 3 b and 3 c. Aryl groups with CF3 substituents were also well tolerated (3 d–3 f), where even the sterically encumbered product 3 f was formed in high yield. Also halogen‐containing aryl moieties could be transferred to provide 3 f–3 h. Iodonium salts with strong EWG reacted with similar efficiency at 130 and 150 °C while the other salts showed reduced activity at 130 °C.

Scheme 3

Arylation scope with amino acid esters 1, ee values 95–98 % unless stated. [a] Reaction at 130 and 150 °C gave similar results. [b] Symmetric salt 2 (Ar1=Ar2) used.

Arylation scope with pan class="Chemical">amino acid esters 1, ee values 95–98 % unless stated. [a] Reaction at 130 and 150 °C gave similar results. [b] Symmetric n>an class="Chemical">salt 2 (Ar1=Ar2) used.

The transfer of aryls with electron‐donating groups (EDG) is generally more challenging in reactions with n>an class="Chemical">diaryliodonium salts.[ , ] We were hence pleased to see that such arylations were feasible by prolonging the reaction time to 24 h. In this fashion, the phenylated product 3 i was isolated in 67 % yield, and a tert‐butyl‐substituted aryl moiety could also be transferred to provide 3 j. The method demonstrated good compatibility with the sterically demanding mesityl group (3 k) and a highly functionalized aryl moiety could be transferred (3 l). A heteroaryliodonium salt was employed to give pyridyl product 3 m in modest yield. Additionally, the 6‐membred cyclic diaryliodonium salt 2 n could be used to generate the iodo‐substituted product 3 n in 59 % yield. Reactions with cyclic diaryliodonium salts generally require transition metal catalysis due to decreased reactivity, and this result is conceptually important as reactions with such salts have higher atom efficiency and deliver products with a convenient iodine handle for further transformations.

The substrate scope of primary amino esters 1 was next exn>an class="Chemical">amined. Arylation of benzyl ester 1 b was first investigated and provided the arylated products 3 o and 3 p in equally high yields as the corresponding methyl ester 1 a (cf. products 3 a, 3 b). Having demonstrated the compatibility of the benzyl protecting group, it was used to protect alanine (1 c) and valine (1 d), as their corresponding methyl esters (1 e, 1 f) proved to be volatile and easily evaporated under vacuum or high temperatures. Arylation of the alanine and valine benzyl esters was successful providing 3 q and 3 r in moderate to good yields. Substrates 1 c and 1 d could also be arylated with less activated diaryliodonium salts, providing the phenylated and tert‐butyl‐substituted products 3 s–3 v. The reactions with valine ester 1 d gave consistently higher yields than the corresponding reactions with 1 c, a trend that has also been reported in previous N‐arylations.[ , , ]

We next explored the compatibility with more challenging substrates, such as unprotected, heteroatom‐substituted amino acid esters. Heteroatom substituents are usually well tolerated in n>an class="Chemical">metal‐free arylations with diaryliodonium salts, but such substrates proved difficult to arylate under the current conditions. For example, reactions with tryptophan methyl ester (1 g) gave only 15 % product with 2 ac‐OTf, which could be due to competing coordination of the indole nitrogen to the iodine(III) reagent. To the contrary, arylation of tyrosine methyl ester (1 h) delivered the diarylated product 3 w′ in high yield and retained ee, without detection of the corresponding monoarylated product 3 w (Scheme 4).

Scheme 4

Diarylation of tyrosine methyl ester.

Diarylation of pan class="Chemical">tyrosine methyl ester.

To investigate the performance of the unsymmetric anisyl salts, a few products in the scope were also synthesized using the correspn>onding n>an class="Chemical">aryl(phenyl) salts or symmetric diaryliodonium salts. Reagents with the anisyl dummy generally performed best, as chemoselectivity problems were avoided and better arylation yields were obtained.

SFC and HPLC analyses were performed to determine the ee of the products, since previously repn>orted methods for N‐arylation of n>an class="Chemical">amino esters showed partial racemization[ , , , ] or had insufficient data to judge the enantiomeric purity.[ , , , ] As expected, the current methodology generally left the existing stereocenter intact and the majority of the products were isolated with 95 to >98 % ee. Even when prolonged reaction time was applied in reactions with EDG salts, very little racemization occurred.

Secondary amino esters were evaluated next, and n>an class="Chemical">proline methyl ester (5 a) proved to be a suitable substrate (Scheme 5). Arylation with electron‐withdrawing aryl moieties provided products 6 a‐‐6 c in high yields. Upon prolonged reaction time, also phenylation (6 d) and mesitylation (6 e) was feasible, these transformations proved better with the symmetric iodonium salts than with the anisyl salts. N‐arylation of the N‐methylated α‐phenylalanine ester 5 b resulted in 58 % yield of 6 f with high enantiomeric excess, and β‐phenylalanine ester (5 c) gave the nitrophenylated product 6 g in good yield. The SFC analysis of products 6 showed a small decrease of the enantiomeric purity (90–94 % ee).

