Krzysztof M Tokarz1, Wojciech Wesołowski2, Barbara Tokarz1, Wojciech Makowski1, Anna Wysocka1,3, Roman J Jędrzejczyk4, Karolina Chrabaszcz5, Kamilla Malek5, Anna Kostecka-Gugała2. 1. Department of Botany, Physiology and Plant Protection, Faculty of Biotechnology and Horticulture, University of Agriculture in Krakow, 29 Listopada 54, 31-425 Krakow, Poland. 2. Department of Plant Biology and Biotechnology, Faculty of Biotechnology and Horticulture, University of Agriculture in Krakow, 29 Listopada 54, 31-425 Krakow, Poland. 3. Centre Algatech, Institute of Microbiology, Czech Academy of Sciences, 379 81 Třeboň, Czech Republic. 4. Plant-Microorganism Interactions Group, Malopolska Centre of Biotechnology, Jagiellonian University, Gronostajowa 7A, 30-387 Krakow, Poland. 5. Raman Imaging Group, Faculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387 Krakow, Poland.
Abstract
Grass pea (Lathyrus sativus) is a leguminous plant of outstanding tolerance to abiotic stress. The aim of the presented study was to describe the mechanism of grass pea (Lathyrus sativus L.) photosynthetic apparatus acclimatisation strategies to salinity stress. The seedlings were cultivated in a hydroponic system in media containing various concentrations of NaCl (0, 50, and 100 mM), imitating none, moderate, and severe salinity, respectively, for three weeks. In order to characterise the function and structure of the photosynthetic apparatus, Chl a fluorescence, gas exchange measurements, proteome analysis, and Fourier-transform infrared spectroscopy (FT-IR) analysis were done inter alia. Significant differences in the response of the leaf and stem photosynthetic apparatus to severe salt stress were observed. Leaves became the place of harmful ion (Na+) accumulation, and the efficiency of their carboxylation decreased sharply. In turn, in stems, the reconstruction of the photosynthetic apparatus (antenna and photosystem complexes) activated alternative electron transport pathways, leading to effective ATP synthesis, which is required for the efficient translocation of Na+ to leaves. These changes enabled efficient stem carboxylation and made them the main source of assimilates. The observed changes indicate the high plasticity of grass pea photosynthetic apparatus, providing an effective mechanism of tolerance to salinity stress.
Grass pea (pecies">Lathyrus sativus) is a leguminous planpan>t of outstanpan>ding toleranpan>ce to pan> class="Disease">abiotic stress. The aim of the presented study was to describe the mechanism of grass pea (Lathyrus sativus L.) photosynthetic apparatus acclimatisation strategies to salinity stress. The seedlings were cultivated in a hydroponic system in media containing various concentrations of NaCl (0, 50, and 100 mM), imitating none, moderate, and severe salinity, respectively, for three weeks. In order to characterise the function and structure of the photosynthetic apparatus, Chl a fluorescence, gas exchange measurements, proteome analysis, and Fourier-transform infrared spectroscopy (FT-IR) analysis were done inter alia. Significant differences in the response of the leaf and stem photosynthetic apparatus to severe saltstress were observed. Leaves became the place of harmful ion (Na+) accumulation, and the efficiency of their carboxylation decreased sharply. In turn, in stems, the reconstruction of the photosynthetic apparatus (antenna and photosystem complexes) activated alternative electron transport pathways, leading to effective ATP synthesis, which is required for the efficient translocation of Na+ to leaves. These changes enabled efficient stem carboxylation and made them the main source of assimilates. The observed changes indicate the high plasticity of grass pea photosynthetic apparatus, providing an effective mechanism of tolerance to salinity stress.
Entities:
Keywords:
Lathyrus sativus; ROS; cyclic electron transport; linear electron transport; photosynthetic apparatus; photosystem I; photosystem II; salt stress
Authors: W J Nawrocki; B Bailleul; D Picot; P Cardol; F Rappaport; F-A Wollman; P Joliot Journal: Biochim Biophys Acta Bioenerg Date: 2019-03-07 Impact factor: 3.991
Authors: Karolina Miernicka; Barbara Tokarz; Wojciech Makowski; Stanisław Mazur; Rafał Banasiuk; Krzysztof M Tokarz Journal: Cells Date: 2022-09-27 Impact factor: 7.666