| Literature DB >> 33458604 |
Muhammad Zaka Asif1, Victoria L Van der Gaag1, Jane Guo1, Kelsey A Nocilla1, Cole J Muzio1, Arthur S Edison1,2,3.
Abstract
Caenorhabditis elegans is an ideal model organism for studying the xenobiotic detoxification pathways of various natural and synthetic toxins. We developed a new workflow to study the effects of 1-hydroxyphenazine (1-HP), a toxin produced by the bacterium Pseudomonas aeruginosa, on the survival of C. elegans. Prior research has demonstrated that C. elegans can detoxify 1-HP through the general mechanism of O-glycosylation. As part of the Vertically Integrated Projects (VIP) undergraduate research team, we have developed a workflow for a plate-based toxicity assay to determine the median lethal dose (LD50) of 1-HP. This was achieved through a toxin exposure method in which the worms were exposed to varying concentrations of 1-HP. The death rates were measured using a fluorescent bead assay. This workflow can be used to test C. elegans responses to different toxins and also the response of different mutant strains to a toxin of interest. Copyright:Entities:
Year: 2021 PMID： 33458604 PMCID： PMC7807258 DOI： 10.17912/micropub.biology.000352
Source DB: PubMed Journal: MicroPubl Biol ISSN： 2578-9430