Scheme 5

Arylation of amino acid esters 5. [a] Reaction at 130 °C. [b] Symmetric salt 2 (Ar1=Ar2) used, 24 h reaction time.

Arylation of pan class="Chemical">amino acid esters 5. [a] Reaction at 130 °C. [b] Symmetric n>an class="Chemical">salt 2 (Ar1=Ar2) used, 24 h reaction time.

N‐Protected derivatives of 1 a,b remained largely untouched under the reaction conditions, which is useful in arylation of more complex substates. To this end, n>an class="Chemical">acetyl‐ and tosyl‐protected derivatives of 1 a gave no arylation, whereas a Boc‐protected derivative of 1 b gave 9 % of 3 o with 82 % recovered substrate, meaning that the Boc‐group had been partially fragmented under the reaction conditions.

A series of control experiments were performed to indicate a possible mechanism of the arylation. Running the reaction without argon atmospn>here gave a significantly lower yield. Addition of n>an class="Chemical">DPE as radical trap did not affect the outcome of the reaction, indicating that a radical pathway is unlikely. Furthermore, reactions in the presence of furan (5 equiv) as aryne trap did not result in formation of Diels–Alder adducts. Based on that experiment and the formation of only one regioisomeric product in reactions with substituted diaryliodonium salts, an aryne pathway can be excluded. Additionally, an attempted arylation of 1 a with iodobenzene or 1‐iodo‐4‐nitrobenzene at 150 °C resulted in quantitative recovery of the starting materials, and an SNAr pathway with the iodoarene formed from 2 aa‐OTf can therefore be ruled out.

Finally, the product stability was investigated by subjecting 3 a to the reaction conditions (Scheme 6). No diarylated product was formed and 3 a could be recovered in 68 % yield. The ee of 3 a remained intact, demonstrating that the arylated product is stable to racemization under the reaction conditions. However, partial decomposition of 3 a had ocpan class="Chemical">curred, which explains the mass balance problems observed in some reactions. A stability test of 2 ac‐OTf in the absence of any n>an class="Chemical">amino acid ester also showed partial decomposition at 150 °C.

Scheme 6

Stability test of 3 a.

Based on the experiments described above, we suggest that the reaction follows a traditional ligand coupling pathway, where the pan class="Chemical">amine does a ligand exchange with the n>an class="Chemical">triflate to give intermediate I, followed by deprotonation to II and ligand coupling to yield product 3 (Scheme 7).

Scheme 7

Suggested mechanism.

Conclusions

A transition metal‐free N‐arylation of amino acid derivatives with n>an class="Chemical">diaryliodonium salts has been presented. The moderate reactivity of the substrates was overcome by increasing the reaction temperature, which initially resulted in a chemoselectivity problem. The method has a broad arylation scope and is compatible with transfer of both electron deficient and electron rich aryl groups in good to high yields. ortho‐Substituents and various functional groups are well tolerated, including a cyclic diaryliodonium salt. The amino acid ester scope includes primary and secondary α‐amino esters, as well as β‐amino esters, and the products were generally obtained with excellent enantiomeric excess. The use of aryl(anisyl)iodonium triflates proved to be key to increase both the reactivity and chemoselectivity of the process. This interesting observation will be further studied in reactions with related nucleophiles.

Experimental Section

Arylation of amino acid esters 1 and 5: n>an class="Chemical">Amino acid ester 1 (0.2 mmol), salt 2 (0.4 mmol, 2.0 equiv) and Na2CO3 (0.2 mmol, 1.0 equiv) were added to an oven‐dried, pressure‐stable microwave vial and dried under vacuum for 15 min. The vial was flushed with argon 3–4 times followed by the addition of anhydrous toluene (1 mL, degassed by bubbling with argon for 20 min). The vial was added to a preheated oil bath at 150 °C, and stirred for 4–24 h. After completion, the reaction was cooled to rt and Celite was added. The volatiles were removed under reduced pressure, and the mixture was purified by column chromatography (SiO2 with pentane/EtOAc as eluent system), to provide product 3 or 6. The enantiomeric purity was analyzed by Chiral SFC, Diacel OJ‐H, 25 °C, 0.3 cm Ø, 15 cm column, 10 % MeOH in CO2, flow rate: 0.8 mL min−1.

Conflict of interest

The authors declare no conflict of interest.

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