Cell signaling under salt, water and cold stresses.

Forward genetics and biochemical approaches to studying plant responses to salt, water and cold stresses began to bear fruit recently. Analysis of salt overly sensitive (sos) Arabidopsis mutants revealed a novel calcium-regulated protein kinase pathway for response to the ionic aspect of salt stress. In-gel kinase assays identified several SOS-independent protein kinases that are either activated specifically by osmotic stress or by multiple abiotic and biotic stresses. Molecular analysis revealed a transcriptional cascade in cold-regulated gene expression.